A5074644944- Wastewater Treatment and Nitrogen Removal
- Microbial Community Ecology and Physiology
- Microbial Fuel Cells and Bioremediation
- Water Treatment and Disinfection
- Cardiac Structural Anomalies and Repair
- Cardiac Valve Diseases and Treatments
- Aortic Disease and Treatment Approaches
- Advanced biosensing and bioanalysis techniques
- Mechanical Circulatory Support Devices
- Neuroscience and Neuropharmacology Research
- Odor and Emission Control Technologies
- Membrane Separation Technologies
- Cardiac and Coronary Surgery Techniques
- Neurotransmitter Receptor Influence on Behavior
- Molecular Sensors and Ion Detection
- Peripheral Artery Disease Management
- Aortic aneurysm repair treatments
- Tracheal and airway disorders
- Epilepsy research and treatment
- Anaerobic Digestion and Biogas Production
- Constructed Wetlands for Wastewater Treatment
- Pharmacological Receptor Mechanisms and Effects
- Pharmaceutical and Antibiotic Environmental Impacts
- Vascular Procedures and Complications
- Mine drainage and remediation techniques
Hokkaido University
2016-2025
Higashi Takarazuka Satoh Hospital
2011-2024
Faculty (United Kingdom)
2020
Engineering (Italy)
2020
Sapporo Science Center
2020
Koga Hospital
2007-2016
Hirosaki University
2004-2008
Fukuoka Tokushukai Hospital
2008
Hachinohe Institute of Technology
2002-2006
Hiroshima University
2006
ABSTRACT We investigated the in situ spatial organization of ammonia-oxidizing and nitrite-oxidizing bacteria domestic wastewater biofilms autotrophic nitrifying by using microsensors fluorescent hybridization (FISH) performed with 16S rRNA-targeted oligonucleotide probes. The combination these techniques made it possible to relate microbial activity directly occurrence bacterial populations. In revealed that belonging genus Nitrosomonas were numerically dominant both types biofilms....
Microbially induced concrete corrosion (MICC) in sewer systems has been a serious problem for long time. A better understanding of the succession microbial community members responsible production sulfuric acid is essential efficient control MICC. In this study, sulfur-oxidizing bacteria (SOB) bacterial on corroding system situ was investigated over 1 year by culture-independent 16S rRNA gene-based molecular techniques. Results revealed that at least six phylotypes SOB species were involved...
The present study investigated the phylogenetic affiliation and physiological characteristics of bacteria responsible for anaerobic ammonium oxidization (anammox); these were enriched in an anammox reactor with a nitrogen removal rate 26.0 kg N m −3 day −1 . identified as representing ‘ Candidatus Brocadia sinica’ on basis analysis rRNA operon sequences. Physiological examined growth rate, kinetics oxidation nitrite reduction, temperature, pH inhibition anammox. maximum specific (μ max ) was...
Summary To date, six candidate genera of anaerobic ammonium‐oxidizing (anammox) bacteria have been identified, and numerous studies conducted to understand their ecophysiology. In this study, we examined the physiological characteristics an anammox bacterium in genus ‘ C andidatus J ettenia’. Planctomycete KSU ‐1 was found be a mesophilic (20–42.5°C) neutrophilic ( pH 6.5–8.5) with maximum growth rate 0.0020 h −1 . cells showed typical structural features bacteria; i.e. 29 N 2 gas production...
We examined nitrate-dependent Fe(2+) oxidation mediated by anaerobic ammonium (anammox) bacteria. Enrichment cultures of "Candidatus Brocadia sinica" anaerobically oxidized and reduced NO3(-) to nitrogen gas at rates 3.7 ± 0.2 1.3 0.1 (mean standard deviation [SD]) nmol mg protein(-1) min(-1), respectively (37°C pH 7.3). This nitrate reduction rate is an order magnitude lower than the anammox activity "Ca. (10 75 NH4(+) min(-1)). A (15)N tracer experiment demonstrated that coupling reaction...
ABSTRACT We investigated autotrophic anaerobic ammonium-oxidizing (anammox) biofilms for their spatial organization, community composition, and in situ activities by using molecular biological techniques combined with microelectrodes. Results of phylogenetic analysis fluorescence hybridization (FISH) revealed that “ Brocadia ”-like anammox bacteria hybridized the Amx820 probe dominated, 60 to 92% total upper part (<1,000 μm) biofilm, where high activity was mainly detected The relative...
Although metabolic pathways and associated enzymes of anaerobic ammonium oxidation (anammox) 'Ca. Kuenenia stuttgartiensis' have been studied, those other anammox bacteria are still poorly understood. NO2- reduction to NO is considered be the first step in metabolism K. stuttgartiensis', however, Brocadia' lacks genes that encode canonical NO-forming nitrite reductases (NirS or NirK) its genome, which different from stuttgartiensis'. Here, we studied Brocadia sinica'. (15) N-tracer...
To verify whether members of the phylum
The vertical distribution of sulfate-reducing bacteria (SRB) in aerobic wastewater biofilms grown on rotating disk reactors was investigated by fluorescent situ hybridization (FISH) with 16S rRNA-targeted oligonucleotide probes. To correlate the SRB populations their activity, microprofiles O(2), H(2)S, NO(2)(-), NO(3)(-), NH(4)(+), and pH were measured microelectrodes. In addition, a cross-evaluation FISH microelectrode analyses performed comparing them culture-based approaches...
ABSTRACT The microbial community structure and spatial distribution of microorganisms their in situ activities anaerobic granules were investigated by 16S rRNA gene-based molecular techniques microsensors for CH 4 , H 2 pH, the oxidation-reduction potential (ORP). gene-cloning analysis revealed that clones related to phyla Alphaproteobacteria (detection frequency, 51%), Firmicutes (20%), Chloroflexi (9%), Betaproteobacteria (8%) dominated bacterial clone library, predominant archaeal library...
Effects of sulpiride on the central nervous system were studied in catalepsy induction (I) and antagonism to gnawing behaviour (II) induced by apomorphine methamphetamine normal rats, rotational (III) rats with substantia nigra unilaterally lesioned chronically microinjection 6-hydroxydopamine. Sulpiride was administered orally intraventricularly, effects compared those haloperidol chlorpromazine through same routes. In oral administration, almost inactive (I), several hundreds a thousand...
A draft genome sequence of an anaerobic ammonium-oxidizing (anammox) bacterium, "Candidatus Brocadia sinica," was determined by pyrosequencing and screening a fosmid library. 4.07-Mb comprising 3 contigs assembled, in which 3,912 gene-coding regions, 47 tRNAs, single rrn operon were annotated.
ABSTRACT A combination of fluorescence in situ hybridization, microprofiles, denaturing gradient gel electrophoresis PCR-amplified 16S ribosomal DNA fragments, and rRNA gene cloning analysis was applied to investigate successional development sulfate-reducing bacteria (SRB) community structure sulfide production activity within a biofilm growing under microaerophilic conditions (dissolved oxygen concentration the bulk liquid range 0 100 μM) presence nitrate. Microelectrode measurements...