ABSTRACT Ecophysiological interactions between the community members (i.e., nitrifiers and heterotrophic bacteria) in a carbon-limited autotrophic nitrifying biofilm fed only NH 4 + as an energy source were investigated by using full-cycle 16S rRNA approach followed microautoradiography (MAR)-fluorescence situ hybridization (FISH). Phylogenetic differentiation (identification) of bacteria was performed gene sequence analysis, FISH probes designed to determine structure spatial organization...

Microbially induced concrete corrosion (MICC) in sewer systems has been a serious problem for long time. A better understanding of the succession microbial community members responsible production sulfuric acid is essential efficient control MICC. In this study, sulfur-oxidizing bacteria (SOB) bacterial on corroding system situ was investigated over 1 year by culture-independent 16S rRNA gene-based molecular techniques. Results revealed that at least six phylotypes SOB species were involved...

The vertical distribution of sulfate-reducing bacteria (SRB) in aerobic wastewater biofilms grown on rotating disk reactors was investigated by fluorescent situ hybridization (FISH) with 16S rRNA-targeted oligonucleotide probes. To correlate the SRB populations their activity, microprofiles O(2), H(2)S, NO(2)(-), NO(3)(-), NH(4)(+), and pH were measured microelectrodes. In addition, a cross-evaluation FISH microelectrode analyses performed comparing them culture-based approaches...

The cross-feeding of microbial products derived from 14C-labeled nitrifying bacteria to heterotrophic coexisting in an autotrophic biofilm was quantitatively analyzed by using microautoradiography combined with fluorescence situ hybridization (MAR-FISH). After only were labeled [14C]bicarbonate, samples incubated and without NH4+ as a sole energy source for 10 days. transfer 14C originally incorporated into bacterial cells monitored time MAR-FISH. MAR-FISH analysis revealed that most...

ABSTRACT We simultaneously determined the phylogenetic identification and substrate uptake patterns of sulfate-reducing bacteria (SRB) inhabiting a sewer biofilm with oxygen, nitrate, or sulfate as an electron acceptor by combining microautoradiography fluorescent in situ hybridization (MAR-FISH) family- genus-specific 16S rRNA probes. The MAR-FISH analysis revealed that Desulfobulbus hybridized probe 660 was dominant SRB subgroup this biofilm, accounting for 23% total SRB. Approximately 9...

ABSTRACT A combination of fluorescence in situ hybridization, microprofiles, denaturing gradient gel electrophoresis PCR-amplified 16S ribosomal DNA fragments, and rRNA gene cloning analysis was applied to investigate successional development sulfate-reducing bacteria (SRB) community structure sulfide production activity within a biofilm growing under microaerophilic conditions (dissolved oxygen concentration the bulk liquid range 0 100 μM) presence nitrate. Microelectrode measurements...

Abstract Population dynamics of ammonia‐oxidizing bacteria (AOB) and uncultured Nitrospira ‐like nitrite‐oxidizing (NOB) dominated in autotrophic nitrifying biofilms were determined by using real‐time quantitative polymerase chain reaction (RTQ‐PCR) fluorescence situ hybridization (FISH). Although two techniques gave the comparable results, RTQ‐PCR assay was easier faster than FISH technique for quantification both dense microcolony‐forming biofilms. Using this assay, we could successfully...

ABSTRACT The succession of sulfur-oxidizing bacterial (SOB) community structure and the complex internal sulfur cycle occurring in wastewater biofilms growing under microaerophilic conditions was analyzed by using a polyphasic approach that employed 16S rRNA gene-cloning analysis combined with fluorescence situ hybridization, microelectrode measurements, standard batch reactor experiments. A complete established via S 0 accumulation within 80 days replicate. This development generally split...

A novel mesophilic, chemolithoautotrophic, sulfur-oxidizing bacterium, designated strain SO07(T), was isolated from a microaerobic waste-water biofilm. Chemolithoautotrophic growth observed with elemental sulfur, sulfide and thiosulfate as sole electron donors oxygen acceptor. Anaerobic heterotrophic were not observed. Nitrate used terminal The optimum pH temperature for 7.5 30 degrees C, respectively. major isoprenoid quinone Q-8. DNA G + C content of SO07(T) 47.1 mol%. Phylogenetic...

Abstract A fine‐scale in situ spatial organization of ammonia‐oxidizing bacteria (AOB) biofilms was investigated by combining molecular techniques (i.e., fluorescence hybridization (FISH) and 16S rDNA‐cloning analysis) microelectrode measurements. Important parameters AOB microcolonies such as size distribution areal cell density the were determined correlated with substrate microprofiles biofilms. In a nested rRNA‐targeted oligonucleotide probe set revealed two different populations AOB,...

We obtained dye-decolorizing bacteria from the hands of ordinary people with an efficiency approximately 40%. The were classified into azo group and anthraquinone group. former capable decolorizing real textile wastewater, whereas latter could achieve only partial decolorization. These bacterial strains can potentially be applied to assess major types dyes in wastewater dye-polluted rivers. strain degraded Congo red its intermediates then further phenyl compounds. Interestingly, produced a...