A5102804343- SARS-CoV-2 detection and testing
- Advanced biosensing and bioanalysis techniques
- Viral gastroenteritis research and epidemiology
- Biosensors and Analytical Detection
- Respiratory viral infections research
- Educational and Organizational Development
- Immune Cell Function and Interaction
- Animal Virus Infections Studies
- Bacteriophages and microbial interactions
- Molecular Biology Techniques and Applications
- SARS-CoV-2 and COVID-19 Research
- Business, Innovation, and Economy
Universidad de Santiago de Chile
2020-2022
Abstract Due to the COVID‐19 pandemic, many transport kits have been manufactured preserve and nasopharyngeal swab samples (NPSs) from patients. However, there is no information on performance of different virus media (VTM) used in diagnosis population Santiago de Chile. We compared RT‐qPCR amplification profile five viral kit mediums, including DNA/RNA Shield™, NAT, VTM‐N, Ezmedlab™, phosphate‐buffered saline (PBS), for NPSs Central Metropolitan Health Service, Santiago, The Shield™ medium...
The early detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using the real-time quantitative polymerase chain reaction (RT-qPCR) as a gold-standard molecular tool has allowed to test and trace viral spread isolation COVID-19-infected patients. capacity internal genes is an essential parameter consider analyze during assay. In this study, we performance two commercial RT-qPCR kits used in Chile, TaqMan™ 2019-nCoV Control Kit v1 (Thermo Fisher) MaxCov19 (TAAG Genetics),...
Abstract The ISAV has a genome composed of eight segments (–)ssRNA, segment 6 codes for the hemagglutinin–esterase protein, and most variable region genome, highly polymorphic (HPR), which is unique among orthomyxoviruses. HPR been associated with virulence, infectivity pathogenicity. full length called HPR0 strain this avirulent, in contrast to strains deleted that are virulent varying degrees. molecular mechanism gives rise different HPRs remains unclear. Here, we studied vitro evolution...
Introduction The COVID-19 pandemic is still in force, causing global public health challenges and threats. Although vaccination herd immunity have proven to be the most efficient way control pandemic, massive early testing of patients using RT-qPCR technique crucial for constant genomic surveillance. appearance variants SARS-CoV-2 with new mutations can reduce efficiency diagnostic detection. In this sense, several commercial kits been target extensive analysis because low assay performance...
ABSTRACT The COVID-19 pandemic has generated a huge challenge and threat to public health throughout the world population. Reverse transcription associated with real-time Polymerase Chain Reaction (RT-qPCR) been gold-standard molecular tool for diagnosis detection of SARS-CoV-2. Currently, it is used as main strategy testing, traceability, control positive cases For this reason, on-top high demand reagents produced stock-out on several occasions only alternative keep population use different...
ABSTRACT The high demand for supplies during the COVID19-pandemic has generated several stock-out of material and essential reagents needed to meet current diagnosis in worldwide population. In this way, there is limited information regarding performance different virus transport medium (VTM) nasopharyngeal swab sampling (NPS) aimed SARS-CoV-2 detection. We compared RT-qPCR amplification profile four commercial kits, including DNA/RNA Shield™, NAT™, VTM, Phosphate-buffered saline (PBS)...
The implementation of educational activities for Small Medium Micro Enterprises (SMMEs), by Chilean government entities, has been a tradition in the last decade, especially entities aimed at strengthening productive development. Innovation is recognized as crucial process growth, sustainability and competitiveness SMMEs, scenario which formation human capital essential. In times pandemic, it essential to meet dynamic needs company context globalization, domain effective virtual management...