Abstract Near-infrared photoimmunotherapy (NIR-PIT) is a newly developed and selective cancer treatment that induces necrotic immunogenic cell death utilizes mAb conjugated to photo-absorber dye, IR700DX, activated by NIR light. Although CD44 surface marker associated with drug resistance, anti-CD44-IR700 NIR-PIT results in inhibited growth prolonged survival multiple tumor types. Meanwhile, CD25-targeted has been reported achieve local depletion of FOXP3+CD25+CD4+ regulatory T cells (Treg),...

Programmed cell death 1 (PD-1) blockade therapy can result in dramatic responses some patients with cancer. However, about 15% of receiving PD-1 experience rapid tumor progression, a phenomenon termed "hyperprogressive disease" (HPD). The mechanism(s) underlying HPD has been difficult to uncover because is challenging reproduce animal models. Near-infrared photoimmunotherapy (NIR-PIT) method by which specific cells the microenvironment (TME) be selectively depleted without disturbing other...

Regulatory T (Treg) cells play a major role in immune suppression permitting tumors to evade surveillance. Depletion of intratumoral Treg can result tumor regression. However, systemic depletion Tregs may also induce autoimmune adverse events. Near-infrared photoimmunotherapy (NIR-PIT) is newly developed cell-specific cancer therapy that locally kills specific the tumor. Antibody-photoabsorber (IRDye700DX) conjugates (APC) are injected and bind tumor, subsequent administration NIR light...

Near-infrared photoimmunotherapy (NIR-PIT) is a cancer treatment that utilizes antibody-photoabsorber (IR700) conjugates to selectively kill target cells by exposing them NIR light. Cytotoxic T-lymphocyte antigen 4 (CTLA4) major immune checkpoint ligand mediating antitumor suppression. Local depletion of CTLA4 expressing in the tumor bed with NIR-PIT could enhance responses both blocking CTLA4-axis and depleting suppressive cells. The aim this study evaluate efficacy CTLA4-targeted using...

Near-infrared photoimmunotherapy (NIR-PIT) is a new type of cancer therapy that employs antibody-IRDye700DX conjugates (AbPCs) and near-infrared (NIR) light at wavelength 689 nm, the excitation IR700. Administered intravenously, injected AbPCs bind specifically to cells expressing target antigen, whereupon NIR exposure causes rapid, selective killing. This process induces an anticancer T cell response, leading sustained host immune response. Programmed death ligand-1 (PD-L1) major inhibitory...

Near-infrared photoimmunotherapy (NIR-PIT) is a cell-selective cancer therapy employing monoclonal antibody-photoabsorber conjugates (APCs) and near-infrared (NIR) light. When exposed to NIR light, the photoabsorber, IR700, releases an axial ligand, resulting in transition of remaining molecule from water-soluble hydrophobic. This results death APC-bound cells by physical damage cell membrane. The amount released IR700 ligand reflects completeness photochemical reaction and, therefore, can...

Near-infrared photoimmunotherapy (NIR-PIT) is a highly selective cancer treatment that employs an antibody photoabsorber conjugate (APC) composed of targeting monoclonal (mAb) conjugated with photoactivatable phthalocyanine-derivative dye. Once injected and allowed to bind tumor, the APC activated by local near-infrared light which kills cells induces strong immune response in tumor microenvironment unmasking new antigens emerging from damaged cells. Due its ability incite reaction, even...

Near infrared photoimmunotherapy (NIR-PIT) is a newly developed and highly selective cancer treatment that induces necrotic/immunogenic cell death. It employs monoclonal antibody (mAb) conjugated to photo-absorber dye, IRDye700DX, which activated by NIR light. Tumor-targeting NIR-PIT also at least partly mediated profound immune response against the tumor. Cytotoxic T-lymphocyte antigen-4 (CTLA4) widely recognized as major checkpoint protein, inhibits tumors therefore, target for systemic...

near-infrared photoimmunotherapy (NIR-PIT) is a cancer treatment that uses antibody-photoabsorber (IRDye700DX, IR700) conjugates (APCs) which bind to target cells and are photoactivated by NIR light inducing rapid necrotic cell death. NIR-PIT targeting human epidermal growth factor receptor (hEGFR) has been shown destroy hEGFR expressing tumor be effective in immunodeficient mouse models. can also targeted the microenvironment, for instance, CD25-targeted used selectively deplete regulatory...

Near-infrared photoimmunotherapy (NIR-PIT) is a newly developed cancer treatment that utilizes an antibody-photoabsorber-conjugate (AbPC) combined with NIR light. The AbPC injected and binds to the tumor whereupon light irradiation causes photochemical reaction selectively kills cells. NIR-PIT ideal for surface-located skin cancers such as melanoma. However, there concern pigment in melanoma lesions could interfere delivery, rendering ineffective. We investigated efficacy of CD29-...

Abstract Temporal–spatial variations in Late Cenozoic volcanic activity the Chugoku area, southwest Japan, have been examined based on 108 newly obtained K–Ar ages. Lava samples were collected from eight Quaternary provinces (Daisen, Hiruzen, Yokota, Daikonjima, Sambe, Ooe–Takayama, Abu and Oki) a Tertiary cluster (Kibi Province) to cover almost all geological units province. Including published age data, total of 442 radiometric ages are now available. Across‐arc an area approximately 500...

