Four commercial composts were added to soil study their effect on plant growth, total rhizosphere microflora, and incidence of growth-promoting rhizobacteria (PGPR) in the tomato plants. Three compost treatments significantly improved while one treatment depressed it. Compost amendments caused only small variations numbers bacteria, actinomycetes, fungi A 709 bacteria isolated from four control determine percentage PGPR each treatment. The tests measured antagonism soilborne root pathogens,...

The regulation of cardiac muscle contraction must differ from that skeletal muscles to effect different physiological and contractile properties. Cardiac troponin C (TnC), the key regulator contraction, possesses functional Ca²⁺-binding properties compared with TnC features a site I, which is naturally inactive. structure in Ca²⁺-saturated state has been determined by nuclear magnetic resonance spectroscopy. regulatory domain exists "closed" conformation even...

While calcium binding to troponin C (TnC) triggers the contraction of both skeletal and cardiac muscle, there is clear evidence that different mechanisms may be involved. For example, activation heart myofilaments occurs with a single regulatory site on TnC, whereas fast two sites. The physiological difference between not understood at molecular level due lack structural details for response TnC calcium. We determined solution structures apo calcium-saturated domain human by using...

Abstract The backbone resonance assignments have been completed for the apo ( 1 H and 15 N) calcium‐loaded H, N, 13 C) regulatory N‐domain of chicken skeletal troponin‐C (1–90), using multidimensional homonuclear heteronuclear NMR spectroscopy. chemical‐shift information, along with detailed NOE analysis 3 J HNHα coupling constants, permitted determination quantification Ca 2+ ‐induced secondary structural change in TnC. For both structures, 5 helices 2 short β‐strands were found, as was...

The structural transition in troponin C induced by the binding of two calcium ions involves an "opening" structure, event that triggers skeletal muscle contraction. We have solved solution structure a mutant (E41A) regulatory domain wherein one bidentate ligand to site I is missing. This remains "closed" upon binding, indicating linkage between and conformational change has been broken. provides snapshot off on state thereby valuable insight into mechanism regulation within TnC. Although...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe solution structure of a trisaccharide-antibody complex: comparison NMR measurements with crystal structureDavid R. Bundle, Herbert Baumann, Jean-Robert Brisson, Stephane M. Gagne, Alexander Zdanov, and Miroslaw CyglerCite this: Biochemistry 1994, 33, 17, 5183–5192Publication Date (Print):May 1, 1994Publication History Published online1 May 2002Published inissue 1...

Nuclear magnetic resonance (NMR) is a powerful tool for observing the motion of biomolecules at atomic level. One technique, analysis relaxation dispersion phenomenon, highly suited studying kinetics and thermodynamics biological processes. Built on top relax computational environment NMR dynamics new designed to be comprehensive, accurate easy-to-use. The software supports more models, both numeric analytic, than current solutions. An automated protocol, available scripting driving...

Bogorol A (1), a novel peptide antibiotic active against MRSA and VRE, has been isolated from cultures of marine Bacillus sp. collected in Papua New Guinea. The structure bogorol was elucidated by combination spectroscopic analyses chemical degradation. illustrates new structural template for "cationic antibiotics".

To understand the spinning process of dragline silk by spiders, protein conformation before has to be determined. Raman confocal spectromicroscopy been used study proteins in situ intact abdominal major ampullate gland Nephila clavipes and Araneus diadematus spiders. The spectra obtained are typical natively unfolded very similar that a mixture recombinant proteins. Vibrational circular dichroism reveals is composed random polyproline II (PPII) segments with some α-helices. α-helices seem...

Spider silk proteins undergo a complex series of molecular events before being converted into an outstanding hierarchically organized fiber. Recent literature has underlined the crucial role C-terminal domain in protein stability and fiber formation. However, effect pH remains to be clarified. We have thus developed efficient purification protocol obtain stable native-like recombinant MaSp1 Nephila clavipes (NCCTD). Its structure was investigated as function using circular dichroism,...

