A5060169767- RNA Research and Splicing
- Amyotrophic Lateral Sclerosis Research
- SARS-CoV-2 and COVID-19 Research
- Protease and Inhibitor Mechanisms
- SARS-CoV-2 detection and testing
- Coronary Interventions and Diagnostics
- Dupuytren's Contracture and Treatments
- Heat shock proteins research
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- S100 Proteins and Annexins
- Natural Antidiabetic Agents Studies
- Lymphatic System and Diseases
- Dermatological and Skeletal Disorders
- Liver Disease and Transplantation
- Advanced biosensing and bioanalysis techniques
- Colorectal Cancer Treatments and Studies
- Advanced Neuroimaging Techniques and Applications
- Angiogenesis and VEGF in Cancer
- Psidium guajava Extracts and Applications
- Redox biology and oxidative stress
- Vascular anomalies and interventions
- Inflammatory Bowel Disease
- Kruppel-like factors research
- bioluminescence and chemiluminescence research
Hokkaido University
2021-2025
Advanced Life Science Institute
2025
Toho University
2009-2024
Toho University Omori Medical Center
2009-2024
Prefectural University of Hiroshima
2011
University of California, Irvine
2008-2010
Keio University
2010
University of California System
2009
University of California, Los Angeles
2006
Tsukuba Memorial Hospital
2004
Although the SARS-CoV-2 epidemic worldwide has gradually decreased, in some areas, situation not yet been stamped and become a global health emergency. It is quite possible that we could again be threatened by new coronavirus. Therefore, nucleotide analog drugs vaccines or using drug repositioning for still developed, their safety efficacy against COVID‐19 remains underexplored. Malabaricone C 2,6-dihydroxyphenyl acylphenol found edible plants such as mace spice of nutmeg derived from seeds...
Abstract Carboxy terminal fragments (CTFs) of TDP-43 contain an intrinsically disordered region (IDR) and form cytoplasmic condensates containing amyloid fibrils. Such are toxic associated with pathogenicity in amyotrophic lateral sclerosis. However, the molecular details how domain CTFs leads to condensation cytotoxicity remain elusive. Here, we show that truncated RNA/DNA-recognition motif (RRM) at N-terminus structural transition IDR, whereas IDR itself is difficult assemble even if they...
Macrophage apoptosis and MMP activity contribute to vulnerability of atherosclerotic plaques rupture. By employing molecular imaging techniques, we investigated if release are interlinked. Atherosclerosis was produced in rabbits receiving high-cholesterol diet (HC), who underwent dual radionuclide with 99mTc-labeled matrix metalloproteinase inhibitor (MPI) 111In-labeled annexin A5 (AA5) using micro-SPECT/CT. %ID/g MPI AA5 uptake measured, followed by histological characterization....
Arsenite [As(III)] is a toxic substance widely present on Earth, and the development of low-cost simple microbial-based As(III) sensors has been attracting attention. Recently, we discovered that protein LuxR, which contains multiple cysteine residues with high affinity for As(III), forms an insoluble structure upon binding to exhibits OFF-switching properties as quorum sensing transcriptional activator. Based this property, LuxR sensor operates new principle distinct from conventional...
Abstract Excess administration of glutamate is known to induce Ca 2+ overload in neurons, which the first step excitotoxicity. Although some reports have suggested a role for Mg excitotoxicity, little about its actual contribution. To investigate we simultaneously measured intracellular and , using fluorescent dyes, Fura red, probe, KMG‐104, highly selective probe developed by our group, respectively. Administration 100 μM supplemented with 10 glycine rat hippocampal neurons induced an...
Abstract Background and Aims: Cancer invasion metastasis are critical events for patient prognosis; however, the most important step in whole process of lymph node (LN) gastric cancer remains obscure. In this study, significance cell behaviors, such as detachment, stromal lymphatic on regional LN was investigated by comprehensive immunohistochemistry. Methods: A total 210 cases with were selected. These consisted 105 (LN[+] group) without (LN[–] group). Both groups exhibited same depth...
TAR DNA/RNA-binding protein 43 kDa (TDP-43) proteinopathy is a hallmark of neurodegenerative disorders, such as amyotrophic lateral sclerosis, in which cytoplasmic aggregates containing TDP-43 and its C-terminal fragments, TDP-25, are observed degenerative neuronal cells. However, few reports have focused on small molecules that can reduce their aggregation cytotoxicity. Here, we show short RNA repeats GGGGCC AAAAUU suppressors TDP-25. TDP-25 interacts with these RNAs, well TDP-43, despite...
