A5085604733- Advanced biosensing and bioanalysis techniques
- Analytical Chemistry and Sensors
- Electrochemical sensors and biosensors
- Carbon Nanotubes in Composites
- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- Graphene and Nanomaterials Applications
- Microfluidic and Bio-sensing Technologies
- Acoustic Wave Resonator Technologies
- RNA and protein synthesis mechanisms
- Mechanical and Optical Resonators
- Microfluidic and Capillary Electrophoresis Applications
- Cell Image Analysis Techniques
- Advanced Nanomaterials in Catalysis
- Lipid Membrane Structure and Behavior
- Supramolecular Self-Assembly in Materials
- Glycosylation and Glycoproteins Research
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- CAR-T cell therapy research
- Conducting polymers and applications
- Advanced Sensor and Energy Harvesting Materials
- Machine Learning in Bioinformatics
- Carbon and Quantum Dots Applications
- Biosensors and Analytical Detection
Iowa State University
2018-2025
Bissell (United States)
2018-2025
Ames National Laboratory
2024
Massachusetts Institute of Technology
2010-2015
IIT@MIT
2012
Hanyang University
2011
Polytechnic University of Turin
2011
Sandia National Laboratories California
2007
Temporal and spatial changes in neurotransmitter concentrations are central to information processing neural networks. Therefore, biosensors for neurotransmitters essential tools neuroscience. In this work, we applied a new technique, corona phase molecular recognition (CoPhMoRe), identify adsorbed polymer phases on fluorescent single-walled carbon nanotubes (SWCNTs) that allow the selective detection of specific neurotransmitters, including dopamine. We functionalized suspended SWCNTs with...
We report the selective detection of single nitric oxide (NO) molecules using a specific DNA sequence d(AT)(15) oligonucleotides, adsorbed to an array near-infrared fluorescent semiconducting single-walled carbon nanotubes (AT(15)-SWNT). While SWNT suspended with eight other variant sequences show fluorescence quenching or enhancement from analytes such as dopamine, NADH, L-ascorbic acid, and riboflavin, imparts distinct selectivity toward NO. In contrast, electrostatically neutral polyvinyl...
A class of peptides from the bombolitin family, not previously identified for nitroaromatic recognition, allows near-infrared fluorescent single-walled carbon nanotubes to transduce specific changes in their conformation. In response binding species, such peptide–nanotube complexes form a virtual “chaperone sensor,” which reports modulation peptide secondary structure via nanotubes, photoluminescence. split-channel microscope constructed image quantized spectral wavelength shifts real time,...
Cell-free expression (CFE) has shown recent utility in prototyping enzymes for discovery efforts. In this work, CFE is demonstrated as an effective tool to screen putative polyester polyurethane degrading enzyme sequences sourced from metagenomic analysis of biofilms prospected on aircraft and vehicles. An automated fluid handler with a controlled temperature block used assemble the numerous 30 µL reactions provide more consistent results over human assembly. sum, 13 hydrolase biofilm...
Excessive sample volumes continue to be a major limitation in the analysis of protein–protein interactions, motivating search for label-free detection methods greater sensitivity. Herein, we report first chemical approach selective protein recognition using fluorescent single-walled carbon nanotubes (SWNTs) enabling microarrays capable single detection. Hexahistidine-tagged capture proteins directly expressed by cell-free synthesis on SWNT/chitosan microarray are bound Ni2+ chelated...
Patients with chest pain account for 10% of US emergency room visits according to data from the Center Disease Control and Prevention (2013). For triage these patients, cardiac biomarkers troponin I T are endorsed as standard indicators acute myocardial infarction (AMI, or heart attack). Thus, there is significant interest in developing a rapid, point‐of‐care (POC) device detection. In this work, quantitative, label‐free assay, which specific (cTnT) detection, using fluorescent single‐walled...
