Activation of both platelets and neutrophils can contribute to the risk major adverse cardiovascular events (MACE) following acute myocardial infarction (AMI). Neutrophil extracellular traps (NETs) are an important product platelet-neutrophil axis exaggerate vascular damage in disease. Additionally, activated drive NETosis directly linked thromboembolic risk. Investigating combined effect biomarkers for platelet activation represents a novel approach prediction post-AMI. Here, we examined...

This 21-marker, 18-color panel was developed to accurately delineate monocyte subsets by manual gating and, once successfully gated, characterize function in-depth. optimized on human peripheral blood mononuclear cells (PBMCs) density-separated from whole drawn into Cyto-Chex collection tubes (BCTs; Streck, La Vista, NE) healthy adults. All samples were analyzed a three-laser (violet-blue-red) Aurora full spectrum flow cytometer (Cytek Biosciences, Fremont, CA). Deep phenotyping achieved...

Many studies have shown that elevated biomarkers of inflammation following acute myocardial infarction (AMI) are associated with major adverse cardiovascular events (MACE). However, the optimal way measuring complex inflammatory response AMI has not been determined. In this study we explore use principal component analysis (PCA) utilising multiple cytokines to generate a combined cytokine score may be predictive MACE post-AMI. Thirteen were measured in plasma 317 patients, drawn 48–72 h...

Full spectrum flow cytometry (FSFC) allows for the analysis of more than 40 parameters at single-cell level. Compared to practice manual gating, high-dimensional data can be used fully explore datasets and reduce time. As panel size complexity increases so too does detail time required prepare validate quality resulting use in downstream analyses. To ensure algorithms efficiently avoid artifacts, some important steps should considered. These include cleaning (such as eliminating variable...

Platelets are important regulators of vascular thrombosis and inflammation known to express Toll-like receptors (TLRs). Through TLRs, platelets mediate a number responses by interacting with leucocytes. Here, we report the extent which modulate in vitro peripheral blood mononuclear cells (PBMCs) granulocyte TLR4, TLR2/1 TLR2/6 stimulation healthy subjects.Peripheral granulocytes from 10 volunteers were cultured alone or cocultured platelets. Cultures left unstimulated stimulated 1 100 ng...

Introduction In addition to their role in facilitating leukocyte-mediated inflammation, platelets can dampen leukocyte pro-inflammatory responses some contexts. Consequently, are increasingly appreciated as regulators of inflammation. Together, and neutrophils play a inflammation through Toll-like receptor (TLR) expression, although we do not fully understand how shape neutrophil TLR stimulation. Here, aimed determine the extent which modulate function response vitro stimulation with TLR4,...

Abstract EVs released by adipose derived stem cells (ADSCs) have shown promise as a therapeutic for tissue repair because of their purported immune‐regulatory properties. Extracellular vesicles (EVs) from ADSCs could be beneficial in improving graft retention rates autologous fat grafting (AFG) post‐mastectomy as, currently, grafted are variable. Enriching with ADSC‐EVs may improve modulating macrophages resident within both the breast and lipoaspirate. We aimed to identify key macrophage...

Abstract Extracellular matrix (ECM) biomarkers are useful for measuring underlying molecular activity associated with cardiac repair following acute myocardial infarction (AMI). The aim of this study was to conduct exploratory factor analysis (EFA) examine the interrelationships between ECM biomarkers, and cluster identify if distinct profiles could distinguish patient risk in AMI. Ten were measured from plasma 140 AMI patients: MMP-2, -3, -8, -9, periostin, procollagen I N-Terminal...

Incidence of endometrial cancer (EC) is rising in the developed world. The current standard care, hysterectomy, often infeasible for younger patients and those with high body mass index. There are limited non-surgical treatment options a lack biologically relevant research models to investigate novel alternatives surgery EC. aim present study was develop long-term, patient-derived explant (PDE) model early-stage EC demonstrate its use investigating predictive biomarkers option,...

Recent evidence suggests that neutrophils are highly plastic cells can display heterogeneous phenotypes. Low-density (LDNs) have been described in many inflammatory conditions, and thought to represent an immature, hyperactivated subtype of neutrophils. Neutrophils significantly involved the response myocardial infarction (MI), although we do not know extent which LDNs exist, or function, MI. This study sought determine frequency phenotype MI patients, compared healthy subjects (HS).LDNs...

Platelet activation underlies the pathology of an acute myocardial infarction (AMI), and dual antiplatelet therapy (DAPT) is administered post-AMI to limit this activation. Platelets express Toll-like receptors (TLRs) 1, 2, 4 become potently activated in response TLR2/1 TLR4 stimulation. However, it unknown whether agents can protect against platelet via these TLR pathways. This study aimed determine extent which TLR-mediated be inhibited by currently used agents. Ten healthy subjects were...

With the recent discovery of their ability to produce neutrophil extracellular traps (NETs), neutrophils are increasingly appreciated as active participants in infection and inflammation. NETs characterized large, web-like networks DNA proteins extruded from neutrophils, there is considerable interest how these structures drive disease humans. Advancing research this field contingent on developing novel tools for quantifying NETosis. To end, we have developed a 7-marker flow cytometry panel...

Introduction: Dual anti-platelet therapy (DAPT) is routinely administered to inhibit platelet aggregation in acute myocardial infarction (AMI) and, doing so, reduces the risk of recurrent adverse cardiovascular events. Alongside platelets, neutrophils are also key mediators inflammation during AMI, both secreting cytokines and chemokines producing Neutrophil Extracellular Traps (NETs). While DAPT known mediate off-target anti-inflammatory effects, extent which affects neutrophil function not...

**Background**: Autologous fat grafting (AFG) is an increasingly popular adjunct to post-mastectomy breast reconstruction as it can produce a natural cosmetic outcome and alleviate undesirable radiotherapy-associated consequences, with low surgical risk. However, graft retention rates are variable unpredictable. In order improve understand what influences rates, accurate consistent methods for the measurement of must be developed.**Methods**: Twenty patients who were undergoing AFG following...

Background: Patients with myocardial infarction (MI) are at an increased risk of experiencing recurrent major adverse cardiovascular events (MACE) but predicting MACE has remained challenging. Immunoglobulins implicated in disease, although the predictive value total immunoglobulin G (IgG) not yet been evaluated a secondary prevention setting. This study examined whether IgG is MI population, and how compared to C-reactive protein (CRP), acute inflammatory marker. Methods: We conducted...