A5103146681- Advanced Fluorescence Microscopy Techniques
- Photoacoustic and Ultrasonic Imaging
- Optical Coherence Tomography Applications
- 3D Printing in Biomedical Research
- Orbital Angular Momentum in Optics
- Microfluidic and Bio-sensing Technologies
- Cellular Mechanics and Interactions
- Neuroscience and Neural Engineering
- Image Processing Techniques and Applications
- Optical measurement and interference techniques
- Tissue Engineering and Regenerative Medicine
- Plasmonic and Surface Plasmon Research
- Cardiomyopathy and Myosin Studies
- Cell Image Analysis Techniques
- Photonic and Optical Devices
- Advanced Electron Microscopy Techniques and Applications
- Advanced Measurement and Metrology Techniques
- Image and Signal Denoising Methods
- Osteoarthritis Treatment and Mechanisms
- Digital Holography and Microscopy
- Advanced Biosensing Techniques and Applications
- Force Microscopy Techniques and Applications
- Advanced Vision and Imaging
- Advanced Optical Sensing Technologies
- Cardiac Valve Diseases and Treatments
Clemson University
2015-2024
Shenzhen University
2022
Chinese University of Hong Kong
2022
Bellingham Technical College
2020
Medical University of South Carolina
2016
Bruce Technologies (United States)
2006
University of Minnesota
2002-2004
Twin Cities Orthopedics
2002
University of Miami
1996-2001
Abstract Surface plasmon resonance (SPR) biosensor is a powerful tool for studying the kinetics of biomolecular interactions because they offer unique real-time and label-free measurement capabilities with high detection sensitivity. In past two decades, SPR technology has been successfully commercialized its performance continuously improved lots engineering efforts. this review, we describe recent advances in technologies. The developments technologies focusing on speed, sensitivity,...
Optothermal nanotweezers have emerged as an innovative optical manipulation technique in the past decade, which revolutionized classical by efficiently capturing a broader range of nanoparticles. However, optothermal temperature field was merely employed for in-situ nanoparticles, its potential identifying bio-nanoparticles remains largely untapped. Hence, based on synergistic effect and CRIPSR-based bio-detection, we developed CRISPR-powered (CRONT). Specifically, harnessing...
The measurement accuracy of fringe projection profilometry (FPP) largely depends on the calibration procedure. A more reliable approach based stereo vision model FPP scheme in conjunction with bundle adjustment strategy is presented. It can adjust coordinates benchmarks and thereby estimate parameters accurately even an imperfect target. experiment results shows that proposed reach highly accurate by solely using a printed target pattern, which verifies approach.
In this paper, we propose a method by means of light field imaging under structured illumination to deal with high dynamic range 3D imaging. Fringe patterns are projected onto scene and modulated the depth then is detected using recording devices. The contains information about ray direction phase-encoded depth, via which can be estimated from different directions. multidirectional estimation achieve effectively. We analyzed derived phase-depth mapping in proposed flexible ray-based...
Three-dimensional (3D) imaging and metrology of microstructures is a critical task for the design, fabrication, inspection microelements. Newly developed fringe projection 3D microscopy presented in this paper. The system configured according to camera-projector layout long working distance lenses. Scheimpflug principle employed make full use limited depth field. For such specific system, general model introduced reach reconstruction. A dedicated calibration procedure realize quantitative...
Optical manipulation of various kinds nanoparticles is vital in biomedical engineering. However, classical optical approaches demand higher laser power and are constrained by diffraction limits, necessitating tailored trapping schemes for specific nanoparticles. They lack a universal biocompatible tool to manipulate diverse sizes, charges, materials. Through precise modulation diffusiophoresis thermo-osmotic flows the boundary layer an optothermal-responsive gold film, highly adaptable...
A laser beam with circular polarization can be converted into either radial or azimuthal by a microfabricated spiral phase plate and (or azimuthal)-type linear analyzer. The resulting is axially symmetric able to produce tightly focused light fields beyond the diffraction limit. We describe in detail theory behind technique experimental verification of both far field at focus high numerical aperture lens. Vector properties under strong focusing conditions were observed comparing fluorescence...
We demonstrate the proof-of-concept for surface plasmon resonance sensing and imaging via a virtual probe at cell-substrate interface of biological cell in aqueous media. The technique is based on optical excitation by focused radially polarized beams localized plasmons, which forms metal substrate. intensity distribution back focal plane objective lens enables quantitative measurements to be made contact. acquired data then visualized form local refractive index map.
