A5013435681- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Selenium in Biological Systems
- Advanced biosensing and bioanalysis techniques
- Bacterial Genetics and Biotechnology
- Respiratory viral infections research
- Chromium effects and bioremediation
- Salmonella and Campylobacter epidemiology
- Trace Elements in Health
- Biosensors and Analytical Detection
- Nanoparticles: synthesis and applications
- Viral gastroenteritis research and epidemiology
- Heavy Metal Exposure and Toxicity
- Nanopore and Nanochannel Transport Studies
- Advanced Nanomaterials in Catalysis
- Immune responses and vaccinations
- Heme Oxygenase-1 and Carbon Monoxide
- Long-Term Effects of COVID-19
- Extraction and Separation Processes
- COVID-19 epidemiological studies
- Molecular Biology Techniques and Applications
- Quantum Dots Synthesis And Properties
- Electrochemical Analysis and Applications
- Arsenic contamination and mitigation
Universidad de Santiago de Chile
2017-2024
Universidad Andrés Bello
2013-2015
Timely detection of severe acute respiratory syndrome due to coronavirus 2 (SARS-CoV-2) by reverse transcription quantitative polymerase chain reaction (RT-qPCR) has been the gold- strategy for identifying positive cases during current pandemic. However, faster and less expensive methodologies are also applied massive diagnosis COVID-19. In this way, rapid antigen test (RAT) is widely used. it necessary evaluate its efficiency considering pandemic context with circulation new viral variants....
Abstract The metalloid tellurite is highly toxic to microorganisms. Several mechanisms of action have been proposed, including thiol depletion and generation hydrogen peroxide superoxide, but none them can fully explain its toxicity. Here we use a combination directed evolution chemical biochemical approaches demonstrate that inhibits heme biosynthesis, leading the accumulation intermediates this pathway hydroxyl radical. Unexpectedly, development resistance accompanied by increased...
Abstract Background Hydrogen peroxide (H 2 O ) is a reactive oxygen species (ROS), which part of the oxidative burst encountered upon internalization Salmonella enterica serovar Typhimurium ( S . Typhimurium) by phagocytic cells. It has previously been established that, ArcAB two-component system plays critical role in ROS resistance, but genes regulated remained undetermined to date. We therefore investigated ArcA regulon aerobically growing before and after exposure H querying gene...
Salmonella Typhimurium is the etiological agent of gastroenteritis in humans and enteric fever mice. Inside these hosts, must overcome hostile conditions to develop a successful infection, process which levels porins may be critical. Herein, role porin OmpD infection was assessed for adherence, invasion proliferation RAW264.7 mouse macrophages BALB/c In cultured macrophages, ΔompD strain exhibited increased phenotypes as compared its parental strain. contrast, overexpression ompD caused...
Abstract Background There is an emerging field to put into practice new strategies for developing molecules with antimicrobial properties. In this line, several metals and metalloids are currently being used these purposes, although their cellular effect(s) or target(s) in a particular organism still unknown. Here we aimed investigate analyze Au 3+ toxicity through combination of biochemical molecular approaches. Results We found that triggers major oxidative unbalance Escherichia coli ,...
The variant of concern (VOC) SARS-CoV-2 Omicron (B.1.1529) has been described as a highly contagious but less virulent than the current being monitored (VBM) Delta (B.1.617.2), causing fewer cases hospitalizations, symptomatology, and deaths associated with COVID-19 disease. Although epidemiological comparison both variants previously reported in other countries, no report indicates their behavior severity infection Chile. In this work, we for first time effect cohort 588 patients from...
Abstract Due to the COVID‐19 pandemic, many transport kits have been manufactured preserve and nasopharyngeal swab samples (NPSs) from patients. However, there is no information on performance of different virus media (VTM) used in diagnosis population Santiago de Chile. We compared RT‐qPCR amplification profile five viral kit mediums, including DNA/RNA Shield™, NAT, VTM‐N, Ezmedlab™, phosphate‐buffered saline (PBS), for NPSs Central Metropolitan Health Service, Santiago, The Shield™ medium...
As the SARS-CoV-2 continues to evolve, new variants pose a significant threat by potentially overriding immunity conferred vaccination and natural infection. This scenario can lead an upswing in reinfections, amplified baseline epidemic activity, localized outbreaks. In various global regions, estimates of breakthrough cases associated with currently circulating viral variants, such as Omicron, have been reported. Nonetheless, specific data on reinfection rate Chile still needs be included....
Metal(loid) salts were used to treat infectious diseases in the past due their exceptional biocidal properties at low concentrations. However, mechanism of toxicity has yet be fully elucidated. The production reactive oxygen species (ROS) been linked soft metal(loid)s such as Ag(I), Au(III), As(III), Cd(II), Hg(II), and Te(IV). Nevertheless, few reports have described direct, or ROS-independent, effects some these soft-metal(loid)s on bacteria, including dismantling iron-sulfur clusters...
The early detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using the real-time quantitative polymerase chain reaction (RT-qPCR) as a gold-standard molecular tool has allowed to test and trace viral spread isolation COVID-19-infected patients. capacity internal genes is an essential parameter consider analyze during assay. In this study, we performance two commercial RT-qPCR kits used in Chile, TaqMan™ 2019-nCoV Control Kit v1 (Thermo Fisher) MaxCov19 (TAAG Genetics),...
