A5067747192- HIV Research and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Genomics and Chromatin Dynamics
- Immune Cell Function and Interaction
- Histone Deacetylase Inhibitors Research
- Herpesvirus Infections and Treatments
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Cardiomyopathy and Myosin Studies
- DNA Repair Mechanisms
- Congenital heart defects research
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Neurobiology and Insect Physiology Research
- Signaling Pathways in Disease
- Bacterial Genetics and Biotechnology
- Cytomegalovirus and herpesvirus research
- Fungal and yeast genetics research
- Wnt/β-catenin signaling in development and cancer
- Digestive system and related health
- Growth Hormone and Insulin-like Growth Factors
- Plant Molecular Biology Research
- Hepatitis C virus research
- Muscle Physiology and Disorders
- Cancer-related gene regulation
University of South China
2015-2024
Stockholm University
2015-2023
Sichuan Agricultural University
2022
Shanghai First People's Hospital
2019
Hunan Normal University
2013-2018
Hunan Mawangdui Hospital
2018
Hunan Provincial People's Hospital
2018
Discovery Institute
2017
Sanford Burnham Prebys Medical Discovery Institute
2013-2017
Xiamen University
2014
The third variable region (V3) of the HIV-1 gp120 envelope glycoprotein is immunodominant and contains features essential for coreceptor binding. We determined structure V3 in context an core complexed to CD4 receptor X5 antibody at 3.5 angstrom resolution. Binding cell-surface would position so that its coreceptor-binding tip protrudes 30 angstroms from toward target cell membrane. extended nature accessibility explain immunodominance. Together, results provide a structural rationale role...
The membrane-proximal region of the ectodomain gp41 envelope glycoprotein human immunodeficiency virus type 1 (HIV-1) is target three five broadly neutralizing anti-HIV-1 antibodies thus far isolated. We have determined crystal structures antigen-binding fragment for one these antibodies, 2F5, in complex with 7-mer, 11-mer, and 17-mer peptides region, at 2.0-, 2.1-, 2.2-A resolutions, respectively. reveal an extended conformation, which stretches over 30 A length. Contacts are made...
The CCR5 co-receptor binds to the HIV-1 gp120 envelope glycoprotein and facilitates entry into cells. Its N terminus is tyrosine-sulfated, as are many antibodies that react with binding site on gp120. We applied nuclear magnetic resonance crystallographic techniques analyze structure of tyrosine-sulfated antibody 412d in complex CD4. conformations regions (alpha-helix) (extended loop) surprisingly different. Nonetheless, a critical sulfotyrosine induces similar structural rearrangements...
The site on HIV-1 gp120 that binds to the CD4 receptor is vulnerable antibodies. However, most antibodies interact with this cannot neutralize HIV-1. To understand basis of resistance, we determined co-crystal structures for two poorly neutralizing, CD4-binding (CD4BS) antibodies, F105 and b13, in complexes gp120. Both exhibited approach angles similar those a rare, broadly neutralizing CD4BS antibody, b12. Slight differences recognition, however, resulted substantial F105- b13-bound...
Herpes simplex virus (HSV) glycoproteins E and I (gE gI) can act as a receptor for the Fc domain of immunoglobulin G (IgG). To examine role HSV IgG in viral pathogenesis, rabbits mice were infected by corneal route with gE- or gI- mutants. Wild-type HSV-1 produced large dendritic lesions epithelium subsequent stromal disease leading to encephalitis, whereas mutant viruses microscopic punctate small no encephalitis. These differences not related ability gE-gI oligomer bind because observed...
During human immunodeficiency virus type 1 (HIV-1) infection, patients develop various levels of neutralizing antibody (NAb) responses. In some cases, patient sera can potently neutralize diverse strains HIV-1, but the specificities that mediate this broad neutralization are not known, and their elucidation remains a formidable challenge. Due to variable nonneutralizing determinants on exterior envelope glycoprotein (Env), nonnative Env protein released from cells, glycan shielding assembles...
