A5019136051- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Animal Virus Infections Studies
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
Kansas State University
2017-2023
Texas A&M University
2014-2017
African Swine Fever Virus (ASFV) is a high-consequence transboundary animal pathogen that often causes hemorrhagic disease in swine with case fatality rate close to 100%. Lack of treatment or vaccine for the makes it imperative safe and efficacious vaccines are developed safeguard industry. In this study, we evaluated immunogenicity seven adenovirus-vectored novel ASFV antigens, namely A151R, B119L, B602L, EP402RΔPRR, B438L, K205R A104R. Immunization commercial cocktail recombinant...
The African swine fever virus (ASFV) causes a fatal hemorrhagic disease in domestic swine, and at present no treatment or vaccine is available. Natural gene-deleted, live attenuated strains protect against closely related virulent strains; however, they are yet to be deployed evaluated the field rule out chronic persistence potential for reversion virulence. Previous studies suggest that antibodies play role protection, but induction of cytotoxic T lymphocytes (CTLs) could key complete...
: African swine fever (ASF) is a viral disease of domestic and wild suids for which there currently no vaccine or treatment available. The recent spread ASF virus (ASFV) through Europe Asia causing enormous economic animal losses. Unfortunately, the measures taken so far are insufficient an effective against ASFV needs to be urgently developed. We hypothesized that immunization with cocktail thirty-five rationally selected antigens would improve protective efficacy subunit prototypes given...
African swine fever virus (ASFV) is a pathogen of great economic importance given that continues to threaten the pork industry worldwide, but there no safe vaccine or treatment available. Development feasible as immunization pigs with some live attenuated ASFV candidates can confer protection, safety concerns and scalability are challenges must be addressed. Identification protective antigens needed inform development efficacious subunit vaccines.In this study, replication-incompetent...
African Swine Fever Virus (ASFV) poses a serious threat to the pork industry worldwide; however, there is no safe vaccine or treatment available. The development of an efficacious subunit will require identification protective antigens. ASFV pp220 polyprotein essential for virus structural integrity. This processed generate p5, p34, p14, p37, and p150 individual proteins. Immunization pigs with cocktail adenoviruses expressing proteins induced significant IgG, IFN-γ-secreting cells,...
Bovine Viral Diarrhea Virus (BVDV) is an important pathogen that plays a significant role in initiating Respiratory Disease Complex (BRDC) cattle. The disease causes multi-billion dollar losses globally due to high calf mortality and increased morbidity leading heavy use of antibiotics. Current commercial vaccines provide limited cross-protection with several drawbacks such as safety, immunosuppression, potential reversion virulence, induction neonatal pancytopenia. This study evaluates two...
Bovine viral diarrhea virus (BVDV) plays a key role in bovine respiratory disease complex, which can lead to pneumonia, and death of calves. Current vaccines are not very effective due, part, immunosuppressive traits failure induce broad protection. There diverse BVDV strains thus, current contain representative genotype 1 2 viruses (BVDV-1 & 2) broaden coverage. modified live (MLV) superior killed vaccines, but they susceptible neutralization complement-mediated destruction triggered by...
Lack of safe and effective adjuvants is a major hindrance to the development efficacious vaccines. Signaling via CD40 pathway leads enhanced antigen processing presentation, nitric oxide expression, pro-inflammatory cytokine expression by presenting cells, stimulation B-cells undergo somatic hypermutation, immunoglobulin class switching, proliferation. Agonistic anti-CD40 antibodies have shown promising adjuvant qualities in human mouse vaccine studies. An monoclonal antibody (mAb),...
Abstract Bovine viral diarrhea virus (BVDV), a Flavivirus, plays key role in bovine respiratory disease complex, which leads to pneumonia and death of calves. Current vaccines are not very effective due, part, immunosuppressive traits the virus. There diverse BVDV strains thus, current contain type 1 2 viruses (BVDV-1 & 2). Modified live (MLV) superior killed virus, but they susceptible antibody neutralization complement-mediated destruction that limits their efficacy neonates. We...
Abstract Development of subunit vaccines against complex pathogens entails inclusion multiple targets to improve efficacy and or broaden protection. Importantly, testing an immunogen in the actual host that require protection can hasten vaccine development generate knowledge needed other similar vaccines. The African Swine Fever Virus (ASFV) causes a fatal hemorrhagic disease domestic swine and, at present, no treatment is available. Previous studies suggest induction ASFV-specific cytotoxic...
Abstract Subunit vaccine development against some complex pathogens require inclusion of multiple targets to optimize efficacy and or broaden protection diverse strains. To hasten development, it is rational evaluate a prototype in the actual outbred host that protection. African Swine Fever Virus (ASFV) high-consequence Transboundary Animal Disease pathogen for which there no treatment available. Current evidence shows ASFV can be stimulated with since pigs recover from infection less...