Stress granules (SGs) are cytoplasmic condensates composed of various proteins and RNAs that protect translation-associated machinery from harmful conditions during stress. However, the method spatiotemporal inactivation such as SGs in live cells to study cellular phenotypes is still process being demonstrated. Here, we show by chromophore-associated light (CALI) using a genetically encoded red fluorescence protein (SuperNova-Red) photosensitizer leads differences cell viability recovery...
Introduction Filgotinib is a JAK-1 selective inhibitor approved for ulcerative colitis (UC) treatment in Japan. Its effectiveness has been confirmed but remains unknown actual clinical practice. Therefore, we aimed to evaluate the and safety of filgotinib identify suitable patients Japanese population. Methods We retrospectively reviewed background, course, laboratory data treated with 200 mg UC between May 2022 December 2023. Results The median observation period 25 was 232 days...
Abstract Carboxy terminal fragments (CTFs) of TDP-43 contain an intrinsically disordered region (IDR) and form cytoplasmic condensates containing amyloid fibrils. Such are toxic associated with pathogenicity in amyotrophic lateral sclerosis. However, the molecular details how domain CTFs leads to condensation cytotoxicity remain elusive. Here, we show that truncated RNA/DNA-recognition motif (RRM) at N-terminus structural transition IDR, whereas IDR itself is difficult assemble even if they...
Abstract TAR DNA/RNA-binding protein 43 kDa (TDP-43) proteinopathy is a hallmark of neurodegenerative disorders such as amyotrophic lateral sclerosis, in which cytoplasmic aggregates containing TDP-43 and its C-terminal fragments, TDP25, are observed degenerative neuronal cells. However, few reports have focused on small molecules that can reduce their aggregation cytotoxicity. Here, we show short RNA repeats GGGGCC AAAAUU aggregation-suppressors TDP25. TDP25 interacts with these RNAs, well...
Abstract Infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the cause of coronavirus disease 2019 (COVID-19), is initiated by interaction between a receptor protein, angiotensin-converting enzyme type 2 (ACE2) on cell surface, and viral spike (S) protein. This similar to mechanism in SARS-CoV, close relative SARS-CoV-2, which was identified 2003. Drugs antibodies that inhibit ACE2 S proteins could be key therapeutic methods for preventing infection replication...
Abstract The omicron variant of SARS-CoV-2 is responsible for the COVID-19 pandemic, serving as a significant origin variants still being detected today. It affects spike protein that most vaccines used to target when Omicron strain was discovered. Here, we demonstrate receptor binding domain (RBD) exhibits an increased affinity human angiotensin-converting enzyme type 2 (hACE2) viral cell compared prototype RBD. We also identified β- and γ-actin are Omicron-specific partners Protein complex...
The omicron variant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was identified in 2021 as a with heavy amino acid mutations the spike protein, which is targeted by most vaccines, compared to previous variants. Amino substitutions proteins may alter their affinity for host viral receptors and interactome. Here, we found that receptor-binding domain (RBD) SARS-CoV-2 exhibited an increased human angiotensin-converting enzyme 2, cell receptor, prototype RBD. Moreover, β-...
Infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the cause of coronavirus disease 2019 (COVID-19), is initiated by interaction between a receptor protein, angiotensin-converting enzyme type 2 (ACE2) on cell surface, and viral spike (S) protein. This similar to mechanism in SARS-CoV, close relative SARS-CoV-2, which was identified 2003. Drugs antibodies that inhibit ACE2 S proteins could be key therapeutic methods for preventing infection replication COVID-19....
Protein aggregation is a hallmark of neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS), Alzheimer's disease (AD), Parkinson's (PD), Huntington's (HD), and so on. To detect analyze soluble or diffuse protein oligomers aggregates, fluorescence correlation spectroscopy (FCS), which can the diffusion speed brightness single particle with molecule sensitivity, has been used. However, proper procedure know-how for detection have not widely shared. Here, we show standard FCS...
Abstract Stress granules (SGs) are cytoplasmic condensates composed of various proteins and RNAs that protect translation-associated machinery from harmful conditions during stress. However, the method spatio-temporal inactivation such as SGs in live cells to study cellular phenotypes is still process being demonstrated. Here, we show SG by chromophore-associated light (CALI) using a genetically encoded red fluorescence protein (SuperNova-Red) photosensitizer leads differences cell viability...