Abstract Background Glioblastoma is an aggressive brain cancer with a 5-year survival rate of 5-10%. Current therapeutic options are limited, due in part to drug exclusion by the blood-brain barrier, restricting access targeted drugs tumor. The receptor for type 1 insulin-like growth factor (IGF-1R) was identified as target glioblastoma. We previously reported that intracerebral glioma cells reduced IGF-1R levels inhibited. objectives this study were evaluate sensitivity novel IGF-axis...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCalcium Binding to the Regulatory N-Domain of Skeletal Muscle Troponin C Occurs in a Stepwise MannerMonica X. Li, Stephane M. Gagne, Sakae Tsuda, Cyril Kay, Lawrence B. Smillie, and Brian D. SykesCite this: Biochemistry 1995, 34, 26, 8330–8340Publication Date (Print):July 4, 1995Publication History Published online1 May 2002Published inissue 4 July 1995https://doi.org/10.1021/bi00026a014Request reuse permissionsArticle...

Rat parvalbumin (PV), an EF-hand type Ca(2+)-binding protein, was expressed in Escherichia coli and mutated by replacing a Phe at position 102 with unique Trp order to introduce distinct fluorescent label into the protein. Mass spectroscopy NMR data indicate that recombinant wild-type (PVWT) F102W mutant (PVF102W) proteins have expected molecular weight retain native structure. Both contain two non-cooperative Ca2+/Mg(2+)-binding sites intrinsic affinity constants, KCa KMg, of 2.4 +/- 0.9 x...

Backbone dynamics of TEM-1 beta-lactamase (263 amino acids, 28.9 kDa) were studied by 15N nuclear magnetic resonance relaxation at 11.7, 14.1, and 18.8 T. The high quality the spectra allowed us to measure longitudinal rate (R1), transverse (R2), {1H}-15N NOE for up 227 250 potentially observable backbone amide groups. model-free formalism was used determine internal motional parameters using an axially anisotropic model. exhibits a small prolate axial anisotropy...

Papaya mosaic potexvirus (PapMV) coat protein (CP) was expressed (CPΔN5) in Escherichia coli and showed to self assemble into nucleocapsid like particles (NLPs). Twenty per cent of the purified found as NLPs 50 nm length 80% a multimer 450 kDa (20 subunits) arranged disk. Two mutants RNA binding domain PapMV CP, K97A E128A interesting properties. The proteins both could be easily CD spectra these secondary tertiary structures similar WT protein. mutant unable bind RNA. On contrary, an...

Apolipophorin III (apoLp-III) from the insect<i>Manduca sexta</i> is a 166-residue (<i>M</i> _r18,340) member of exchangeable apolipoprotein class that functions to stabilize lipid-enriched plasma lipoproteins. In present study, we secondary structure and global fold recombinant apoLp-III derived three-dimensional heteronuclear NMR spectroscopy experiments. Five discrete α-helical segments (21–30 residues in length) with well defined boundaries were characterized by four...

Ca2+ binding to the N-domain of skeletal muscle troponin C (sNTnC) induces an "opening" structure [Gagné, S. M., et al. (1995) Nat. Struct. Biol. 2, 784−789], which is typical Ca2+-regulatory proteins. However, recent structures E41A mutant (E41A sNTnC) (1997) Biochemistry 36, 4386−4392] and cardiac (cNTnC) [Sia, K., J. Chem. 272, 18216−18221] reveal that both these proteins remain essentially in "closed" conformation their Ca2+-saturated states. Both are modified Ca2+-binding site I, albeit...

To evaluate the capacity of Oenococcus oeni strains to release aroma compounds from glycosylated precursors by measuring glycosidase activities with both synthetic and natural substrates.Five were investigated in 47 O. using substrates. This screening revealed that activity levels vary considerably, not only for each strain (depending on substrate tested), but also between strains. Fifteen exhibiting different profiles further analysed substrates extracted untoasted toasted oak. In latter,...