Heat shock protein family A (HSP70) member 5 (HSPA5) is aberrantly expressed in various tumors and closely associated with the progression prognosis of cancer. Nevertheless, its role bladder cancer (BCa) remains elusive. The results our study demonstrated that HSPA5 was upregulated BCa correlated patient prognosis. Cell lines low expression level were constructed to explore this BCa. knockdown promoted apoptosis retarded proliferation, migration invasion cells by regulating VEGFA/VEGFR2...

Accumulating evidences have indicated that aberrant expression of long non-coding RNAs (LncRNAs) is tightly associated with cancer development. Previous studies reported lncRNA XIST regulates tumor malignancies in several cancers. However, the underlying mechanism prostate remains unclear. In current study, we found was down-regulated specimens and cell lines. Low correlated poor prognosis advanced stage patients. gain loss function assays, confirmed suppressed cellular proliferation...

Long-term outcomes after acute kidney injury (AKI) include incremental loss of function and progression towards chronic disease (CKD); however, the pathogenesis AKI to CKD remains largely unknown. Clusterin (CLU) is a chaperone-like protein that reduces ischemia-reperfusion (IRI) enhances tissue repair IRI in kidney. This study investigated role CLU transition renal fibrosis. was induced left kidneys wild type (WT) C57BL/6J (B6) versus knockout (KO) B6 mice by clamping pedicles for 28 min at...

The pathogenetic mechanisms underlying ischemia-reperfusion (I/R) injury involve oxidative stress, inflammation and apoptosis. Nesfatin-1, a novel peptide, has been reported to possess antioxidant, anti-inflammatory anti-apoptic properties. study was examine the potential protective effects of nesfatin-1 on renal I/R injury.I/R model induced by placing clamp across left artery for 45 min followed 24 h reperfusion, along with contralateral nephrectom. Twenty-four rats divided into three...

Prostate cancer (PCa) is a malignant tumor with high incidence in males. Localized tumors can be treated via surgery or radiation; however, it remains difficult to prevent disease progression. Bromodomain-containing protein 4 (BRD4) an epigenetic reader that binds acetylated lysine on histones and has been reported serve critical roles numerous types of cancers. In the present study, was demonstrated BRD4 expression levels were significantly increased cancerous prostate tissue specimens...

Elucidation of the downstream targets regulated by metastasis-suppressive miRNAs can shed light on metastatic processes in prostate cancer (PCa). We conducted microarray analyses and found that miR-195 was significantly decreased PCa. Low expression is an independent prognostic factor for poor biochemical recurrence-free overall survival. Forced PCa cells drastically inhibits proliferation, migration invasion vitro tumor growth metastasis vivo. BCOX1 identified as a direct target PCa, to be...

The aim of the current study was to investigate role enhancer zeste 2 polycomb repressive complex subunit (EZH2) in progression bladder cancer. Human cancer tissue samples were analyzed by immunohistochemistry, and association between clinicopathological parameters EZH2 expression analyzed. proliferation, apoptosis migration ability human cell lines E-J 5637 with or without inhibitor UNC1999 investigated. effect further explored a xenograft model nude mice. vivo vitro levels EZH2, janus...

Sialic acids are widely distributed in animal tissues, and aberrantly expressed a variety of cancer types. High expression sialic acid contributes to tumor aggressiveness by promoting cell proliferation, migration, angiogenesis, metastasis. Sialidases responsible for removal from glycoproteins glycolipids.N-glycomics bladder cells were detected MALDI-TOF mass spectrometry. modification tissue was determined lectin blot. The down-regulation NEU1 high resolution liquid chromatography...

Purpose The purpose of this study is to assess the potential effects metformin on development EMT and tubulointerstitial fibrosis 12 weeks after acute renal ischemia–reperfusion. Methods Male Sprague–Dawley rats were randomly assigned four groups: Sham, IRI, transient administration (TAM), continuous (CAM). Metformin was administered i.p. at a dose 125 μg kg − 1 d 3 prior suffering from IRI or before reperfusion Renal function, histology, expressions IL-6, TNF-α, α-SMA, TGF-β1, Vimentin,...

Abstract Prostate cancer (PCa), the second most mortal from developed countries, presents a high level of chemoresistance. There is emerging evidence underscores critical role autophagy in onset, progression, and chemoresistance PCa. In present study, we investigated possible novel regulator, activating molecule beclin1-regulated autophagy1 (Ambra1), ATG gene sensitivity or PCa cells to cisplatin. We explored regulation by Ambra1 manipulation on induction apoptosis human DU145 presence...

NCOA7 is a nuclear receptor coactivator that downregulated in variety of cancers. However, the expression and prognostic significance clear cell renal carcinoma (ccRCC) remain unknown. The ccRCC tissues was analyzed using bioinformatics analysis, Western blotting, immunohistochemistry. Kaplan–Meier receiver operating characteristic (ROC) curve, clinicopathological correlation analysis were used to assess predictive power NCOA7. Overexpression function tests conducted cells mouse models...