This Letter proposes a new strategy of three-dimensional (3D) scanning pipeline to achieve complete 3D digitization complex objects in real scene. consists one-dimensional array optical sensors combined with an automatically controlled turntable. An efficient calibration method for the sensor is presented guarantee accuracy measurement. Furthermore, automatic registration technique also proposed aligning multiple range images taken from array. Experiment results are demonstrate approach.
An increase in mechanical load the heart causes cardiac hypertrophy, either physiologically (heart development, exercise and pregnancy) or pathologically (high blood pressure heart-valve regurgitation). Understanding hypertrophy is critical to comprehending mechanisms of development treatment disease. However, major molecular event that occurs during physiological pathological dynamic process sarcomeric addition, it has not been observed. In this study, a custom-built second harmonic...
Optical microangiography (OMAG) is a powerful optical angio-graphic tool to visualize micro-vascular flow in vivo. Despite numerous demonstrations for the past several years of qualitative relationship between OMAG and flow, no convincing quantitative has been proven. In this paper, we attempt quantitatively correlate signal with flow. Specifically, develop simplified analytical model complex OMAG, suggesting that product number particles an imaging voxel decorrelation OCT (optical coherence...
In previous work, we presented a structured light field (SLF) method combining imaging with illumination to perform multi-view depth measurement. However, the work just accomplishes rather than 3D reconstruction. this paper, propose novel active involving ray calibration and phase mapping, achieve SLF We performed for first time determine each metric spatio-angular parameters, making realize Based on parametric equation, further derived mapping in that spatial coordinates can be directly...
Two major methods for 3D reconstruction in fringe projection profilometry, phase-height mapping and stereovision, have their respective problems: the former has low-flexibility practical application due to system restrictions latter requires time-consuming homogenous points searching. Given these limitations, we propose a phase-3D method developed from back-projection stereovision model achieve flexible high-efficient profilometry. We showed that all dimensional coordinates (X, Y, Z), but...
Tunneling nanotubes (TNTs) are small membranous tubes of 50–1000 nm diameter observed to connect cells in culture. Transfer subcellular organelles through TNTs was vitro and vivo, but the formation significance these structures is not well understood. A polydimethylsiloxane biochip-based coculture model devised constrain TNT orientation explore both TNT-formation TNT-mediated mitochondrial transfer. Two parallel microfluidic channels connected by an array smaller microchannels enabled...
Micro-Electro-Mechanical System (MEMS) scanning is increasingly popular in 3D surface measurement with the merits of compact structure and high frame-rate. In this paper, we achieve real-time fringe structured reconstruction by using a uniaxial MEMS-based projector. To overcome limitations on projector lensless unidirectional projection, novel isophase plane model proposed, which laser line from regarded as an plane. Our directly establishes mapping relationship between phase spatial...
Surface plasmons excited by a focused femtosecond radially polarized beam on metal surface form standing wave pattern with sharp peak that can be used as "virtual probe" for plasmon microscopy. The rotational symmetry of light effectively provides the TM polarization required coupling to while short pulse nature probe allows nonlinear processes studied.
We propose a novel optical asymmetric cryptosystem based on phase-truncated Fourier transform. Two decryption keys independent of each other are generated. They referred to as universal key and special key, respectively. Each them can be used for independently in absence the other. The is applicable decrypt any ciphertext encoded by same encryption but with poor legibility. On contrary, adequate legible decryption, only valid one corresponding specified plaintext. A set simulation results...
Understanding how stem cells interact with cardiomyocytes is crucial for cell-based therapies to restore the cardiomyocyte loss that occurs during myocardial infarction and other cardiac diseases. It has been thought functional repair regeneration could be regulated by cell-cardiomyocyte contact. However, because various contact modes (junction formation, cell fusion, partial tunneling nanotube formation) occur randomly in a conventional coculture system, particular regulation corresponding...
We have designed a laser cell deposition system that employs the phenomenon of guidance to place single cells at specific points in variety vitro environments. Here, we describe components system: optics, chamber, microinjection feeding and our custom control software application. discuss requirements challenges involved how present overcomes these challenges. demonstrate patterning is accurate within one micrometer by repeatedly depositing polymer microspheres measuring their position. its...