Protein A is often used for the purification and detection of antibodies such as immunoglobulin G (IgG) because its quadrivalent domains that bind to Fc region these macromolecules. However, kinetics thermodynamics binding many sensor surfaces have eluded mechanistic description due complexities associated with multivalent interactions. In this work, we use a near-infrared (nIR) fluorescent single-walled carbon nanotube array obtain IgG protein A, immobilized using chelated Cu(2+)/His-tag...
Cell-free protein synthesis (CFPS) is an established biotechnology tool that has shown great utility in many applications such as prototyping proteins, building genetic circuits, designing biosensors, and expressing cytotoxic proteins. Although CFPS been widely deployed, the many, varied methods presented literature can be challenging for new users to adopt. From our experience others who newly enter field, one of most frustrating aspects applying a laboratory large levels variability are...
Enzymatic biodegradation is a promising method to reclaim plastic materials. However, date, high-throughput for screening potential enzyme candidates still lacking. Here, we propose single-walled carbon nanotube (SWCNT) fluorescence sensor the enzymatic degradation of polyester polyurethane nanoparticles. Through wrapping SWCNT with cationic chitosan, an electrostatic bond formed between and Impranil, widely applied model substrate polyurethane. As Impranil being degraded by enzymes,...
Reactive oxygen species, specifically hydrogen peroxide (H(2)O(2)), activate signal transduction pathways during angiogenesis and therefore play an important role in physiological development as well various pathophysiologies. Herein, we utilize a near-infrared fluorescent single-walled carbon nanotube (SWNT) sensor array to measure the single-molecule efflux of H(2)O(2) from human umbilical vein endothelial cells (HUVEC) response angiogenic stimulation. Two agents were investigated:...
Advancements in optical nanosensor development have enabled the design of sensors using synthetic molecular recognition elements through a recently developed method called Corona Phase Molecular Recognition (CoPhMoRe). The resulting from these principles are highly selective for specific analytes, and demonstrate remarkable stability use under variety conditions. An essential element hinges on ability to understand interface between nanoparticles associated corona phase surrounding...
There is significant interest in developing new detection platforms for characterizing glycosylated proteins, despite the lack of easily synthesized model glycans or high affinity receptors this analytical problem. In work, we demonstrate a sensor array employing recombinant lectins as glycan recognition sites tethered via Histidine tags to Ni(2+) complexes that act fluorescent quenchers semiconducting single-walled carbon nanotubes (SWNTs) embedded chitosan hydrogel spot measure binding...
Phenyl boronic acids (PBA) are important binding ligands to pendant diols useful for saccharide recognition. The aromatic ring can also function anchor an otherwise hydrophilic polymer backbone the surface of hydrophobic graphene or carbon nanotube. In this work, we demonstrate both functions using a homologous series seven phenyl conjugated polyethylene glycol, eight-membered, branched (PPEG8) that allows aqueous dispersion single-walled nanotubes (SWNT) and quenching near-infrared...
It is widely recognized that an array of addressable sensors can be multiplexed for the label-free detection a library analytes. However, such arrays have useful properties emerge from ensemble, even when monofunctionalized. As examples, we show nanosensors estimate mean and variance observed dissociation constant (KD), using three different examples binding IgG with Protein A as recognition site, including polyclonal human (KD μ = 19 μM, σ2 1000 mM2), murine 4.3 nM, 3 μM2), CHO cells 2.5...
Three-dimensional tracking of single-walled carbon nanotubes (SWNT) with an orbital microscope is demonstrated. We determine the viscosity regime (above 250 cP) at which rotational diffusion coefficient can be used for length estimation. also demonstrate SWNT within live HeLa cells and use these findings to spatially map corral volumes (0.27-1.32 μm(3)), active transport velocity (455 nm/s), calculate local viscosities (54-179 cell. With respect future SWNTs as sensors in living cells, we...
Abstract In this study, we present a minimal template design and accompanying methods to produce assayable quantities of custom sequence proteins within 24 hr from receipt inexpensive gene fragments DNA synthesis vendor. This is done without the conventional steps plasmid cloning or cell‐based amplification expression. Instead linear PCR amplified, circularized, isothermally amplified using rolling circle polymerase. The resulting can be used directly with cost‐optimized,...