A5037164659- Transgenic Plants and Applications
- Plant tissue culture and regeneration
- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- Pineapple and bromelain studies
- Toxin Mechanisms and Immunotoxins
- Phytase and its Applications
- Research in Cotton Cultivation
- Monoclonal and Polyclonal Antibodies Research
- Plant-Microbe Interactions and Immunity
- Viral gastroenteritis research and epidemiology
- Genetically Modified Organisms Research
- Microbial Inactivation Methods
- Plant Reproductive Biology
- Enzyme Production and Characterization
- Insect Resistance and Genetics
- Microbial Metabolites in Food Biotechnology
- Plant Micronutrient Interactions and Effects
- Animal Genetics and Reproduction
- Plant Pathogens and Fungal Diseases
- Animal Disease Management and Epidemiology
- Iron Metabolism and Disorders
- Mycorrhizal Fungi and Plant Interactions
Protein Express (United States)
2021
Texas A&M Health Science Center
2018
Caliber Imaging and Diagnostics (United States)
2013-2015
Texas A&M University
2011-2012
BOKU University
2006-2010
University of Lausanne
2010
RWTH Aachen University
2004-2010
Consejo Superior de Investigaciones Científicas
2006
Centro de Investigaciones Biológicas Margarita Salas
2006
Universitat de Lleida
2006
Abstract Magnaporthe oryzae causes rice blast, the most serious foliar fungal disease of cultivated (Oryza sativa). During hemibiotrophic leaf infection, pathogen simultaneously combines biotrophic and necrotrophic growth. Here, we provide cytological molecular evidence that, in contrast to tissue fungus adopts a uniquely infection strategy roots for prolonged period spreads without causing loss host cell viability. Consistent with lifestyle, intracellularly growing hyphae M. are surrounded...
Plant-based biomanufacturing of therapeutic proteins is a relatively new platform with small number commercial-scale facilities, but offers advantages linear scalability, reduced upstream complexity, time to market, and potentially lower capital operating costs. In this study we present detailed process simulation model for large-scale "greenfield" facility that uses transient agroinfiltration Nicotiana benthamiana plants grown hydroponically indoors under light-emitting diode lighting the...
Protein transport within cereal endosperm cells is complicated by the abundance of endoplasmic reticulum (ER)-derived and vacuolar protein bodies. For wheat storage proteins, two major routes run from ER to vacuole, one bypassing passing through Golgi. Proteins traveling along each route converge at vacuole form aggregates. To determine impact this trafficking system on fate recombinant proteins expressed in endosperm, we used confocal electron microscopy investigate three containing...
Recombinant proteins directed to the secretory pathway in plants require a signal peptide for entry into endoplasmic reticulum. In absence of further targeting information, such are generally secreted via default apoplast. This has been well documented protoplasts and leaf tissue, but trafficking recombinant seeds other storage tissues rarely investigated. We used Aspergillus niger phytase as model glycoprotein compare intracellular fate protein leaves rice (Oryza sativa). Using fluorescence...
Summary Cottonseed remains a low‐value by‐product of lint production mainly due to the presence toxic gossypol that makes it unfit for monogastrics. Ultra‐low cottonseed (ULGCS) lines were developed using RNAi knockdown δ‐cadinene synthase gene(s) in Gossypium hirsutum . The purpose current study was assess stability and specificity ULGCS trait evaluate agronomic performance transgenic lines. Trials conducted over period 3 years show stable under field conditions foliage/floral organs...
Summary Cottonseed, containing 22.5% protein, remains an under‐utilized and under‐valued resource because of the presence toxic gossypol. RNAi‐knockdown δ‐cadinene synthase gene(s) was used to engineer plants that produced ultra‐low gossypol cottonseed (ULGCS). In original study, we observed RNAi plants, a month or older, maintain normal complement related terpenoids in roots, foliage, floral organs, young bolls. However, terpenoid levels profile lines during early stages germination, under...
Abstract Zeins are the major storage proteins in maize (Zea mays) endosperm, and their accumulation zein bodies derived from endoplasmic reticulum is well characterized. In contrast, relatively little known about post-Golgi compartments or trafficking of vacuolar specifically presence globulins structures resembling protein vacuoles that appear early to mid-stage seed development. We investigated this pathway by expressing analyzing a recombinant reporter glycoprotein during endosperm...
Classical Swine Fever Virus (CSFV) causes classical swine fever, a highly contagious hemorrhagic fever affecting both feral and domesticated pigs. Outbreaks of CSF in Europe, Asia, Africa South America had significant adverse impacts on animal health, food security the pig industry. The disease is generally contained by prevention exposure through import restrictions (e.g. banning live pigs pork products), localized vaccination programmes culling infected or at-risk animals, often at very...
N-glycosylation has been shown to affect the pharmacokinetic properties of several classes biologics, including monoclonal antibodies, blood factors, and lysosomal enzymes. In last two decades, N-glycan engineering employed achieve a profile that is either more consistent or aligned with specific improved activity (i.e., effector function serum half-life). particular, attention focused on processes in vivo vitro alter structure Fc region anti-cancer antibodies order increase...
Abstract The encapsulation of biopharmaceuticals into micro‐ or nanoparticles is a strategy frequently used to prevent degradation achieve the slow release therapeutics and vaccines. Protein bodies (PBs), which occur naturally as storage organelles in seeds, can be such carrier vehicles. fusion N‐terminal sequence maize protein, γ‐zein, other proteins sufficient induce formation PBs, bioencapsulate recombinant directly plant production host. In addition, immunostimulatory effects zein have...
N-glycosylation profoundly affects the biological stability and function of therapeutic proteins, which explains recent interest in glycoengineering technologies as methods to develop biobetter therapeutics. In current manufacturing processes, is host-specific remains difficult control a production environment that changes with scale batches leading glycosylation heterogeneity inconsistency. On other hand, vitro chemoenzymatic glycan remodeling has been successful producing homogeneous...
N-glycosylation has been shown to affect the pharmacokinetic properties of several classes biologics including monoclonal antibodies, blood factors, and lysosomal enzymes. In last two decades, N-glycan engineering employed achieve a profile that is either more consistent or aligned with specific improved activity (i.e. effector function serum half-life). particular, attention focused on processes in vivo vitro alter structure Fc region anti-cancer antibodies order increase antibody-dependent...