A5072088107- Parvovirus B19 Infection Studies
- Cytomegalovirus and herpesvirus research
- Polyomavirus and related diseases
- Virus-based gene therapy research
- Herpesvirus Infections and Treatments
- Antifungal resistance and susceptibility
- Vector-Borne Animal Diseases
- Animal Disease Management and Epidemiology
- Viral Infections and Immunology Research
- Antenna Design and Analysis
- Immune Cell Function and Interaction
- Renal Transplantation Outcomes and Treatments
- Prenatal Screening and Diagnostics
- Full-Duplex Wireless Communications
- Erythrocyte Function and Pathophysiology
- Transplantation: Methods and Outcomes
- Dermatological and COVID-19 studies
- SARS-CoV-2 and COVID-19 Research
- Fungal Infections and Studies
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Organ Donation and Transplantation
- Mosquito-borne diseases and control
- Plant Pathogens and Fungal Diseases
- Mycobacterium research and diagnosis
Eurofins (United States)
2018-2025
Eurofins (Denmark)
2024
Missouri College
2003-2007
University of Missouri
2001-2007
Plum Island Animal Disease Center
1998-1999
Agricultural Research Service
1995
National Animal Disease Center
1994
Donor-derived cell-free DNA (dd-cfDNA) has been evaluated as a rejection marker in organ transplantation. This study sought to assess the utility of dd-cfDNA diagnose graft injury liver transplant recipients (LTR) and predictive biomarker prior different causes dysfunction. Plasma from single multicenter LTR cohorts was analyzed for dd-cfDNA. Phenotypes treated biopsy-proven acute (AR, N = 57), normal function (TX, 94), dysfunction no (ADNR; 68) were divided into training test sets. In set,...
Parvovirus B19 (B19V) infects human erythroid progenitor cells (EPCs) and causes several hematological disorders fetal hydrops. Amino acid (aa) 5-68 of minor capsid protein VP1 (VP1u
Parvovirus B19 (B19V) infection is highly restricted to human erythroid progenitor cells. Although previous studies have led the theory that basis of this tropism receptor expression, has been questioned by more recent observation. In study reported here, we investigated tropism, and a potential role erythropoietin (Epo) signaling, in cells (EPCs) expanded ex vivo from CD34(+) hematopoietic absence Epo (CD36(+)/Epo(-) EPCs). We show, first, CD36(+)/Epo(-) EPCs do not support B19V...
Human parvovirus B19 (B19V) causes a variety of human diseases. Disease outcomes bone marrow failure in patients with high turnover red blood cells and immunocompromised conditions, fetal hydrops pregnant women are resulted from the targeting destruction specifically erythroid progenitors by B19V. Although ex vivo expanded progenitor recently used for studies B19V infection highly permissive, they produce progeny viruses inefficiently. In current study, we aimed to identify mechanism that...
Abstract Background BK polyomavirus (BKPyV) is associated with symptomatic hemorrhagic cystitis after hematopoietic cell transplantation (HCT). Little known about the host immune response, effectiveness of antiviral treatment, or impact asymptomatic replication on long-term kidney function. Methods In children and young adults undergoing allogeneic HCT, we quantified BKPyV viruria viremia (pre-HCT at Months 1–4, 8, 12, 24 post-HCT) tested associations peak ≥10 000 ≥109 copies/mL estimated...
ABSTRACT The pre-mRNA processing strategy of the B19 virus is unique among parvoviruses. virus-generated pre-mRNAs are transcribed from a single promoter and extensively processed by alternative splicing polyadenylation to generate 12 transcripts. Blockage production full-length transcripts at internal site [(pA)p] was previously reported be limiting step in permissiveness. We show here that absence genome replication, RNAs (pA)p favored cells which both permissive nonpermissive for viral...
Abstract Background Predicting respiratory viral infection (RVI) outcomes may lead to earlier intervention and improved prognosis in immunocompromised hosts. A microarray RNA-Seq results meta-analysis identified a conserved gene expression host response RVI of 396 genes called the Meta-Virus Signature (MVS). While MVS score positively correlates severity, 42-gene subset Severe-or-Mild (SoM) distinguishes severity groups with higher accuracy (PMID 33765435). This assay has not been validated...
Abstract Background Mucormycosis are diseases with high morbidity and mortality caused by fungal species belonging to the order Mucorales. The intended use of a Mucorales qPCR assay is as an adjunct microbiological, clinical, radiologic means diagnosing invasive mucormycosis (IM). Serum collection non-invasive alternative BAL biopsy. Our design process included sequences from all within genera: Cunninghamella, Lichtheimia, Absidia, Apophysomyces, Mucor, Rhizomucor, Rhizopus Saksenaea....
Abstract Background Fungal and acid-fast bacteria (AFB) infections can be a serious complication for immunosuppressed patients. Identification of the pathogen involved in invasive fungal/AFB (IFIs) allow reduced time to initiation appropriate targeted therapy through elimination ineffective therapies. Current diagnostics suffer from low sensitivity/specificity, invasiveness (e.g., biopsy or antigen), have long turnaround times culture), and/or require an priori hypothesis regarding causative...
