A5068257270- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Viral Infections and Immunology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Viral gastroenteritis research and epidemiology
- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- Animal Virus Infections Studies
- RNA and protein synthesis mechanisms
- T-cell and Retrovirus Studies
- Estrogen and related hormone effects
- Legume Nitrogen Fixing Symbiosis
- DNA and Nucleic Acid Chemistry
- Lipid Membrane Structure and Behavior
- bioluminescence and chemiluminescence research
- RNA Interference and Gene Delivery
- Bioactive Compounds and Antitumor Agents
- Food Chemistry and Fat Analysis
- Animal Nutrition and Physiology
- Toxin Mechanisms and Immunotoxins
- Proteins in Food Systems
- Genomics and Chromatin Dynamics
- Advanced Biosensing Techniques and Applications
- CRISPR and Genetic Engineering
- Polymer Surface Interaction Studies
Plum Island Animal Disease Center
2011-2023
Cook Medical (Ireland)
2020-2022
United States Department of Homeland Security
2020
Leidos (United States)
2018
Rasmussen College
2018
Agricultural Research Service
1993-2004
United States Department of Agriculture
1996-2002
Irish Equine Centre
1993
Ollscoil na Gaillimhe – University of Galway
1984-1989
University College Dublin
1986
An open reading frame, LMW5-HL, in the African swine fever virus genome displays a high degree of similarity to proto-oncogene bcl-2 and, lesser degree, Epstein-Barr gene BHRF1. A highly conserved central region is found all three proteins. LMW5-HL encodes protein 18 kDa that present infected porcine macrophages at both early and late times postinfection. The BHRF1 suggests role for it cell maintenance during productive or persistent viral infection.
African swine fever virus (ASFV) multigene family 360 and 530 (MGF360/530) genes affect viral growth in macrophage cell cultures virulence pigs (L. Zsak, Z. Lu, T. G. Burrage, J. Neilan, F. Kutish, D. M. Moore, L. Rock, Virol. 75:3066-3076, 2001). The mechanism by which these novel virus-host interactions is unknown. To define MGF360/530 gene function, we compared transcriptional responses following infection with parental ASFV (Pr4) an deletion mutant (Pr4 Delta 35). A cDNA microarray...
ABSTRACT A fluorogenic probe hydrolysis (TaqMan) PCR assay for African swine fever virus (ASFV) was developed and evaluated in experimentally infected swine. This sensitive specific one-step single-tube assay, which can be performed 2 h or less, detected viral DNA tonsil scraping samples to 4 days prior onset of clinical disease. Thus, the would have application preclinical diagnosis surveillance and/or emergency management a disease outbreak.
We described previously an African swine fever virus (ASFV) open reading frame, 23-NL, in the isolate Malawi Lil 20/1 whose product shared significant similarity a carboxyl-terminal domain with those of mouse myeloid differentiation primary response gene, MyD116, and herpes simplex neurovirulence-associated ICP34.5 (M. D. Sussman, Z. Lu, G. Kutish, C. L. Afonso, P. Roberts, Rock, J. Virol. 66:5586-5589, 1992). The 23-NL to these genes suggested that this gene may function some aspect ASFV...
ABSTRACT An African swine fever virus (ASFV) gene with similarity to the T-lymphocyte surface antigen CD2 has been found in pathogenic isolate Malawi Lil-20/1 (open reading frame [ORF] 8-DR) and a cell culture-adapted European virus, BA71V (ORF EP402R) shown be responsible for hemadsorption phenomenon observed ASFV-infected cells. The structural functional similarities of ASFV product CD2, cellular protein involved cell-cell adhesion T-cell-mediated immune responses, suggested possible role...
ABSTRACT The African swine fever virus (ASFV) genome contains a gene, 9GL , with similarity to yeast ERV1 and ALR genes. has been shown function in oxidative phosphorylation cell growth, while hepatotrophic activity. encodes protein of 119 amino acids was highly conserved at both nucleotide acid levels among all ASFV field isolates examined. Monospecific rabbit polyclonal antibody produced glutathione S -transferase–9GL fusion specifically immunoprecipitated 14-kDa from macrophage cultures...
African Swine Fever Virus (ASFV) is a high-consequence transboundary animal pathogen that often causes hemorrhagic disease in swine with case fatality rate close to 100%. Lack of treatment or vaccine for the makes it imperative safe and efficacious vaccines are developed safeguard industry. In this study, we evaluated immunogenicity seven adenovirus-vectored novel ASFV antigens, namely A151R, B119L, B602L, EP402RΔPRR, B438L, K205R A104R. Immunization commercial cocktail recombinant...
The African swine fever virus (ASFV) causes a fatal hemorrhagic disease in domestic swine, and at present no treatment or vaccine is available. Natural gene-deleted, live attenuated strains protect against closely related virulent strains; however, they are yet to be deployed evaluated the field rule out chronic persistence potential for reversion virulence. Previous studies suggest that antibodies play role protection, but induction of cytotoxic T lymphocytes (CTLs) could key complete...
ABSTRACT Pathogenic African swine fever virus (ASFV) isolates primarily target cells of the mononuclear-phagocytic system in infected and replicate efficiently primary macrophage cell cultures vitro. ASFVs can, however, be adapted to grow monkey lines. Characterization two culture-adapted viruses, MS16 BA71V, revealed that neither replicated cultures. Cell viability experiments ultrastructural analysis showed infection with these viruses resulted early death, which occurred prior viral...