Background: Renal cell carcinoma (RCC) is a common malignancy with high morbidity.MicroRNAs (miRNAs) have been demonstrated to be critical post-transcriptional regulators in tumorigenesis.This study aimed investigate the effect of miR-543 on proliferation and metastasis RCC.Material Methods: The expression was examined clinical samples RCC lines.A498 786-O lines were employed transfected inhibitor or mimics.The correlation between DKK-1 determined by luciferase reporter assay.Cell viability...

// Ruxing Xi 1 , Shupei Pan Xin Chen 2 Beina Hui Li Zhang Shenbo Fu Xiaolong 3 Xuanwei Tuotuo Gong Jia Guo Xiaozhi Shaomin Che Department of Radiotherapy, The First Hospital Affiliated Xi'an Jiao Tong University, Xi'an, Shaan Xi, 710061, P.R.China People's Shaanxi Province, 710068, Liberation Army 323 Hospital, 710054, Correspondence to: Zhang, email: zhang9149@sina.com Che, chersm@126.com Keywords: esophageal squamous cell carcinoma, HPV16 E6-E7, CSCs, PI3K, p75NTR Received: October 22,...

Abstract Background Prostate cancer (PCa) is the second leading cause of cancer-related male deaths worldwide. The purpose this study was to investigate effects homo sapiens solute carrier family 4 member (SLC4A4), which encodes electrogenic Na + /HCO 3 − cotransporter isoform 1 (NBCe1), in development and progression PCa. Methods expression levels SLC4A4 PCa normal prostate tissues were evaluated by immunohistochemistry. knockdown cell model structured lentiviral infection, efficiency...

PTEN pseudogene (PTENP1) has a tumor suppressive role in multiple cancers. However, its involvement esophageal squamous cell carcinoma (ESCC) remains largely unknown. In this study, we set out to identify the of PTENP1 development ESCC. Gene Expression Omnibus database was employed investigate expression sRNA target Database (StarBase v2.0) used query downstream PTENP1. Next, both vitro and vivo experiments were explore function. Cell proliferation evaluated by CCK‐8, soft agar, colony...

Prostate cancer (PCa) is one of the most common cancers and second leading cause mortality in men. Studies exploring novel therapeutic methods are urgently needed. FAM107A, a coding gene located short arm chromosome3, generally downregulated PCa associated with poor prognosis. However, downregulation FAM107A mechanism its action remain challenging to determine. This investigation found that expression was caused by hypermethylation CpG islands. Furthermore, DNA methyltransferase 1 (DNMT1)...

Prostate cancer (PCa) is a malignancy with high incidence and the principal cause of deaths in men. GATA binding protein 5 (GATA5) belongs to gene family. GATA5 has close association carcinogenesis, but role PCa remains poorly understood. The aim our present study was probe into effect on progression elucidate involved mechanism.The expression detected both samples cell lines. overexpression, PLAGL2 knockdown, overexpression models were generated, then Western blotting experiments utilized...

Clear cell renal carcinoma (ccRCC, or KIRC) is the most common type of kidney cancer, originating within cortex. The current outcomes for early diagnosis and late treatment ccRCC are unsatisfactory. Therefore, it important to explore tumor biomarkers therapeutic opportunities ccRCC. In this study, we used bioinformatics methods systematically evaluate expression prognostic value Netrin family genes in Through our analysis, three potential were identified, namely NTNG1, NTNG2, NTN4. Moreover,...

Tacrolimus is a potent immunosuppressant, but has various side effects, with nephrotoxicity being the most common. Renal fibrosis an important process of tacrolimus nephrotoxicity. Therefore, it to identify factors that contribute renal after nephrotoxicity, and control its development.The present study aims determine whether may speed up course by upregulating noncoding RNA activated DNA damage (NORAD) compete miR-136-5p, activating TGF-β1/Smad3 pathway. Furthermore, in vivo rat models...

Pleomorphic adenoma gene like-2 (PLAGL2) is a member of the PLAG family. Previous studies have revealed that overexpression PLAGL2 associated with many human cancers. However, it has been reported also plays as tumor suppressor. The precise role in prostate cancer (PCa) still unknown. aim this study was to investigate expression and prognostic value PCa. Data from microarray datasets demonstrated DNA copy number mRNA level were significantly increased PCa compared normal prostate. qRT-PCR...

Resistance to radiotherapy results in a high treatment failure rate for locally advanced esophageal squamous cell carcinoma (ESCC). Ubiquitin-like with plant homeodomain and ring-finger domains 1 (UHRF1), is associated poor prognosis ESCC. The present study aims characterize the effect of UHRF1 silencing on radiosensitivity ESCC its potential mechanism.Both vitro vivo experiments were conducted observe effects apoptosis cells assessed by flow cytometry. expression apoptosis-related factors...