Abstract Background Cytomegalovirus ( CMV ) infections are a major cause of disease among immunocompromised patients. Prolonged antiviral therapy is often necessary to prevent or treat disease, and may lead development resistance AVR ). Timely identification viral mutations conferring essential for effective patient management. Methods Amplification by polymerase chain reaction followed bi‐directional nucleotide sequencing was performed relevant regions the UL 97 54 genes. Results from 570...
ABSTRACT Although the Malawi Lil20/1 (MAL) strain of African swine fever virus (ASFV) was isolated from Ornithodoros sp. ticks, our attempts to experimentally infect ticks by feeding them this failed. Ten different collections Ornithodorus porcinus and one collection O. domesticus were orally exposed a high titer MAL. At 3 weeks postinoculation (p.i.), <25% contained detectable virus, with viral titers <4 log 10 50% hemadsorbing doses/ml. Viral declined undetectability in >90% 5...
African swine fever virus (ASFV) is the only known DNA arbovirus and sole member of family Asfarviridae. It causes a lethal, hemorrhagic disease in domestic pigs. ASFV enzootic sub-Saharan Africa maintained sylvatic cycle by infecting both wild members Suidae (e.g. warthogs) argasid tick Ornithodoros porcinus porcinus. The pathogenesis O. ticks characterized low infectious dose, lifelong infection, efficient transmission to pigs ticks, mortality until after first oviposition. warthogs an...
Equine recurrent uveitis (ERU) is the most frequent cause of blindness in horses worldwide. Leptospira has been implicated as an etiologic agent some cases ERU and detected fresh ocular tissues affected horses. The objective this study was to determine presence antigen DNA fixed equine with end-stage ERU. Sections eyes from 30 were obtained. Controls included 1) 10 normal 2) a nonrecurrent form uveitis. experimental group consisted diagnosed based on clinical signs histologic lesions....
During infection of human parvovirus B19 (B19V), one viral precursor mRNA (pre-mRNA) is transcribed by a single promoter and alternatively spliced polyadenylated. Here, we identified novel cis-acting sequence (5'-GUA AAG CUA CGG GAC GGU-3'), intronic splicing enhancer 3 (ISE3), which lies 72 nucleotides upstream the second splice acceptor (A2-2) site intron that defines exon encoding 11-kDa nonstructural protein. RNA binding motif protein 45 (RBM45) specifically binds to ISE3 with high...
An African swine fever virus (ASFV) ORF, 8CR, with similarity to the C-type lectin family of adhesion proteins has been described in pathogenic isolate Malawi Lil-20/1. The 8CR cellular and poxvirus genes associated cell adhesion, recognition infectivity suggested that may be significance ASFV-host interactions. Sequence analysis ORF from additional ASFV isolates demonstrated conservation among both pig tick sources. Northern blot mRNA transcription late replication cycle. A Lil-20/1...
Necropsy of an older dog submitted for evaluation renal and central nervous system disease revealed histologic lesions compatible with West Nile viral encephalitis myocarditis, as seen in other species. Using reverse transcriptase–polymerase chain reaction detection envelope sequences, RNA was detected most organs, quantitative polymerase that at least 1,000 times more present kidney than brain, heart, spleen, or lung. Immunohistochemical the intense staining antigens tubular epithelium...
Parvovirus B19 (B19V) infection causes diseases in humans ranging from the mild erythema infectiosum to severe hematological disorders. The unique region of minor structural protein VP1 (VP1u) 227 amino acids harbors strong neutralizing epitopes which elicit dominant immune responses patients. Recent studies have shown that VP1u selectively binds and enters B19V permissive cells through an unknown cellular proteinaceous receptor. In present study, we demonstrated purified recombinant...
To develop partial budgets of the economic costs 2 test strategies for screening cattle persistent infection with bovine viral diarrhea virus (BVDV).Partial budget analysis.938 calves arriving at stocker operations.Calves were tested to determine prevalence BVDV infection. Test that evaluated included a single-test strategy consisting immunohistochemical staining skin biopsy specimens from all animals and 2-test polymerase chain reaction (PCR) assaying pooled blood samples followed by in...
Lytic infection of human parvovirus B19 (B19V) takes place exclusively in erythroid progenitor cells bone marrow and fetal liver, which disrupts erythropoiesis. During infection, B19V expresses three nonstructural proteins (NS1, 11-kDa, 7.5-kDa) two structural (VP1 VP2). While NS1 is essential for DNA replication, 11-kDa enhances viral replication significantly. In this study, we confirmed the enhancement role elucidated underlying mechanism. We found that specially interacts with cellular...
Porcine reproductive and respiratory syndrome virus (PRRSV) continues to be a major economic problem for swine industries worldwide despite several disease-reduction strategies such as age-segregated early weaning all-in-all-out pig movement. Routine diagnosis of PRRSV is carried out by the combined use an antibody-detecting enzyme-linked immunosorbent assay (ELISA), immunofluorescence, reverse transcription-polymerase chain reaction, isolation. These assays require specialized laboratory...