Near infrared (NIR) fluorescent probes are attractive tools for biomedical in vivo imaging due to the relatively deeper tissue penetration and lower background autofluorescence. Activatable turned on only after binding their target, further improving target ratios. However, number of available activatable NIR is limited. In this study, we introduce two types fluorophores derived from bacteriochlorin: chlorin–bacteriochlorin energy-transfer dyads boron-dipyrromethene (BODIPY)-bacteriochlorin...

Near infrared photoimmunotherapy (NIR-PIT) is a newly developed and highly selective cancer treatment that employs monoclonal antibody (mAb) conjugated to photo-absorber dye, IRDye700DX, which activated by 690 nm light. Cancer cell-targeted NIR-PIT induces rapid necrotic/immunogenic cell death (ICD) antitumor host immunity including re-priming proliferation of T cells. Interleukin-15 (IL-15) cytokine activates natural killer (NK)-, B- T-cells while having minimal effect on regulatory cells...

Abstract Regulatory T cells (Tregs) are known to inhibit antitumor immunity, yet the specific mechanism by which intratumoral Tregs promote tumor growth remains unclear. To better understand roles of Tregs, we selectively depleted tumor-infiltrating using anti-CD25-F(ab′)2 near-infrared photoimmunotherapy. Depletion induced transient but synchronized IFNγ expression in CD8 and natural killer (NK) cells. Despite small fraction NK contained within examined tumors, produced these led efficient...

Abstract Near-infrared photoimmunotherapy (NIR-PIT) is a new cancer treatment that utilizes antibody–IRDye700DX (IR700) conjugates. The clinical use of NIR-PIT has recently been approved in Japan for patients with inoperable head and neck targeting human epidermal growth factor receptor (hEGFR). Previously, cytotoxic T-lymphocyte antigen 4 (CTLA4)–targeted shown to strongly inhibit tumor progression prolonged survival was seen different models due enhanced T-cell–mediated antitumor immunity....

The immune system is recognized as an important factor in regulating the development, progression, and metastasis of cancer. Myeloid-derived suppressor cells (MDSCs) are a major immune-suppressive cell type by interfering with T activation, promoting effector apoptosis, inducing regulatory expansion. Consequently, reducing or eliminating MDSCs has become goal some systemic immunotherapies. However, systemically MDSCs, unwanted side effects can occur. Near-infrared photoimmunotherapy...

Triple-negative breast cancer (TNBC) is the most aggressive subtype of cancer, and conventional chemotherapy molecular-targeted therapies show limited efficacy. Near-infrared photoimmunotherapy (NIR-PIT) a new anticancer treatment that selectively damages cell membrane cells based on NIR light-induced photochemical reactions antibody (Ab)-photoabsorber (IRDye700Dx) conjugate membrane. TNBC known to express several adhesion molecules surface providing potential target for therapy. Here, we...

Abstract Near-infrared photoimmunotherapy (NIR-PIT) is a new cancer treatment that uses an antibody-IRDye700DX (IR700) conjugate binds to target followed by the application of NIR light results in dramatic changes solubility leading rapid cell membrane damage and highly immunogenic death. NIR-PIT has been used clinically treating advanced head neck cancers using anti-EGFR antibody-IR700 conditionally approved for clinical use Japan. can be employed wide range targeting antibodies. Podoplanin...

The bones are a common site for metastasis arising from solid tumors such as breast and prostate cancer. Chemotherapy, including immunotherapy, is rarely curative. Radiotherapy with pain palliation can temporize bone metastases but generally considered short-term solution retreatment difficult. Surgery often necessary, yet recovery times might exceed life expectancy. Therefore, there need to develop new approaches that effective minimally invasive. Near-infrared photoimmunotherapy (NIR-PIT)...

Near-infrared photoimmunotherapy (NIR-PIT) employs molecularly targeted antibodies conjugated with a photoabsorbing silicon-phthalocyanine dye derivative which binds to cancer cells. Application of NIR light following binding the antibody-photoabsorber conjugates (APCs) results in ligand release on dye, dramatic changes solubility APC-antigen complex, and rapid, irreversible cell membrane damage cells highly selective manner, resulting immunogenic death. Clinically, this process edema after...

Near-infrared photoimmunotherapy (NIR-PIT) is a newly developed cancer treatment that uses antibody-IRDye700DX (IR700) conjugates and was recently approved in Japan for patients with inoperable head neck cancer. Exposure of the tumor NIR light at wavelength 690 nm leads to physicochemical changes antibody-IR700 conjugate-cell receptor complex, resulting increased hydrophobicity damage integrity cell membrane. However, it important be completely exposed during NIR-PIT, thus, method provide...

Near-infrared photoimmunotherapy (NIR-PIT) is a cell selective cancer therapy that uses an antibody-photoabsorber (IRDye700DX, IR700) conjugate (APC) and NIR light. NIR-PIT targeting epidermal growth factor receptor (EGFR) in head neck (HNC) was conditionally approved Japan 2020. APC-bound tumors can be detected using endoscopic fluorescence imaging, whereas light delivered fiber optics. The aims of this study were: (1) to assess the feasibility orthotopic HNC model CD44-expressing MOC2-luc...