The COVID-19 pandemic continues to be a concern and keeps global health authorities on alert. RT-PCR technique has been the gold-standard assay for detecting SARS-CoV-2 virus. However, rapid antigen tests (RATs) have widely used increase number of faster more efficiently in population. Nevertheless, appearance new viral variants, with genomic mutations associated greater contagiousness immune evasion, highlights need evaluate sensitivity these RATs. This report evaluates SD Biosensor-Roche,...
Tellurium is a rare metalloid that exerts high toxicity on cells, especially bacteria, partly due to reactive oxygen species (ROS) generation. Moreover, it has also been observed tellurite can target free cell thiols groups (RSH) (i.e. reduced glutathione (GSH)), enhancing the cellular redox imbalance. Additionally, in vitro experiments have suggested several enzymes reduce (IV) its elemental form (0); where RSH present their active sites may be responsible for process. Nevertheless,...
The current COVID-19 pandemic is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Many countries have reported experience of at least two contagion waves, describing associated mortality rates and population behavior. analysis effect this in different localities can provide valuable information on key factors to consider face future massive infectious diseases. This work describes first retrospective comparative study about behavior during second waves Chile from a...
Introduction The COVID-19 pandemic is still in force, causing global public health challenges and threats. Although vaccination herd immunity have proven to be the most efficient way control pandemic, massive early testing of patients using RT-qPCR technique crucial for constant genomic surveillance. appearance variants SARS-CoV-2 with new mutations can reduce efficiency diagnostic detection. In this sense, several commercial kits been target extensive analysis because low assay performance...
BackgroundAs the COVID-19 pandemic persists, infections continue to surge globally. Presently, most effective strategies curb disease and prevent outbreaks involve fostering immunity, promptly identifying positive cases, ensuring their timely isolation. Notably, there are instances where SARS-CoV-2 virus remains infectious even after patients have completed quarantine.ObjectiveUnderstanding viral persistence post-quarantine is crucial as it could account for localized infection outbreaks....
The identification and tracking of SARS‐CoV‐2 infected patients in the general population are essential components global strategy to limit COVID‐19 viral spread, specifically for maintaining traceability suppressing resurgence local outbreaks. Public health programs that include continuous RT‐qPCR testing population, sequencing, genomic surveillance highly contagious forms virus have allowed infections reinfections. This work identified reinfection a homeless person, which occurred 58 days...
Vaccine administration is one of the most efficient ways to control current coronavirus disease 2019 (COVID-19) pandemic. However, appearance severe acute respiratory syndrome 2 (SARS-CoV-2) variants can avoid immunity generated by vaccines. Thus, in patients with a complete vaccine schedule, infection SARS-CoV-2 may cause severe, mild, and asymptomatic manifestations disease. In this case report, we describe for first time clinical symptoms four (three symptomatic; asymptomatic) from...
Herein we report the use of an environmental multimetal(loid)-resistant strain, MF05, to biosynthesize single- or multi-element nanostructures under anaerobic conditions. Inorganic nanostructure synthesis typically requires methodologies and conditions that are harsh environmentally hazardous. Thus, green/eco-friendly procedures desirable, where microorganisms their extracts as bionanofactories is a reliable strategy. First, MF05 was entirely sequenced identified Escherichia coli-related...
The COVID-19 pandemic continues to affect several countries. One of the best ways control its spread is timely identification infected patients for isolation and quarantine. While an episode infection lasts average 8-10 days from onset symptoms, there literature describing long-lasting viral persistence events. Here, we report a case SARS-CoV-2 386 in health worker Santiago de Chile. Our study could be one longest reported RNA sequencing analyses indicated that first positive diagnosis (8...
ABSTRACT The COVID-19 pandemic has generated a huge challenge and threat to public health throughout the world population. Reverse transcription associated with real-time Polymerase Chain Reaction (RT-qPCR) been gold-standard molecular tool for diagnosis detection of SARS-CoV-2. Currently, it is used as main strategy testing, traceability, control positive cases For this reason, on-top high demand reagents produced stock-out on several occasions only alternative keep population use different...
Abstract Metal(loid) salts have been used to treat infectious diseases due their exceptional biocidal properties at low concentrations. However, the mechanism of toxicity has yet be fully elucidated. The production reactive oxygen species (ROS) linked soft metal(loid)s such as Ag(I), Au(III), As(III), Cd(II), Hg(II), and Te(IV). Nevertheless, few reports described direct, or ROS-independent, effects some these soft-metal(loid)s on bacteria, including dismantling iron-sulphur clusters...
ABSTRACT The high demand for supplies during the COVID19-pandemic has generated several stock-out of material and essential reagents needed to meet current diagnosis in worldwide population. In this way, there is limited information regarding performance different virus transport medium (VTM) nasopharyngeal swab sampling (NPS) aimed SARS-CoV-2 detection. We compared RT-qPCR amplification profile four commercial kits, including DNA/RNA Shield™, NAT™, VTM, Phosphate-buffered saline (PBS)...