A5082791600- Microbial Community Ecology and Physiology
- Wastewater Treatment and Nitrogen Removal
- Fecal contamination and water quality
- Urban and spatial planning
- Water Treatment and Disinfection
- Protist diversity and phylogeny
- Microbial Fuel Cells and Bioremediation
- Vibrio bacteria research studies
- Soil Carbon and Nitrogen Dynamics
- Genomics and Phylogenetic Studies
- Gut microbiota and health
- Viral gastroenteritis research and epidemiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Communication in Education and Healthcare
- Salmonella and Campylobacter epidemiology
- Constructed Wetlands for Wastewater Treatment
- Bacteriophages and microbial interactions
- EFL/ESL Teaching and Learning
- Escherichia coli research studies
- Antibiotic Resistance in Bacteria
- Metabolism and Genetic Disorders
- Peroxisome Proliferator-Activated Receptors
- Biosensors and Analytical Detection
- Polar Research and Ecology
- Ammonia Synthesis and Nitrogen Reduction
University of Minnesota
2016-2025
Biotechnology Institute
2015-2024
University of Minnesota System
2015-2024
Tohoku Institute of Technology
2006-2024
Bioversity International
2023
International Gorilla Conservation Programme
2023
Hokkaido University
2011-2020
Nagoya University
2020
Kindai University
2009-2018
Nagoya Institute of Technology
2018
Abstract While soil erosion drives land degradation, the impact of on microbial communities and multiple functions remains unclear. This hinders our ability to assess true ecosystem services restore eroded environments. Here we examined effect at two sites with contrasting texture climates. Eroded plots had lower network complexity, fewer taxa, associations among relative non-eroded plots. Soil also shifted community composition, decreased abundances dominant phyla such as Proteobacteria,...
ABSTRACT The presence of Escherichia coli in water is used as an indicator fecal contamination, but recent reports indicate that soil populations can also be detected tropical, subtropical, and some temperate environments. In this study, we report viable E. were repeatedly isolated from northern soils three Lake Superior watersheds October 2003 to 2004. Seasonal variation the population density soilborne was observed; greatest cell densities, up 3 × 10 CFU/g soil, found summer fall (June...
Escherichia coli is naturally present in the intestinal tracts of warm-blooded animals. Since E. released into environment through deposition fecal material, this bacterium widely used as an indicator contamination waterways. Recently, research efforts have been directed towards identification potential sources impacting waterways and beaches. This often referred to microbial source tracking. However, recent studies reported that can become "naturalized" soil, sand, sediments, algae...
Afforestation is an effective approach to rehabilitate degraded ecosystems, but often depletes deep soil moisture. Presently, it not known how afforestation-induced decrease in moisture affects microbial community and functionality, hindering our ability understand the sustainability of rehabilitated ecosystems. To address this issue, we examined impacts 20 years afforestation on bacterial community, co-occurrence pattern, functionalities along vertical profile (0-500 cm depth) a semiarid...
The Duluth Boat Club (DBC) Beach, located in the Duluth−Superior harbor of Lake Superior, is frequently closed summer due to high counts Escherichia coli, an indicator fecal contamination. However, sources bacteria contributing beach closure are currently unknown. In this study, we investigated potential E. coli contaminating DBC by using modified rep-PCR (HFERP) DNA fingerprinting. Over 3600 strains were obtained from 55 lake water, 25 sediment, and 135 sand samples taken five transects at...
The common occurrence of Escherichia coli in temperate soils has previously been reported, however, there are few studies to date characterize its source, distribution, persistent capability and genetic diversity. In this study, undisturbed, forest within six randomly selected 0.5 m2 exclosure plots (covered by netting 2.3 mm2 mesh size) were monitored from March October 2003 for E. order describe numerical population characteristics. Culturable occurred 88% the samples collected, with...
We examined nitrate-dependent Fe(2+) oxidation mediated by anaerobic ammonium (anammox) bacteria. Enrichment cultures of "Candidatus Brocadia sinica" anaerobically oxidized and reduced NO3(-) to nitrogen gas at rates 3.7 ± 0.2 1.3 0.1 (mean standard deviation [SD]) nmol mg protein(-1) min(-1), respectively (37°C pH 7.3). This nitrate reduction rate is an order magnitude lower than the anammox activity "Ca. (10 75 NH4(+) min(-1)). A (15)N tracer experiment demonstrated that coupling reaction...
ABSTRACT Cladophora glomerata , a macrophytic green alga, is commonly found in the Great Lakes, and significant accumulations occur along shorelines during summer months. Recently, has been shown to harbor high densities of fecal indicator bacteria Escherichia coli enterococci. may also human pathogens; however, until now, no studies address this question have performed. In present study, we determined whether attached obtained from Lake Michigan Burns Ditch (Little Calumet River, Indiana)...
ABSTRACT The direct quantification of multiple pathogens has been desired for diagnostic and public health purposes a long time. In this study, we applied microfluidic quantitative PCR (qPCR) technology to the simultaneous detection food- waterborne pathogens. system, singleplex qPCR assays were run under identical conditions in nanoliter-volume chambers that are present high densities on chip. First, developed 18 TaqMan could be same by using prevalidated probes. Specific sensitive was...
A large number of repetitive DNA sequences are found in multiple sites the genomes numerous bacteria, archaea and eukarya. While functions many these sequence elements unknown, they have proven to be useful as basis several powerful tools for use molecular diagnostics, medical microbiology, epidemiological analyses environmental microbiology. The sequence-based PCR or rep-PCR fingerprint technique uses primers targeting generate unique profiles 'fingerprints' individual microbial strains....
To secure food and water safety, quantitative information on multiple pathogens is important. In this study, we developed a microfluidic PCR (MFQPCR) system to simultaneously quantify 11 major human viral pathogens, including adenovirus, Aichi virus, astrovirus, enterovirus, norovirus, rotavirus, sapovirus, hepatitis A E viruses. Murine norovirus mengovirus were also quantified in our MFQPCR as sample processing control an internal amplification control, respectively. River contaminated with...
Abstract Growing concerns about the global antimicrobial resistance crisis require a better understanding of how antibiotic persists in soil and exposure impacts microbial communities. In agroecosystems, these responses are complex because environmental factors may influence communities respond to manure exposure. The study aimed determine type moisture alter additions from cattle treated with antibiotics. Soil microcosms were constructed using two types at 15, 30, or 45% moisture....
Escherichia coli strains in water may originate from various sources, including humans, farm and wild animals, waterfowl, pets. However, potential human health hazards associated with E. present animal hosts are not well known. In this study, diverse sources Minnesota western Wisconsin were analyzed for the presence of genes coding virulence factors by using multiplex PCR biochemical reactions. Of 1,531 isolates examined, 31 (2%) found to be Shiga toxin-producing (STEC) strains. The majority...
Epilithic periphyton communities were sampled at three sites on the Minnesota shoreline of Lake Superior from June 2004 to August 2005 determine if fecal coliforms and Escherichia coli present throughout ice-free season. Fecal coliform densities increased up 4 orders magnitude in early summer, reached peaks 1.4x10(5) CFU cm-2 by late July, decreased during autumn. Horizontal, fluorophore-enhanced repetitive-PCR DNA fingerprint analyses indicated that source for 2% 44% E. bacteria isolated...