ABSTRACT The presence of a previously unidentified exon upstream the originally described human oestrogen receptor (hOR) gene is demonstrated. This shown to be spliced 5′ untranslated region designated I. resulting genomic structure thus in agreement with mouse OR and highlights conservation an 18 amino acid open-reading frame formed from above splicing event. Taken conjunction previous publications this would suggest that hOR complex transcriptional unit contains two promoters.
ABSTRACT Sequence analysis of the right variable genomic region pathogenic African swine fever virus (ASFV) isolate E70 revealed a novel gene, UK , that is immediately upstream from previously described ASFV virulence-associated gene NL-S (L. Zsak, Z. Lu, G. F. Kutish, J. Neilan, and D. L. Rock, Virol. 70:8865–8871, 1996). transcriptionally oriented toward end genome, predicts protein 96 amino acids with molecular mass 10.7 kDa. Searches genetic databases did not find significant similarity...
Here, we show that the African swine fever virus 5-HL gene is a highly conserved viral and contains all known protein domains associated with Bcl-2 activity, including those involved dimerization, mediating cell death, protein-binding functions, its product, p21, suppresses apoptotic death in mammalian lymphoid line FL5.12. Thus, true functional member of family.
Rabbit Hemorrhagic Disease (RHD) is a severe acute viral disease specifically affecting the European rabbit Oryctolagus cuniculus. As predominant species of domestic throughout world, RHD contributes towards significant losses to farming industries and endangers wild populations rabbits in Europe other predatory animals that depend upon as food source. virus (RHDV) – Lagovirus belonging family Caliciviridae etiological agent RHD. Typically, presents with sudden death 70% 95% infected...
ABSTRACT Recently, we reported that African swine fever virus (ASFV) multigene family (MGF) 360 and 530 genes are significant macrophage host range determinants function by promoting infected-cell survival. To examine the of these in ASFV's arthropod host, Ornithodoros porcinus , an MGF360/530 gene deletion mutant (Pr4Δ35) was constructed from ASFV isolate tick origin, Pr4. Pr4Δ35 exhibited a growth defect ticks. The six MGF360 two MGF530 Pr4 markedly reduced viral replication infected ticks...
The results of the first decade development a replication-defective human adenovirus serotype 5 (Ad5) containing capsid- and 3C protease-coding regions foot-and-mouth disease (FMD) virus as vaccine candidate are presented. In proof-of-concept studies, it was demonstrated that single inoculation with this vector capsid FMD A24 Cruzeiro protected both swine cattle following homologous challenge by direct 1 week postvaccination. We have expanded these studies in larger numbers animals testing...
ABSTRACT Previously we have shown that the African swine fever virus (ASFV) NL gene deletion mutant E70ΔNL is attenuated in pigs. Our recent observations mutants of two additional pathogenic ASFV isolates, Malawi Lil-20/1 and Pr4, remained highly virulent (100% mortality) suggested these isolates encoded an virulence determinant(s) was absent from E70. To map this putative determinant, vivo marker rescue experiments were performed by inoculating with infection-transfection lysates containing...
ABSTRACT African swine fever virus (ASFV) replicates in the cytoplasm of infected cells and contains genes encoding a number enzymes needed for DNA synthesis, including thymidine kinase (TK) gene. Recombinant TK gene deletion viruses were produced by using two highly pathogenic isolates ASFV through homologous recombination with an p72 promoter–β-glucuronidase indicator cassette (p72GUS) flanked sequences targeting region. Attempts to isolate double-crossover mutants on macrophages failed,...
Classical Swine Fever Virus (CSFV) causes classical swine fever, a highly contagious hemorrhagic fever affecting both feral and domesticated pigs. Outbreaks of CSF in Europe, Asia, Africa South America had significant adverse impacts on animal health, food security the pig industry. The disease is generally contained by prevention exposure through import restrictions (e.g. banning live pigs pork products), localized vaccination programmes culling infected or at-risk animals, often at very...
The safety and efficacy of an experimental, replication-deficient, human adenovirus-vectored foot-and-mouth disease virus (FMDV) serotype A24 Cruzeiro capsid-based subunit vaccine (AdtA24) was examined in eight independent cattle studies. AdtA24 non-adjuvanted administered intramuscularly to a total 150 steers doses ranging from approximately 1.0 × 108 2.1 1011 particle units per animal. No detectable local or systemic reactions were observed after vaccination. At 7 days post-vaccination...
Lipid cubic phase formulations have gained recognition as potential controlled delivery systems for a range of active pharmaceutical and biological agents on account their desirable physiochemical properties ability to encapsulate both hydrophobic hydrophilic molecules. The most widely studied lipid are those the monoacylglycerol family. These susceptible lipolysis by variety enzymes, including lipases esterases, which attack ester bond present chain bridging oleic acid component glycerol...
An African swine fever virus (ASFV) ORF, 8CR, with similarity to the C-type lectin family of adhesion proteins has been described in pathogenic isolate Malawi Lil-20/1. The 8CR cellular and poxvirus genes associated cell adhesion, recognition infectivity suggested that may be significance ASFV-host interactions. Sequence analysis ORF from additional ASFV isolates demonstrated conservation among both pig tick sources. Northern blot mRNA transcription late replication cycle. A Lil-20/1...