We recently described the isolation and structural characterization of 2'-fluoropyrimidine-substituted RNA aptamers that bind to gp120 R5 strains human immunodeficiency virus type 1 thereby potently neutralize infectivity phylogenetically diverse strains. Here we investigate physical basis their antiviral action. show both N-linked oligosaccharides variable loops V1/V2 V3 are not required for binding one aptamer, B40, gp120. Using surface plasmon resonance analyses, aptamer binds...
The effect of segregated early weaning (SEW) on postweaning small intestinal development was investigated in SEW and control (CON) pigs. Small intestines were collected from a total 15 pigs killed at 11 (preweaning), (3 d postweaning), 34 age. At 3 postweaning, the CON had shorter villi (P < .01), deeper crypts reduced .01) ratios villus height:crypt depth (V:C) compared with preweaning. Weaning also specific activities lactase duodenum ileum alkaline phosphatase (ALP) .05) jejunum. Sucrase...
Cardiomyopathy is a common disease of cardiac muscle that negatively affects function. HDAC3 commonly functions as corepressor by removing acetyl moieties from histone tails. However, deacetylase-independent role has also been described. Cardiac deletion causes reduced contractility accompanied lipid accumulation, but the molecular function in cardiomyopathy remains unknown. We have used powerful genetic tools Drosophila to investigate enzymatic and nonenzymatic roles cardiomyopathy. Using...
Positive Transcription Elongation Factor b (P-TEFb) is a kinase consisting of Cdk9 and Cyclin T that releases RNA Polymerase II (Pol II) into active elongation. It can assemble larger Super Complex (SEC) additional elongation factors. Here, we use miRNA-based approach to knock down the maternal contribution P-TEFb SEC components in early Drosophila embryos. or depletion results loss cells from embryo posterior cellularization defects. Interestingly, expression many patterning genes...
Abstract Cardiac valves serve an important function; they support unidirectional blood flow and prevent regurgitation. Wnt signaling plays role in the formation of mouse cardiac valve proliferation Zebrafish, but identification specific components involved has not been addressed systematically. Of signal transduction, pygopus ( pygo ), first identified as a core component Drosophila , yet to be investigated with respect development differentiation. Here, we take advantage heart model study...
Understanding the cellular-molecular substrates of heart disease is key to development cardiac specific therapies and prevention off-target effects by non-cardiac targeted drugs. One primary targets for therapeutic intervention has been human ether a go-go (hERG) K+ channel that, together with KCNQ channel, controls rate efficiency repolarization in myocardial cells. Neither these channels plays major role adult mouse function; however, we show here that hERG homolog seizure (sei), along...
ABSTRACT Coxsackievirus B3 (CVB3) is the most common pathogen that induces acute and chronic viral myocarditis in children. The cytopathic effect (CPE)-based neutralization test (Nt-CPE) plaque reduction (PRNT) are methods for measuring neutralizing antibody titers against CVB3 blood serum samples. However, these two inefficient vaccine clinical trials, which require testing of a large number specimens. In this study, we developed an efficient based on enzyme-linked immunospot (Nt-ELISPOT)...
Heart function declines with age, but the genetic factors underlying such deterioration are largely unknown. Wnt signaling is known to play a role in heart development, it has not been shown be important adult function. We have investigated nuclear adapter protein encoded by pygopus (pygo), which mediates canonical signaling, for roles aging-related cardiac dysfunction.
Cell division properties of Escherichia coli B/r containing either a dnaC or dnaI mutation were examined. Incubation at nonpermissive temperature resulted in the eventual production cells approximately normal size, slightly smaller, which lacked chromosomal DNA. The cell patterns cultures grown permissive and then shifted to consistent with: first, equipartition chromosomes by C D periods time shift; second, an apparent delay division; third, commencement formation chromosomeless cells. In...
ABSTRACT The histone deacetylase HDAC3 is associated with the NCoR/SMRT co-repressor complex, and its canonical function in transcriptional repression, but it can also activate transcription. Here, we show that repressor activator functions of be genetically separated Drosophila. A lysine substitution N terminus (K26A) disrupts catalytic activity function, whereas a combination substitutions (HEBI) abrogating interaction SMRTER enhances beyond wild type early embryo. We conclude crucial...