A5039898990- Single-cell and spatial transcriptomics
- Immune Cell Function and Interaction
- Immune cells in cancer
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Photodynamic Therapy Research Studies
- Polyomavirus and related diseases
- Helicobacter pylori-related gastroenterology studies
- Multiple Myeloma Research and Treatments
- Medical and Biological Ozone Research
- Genetics, Aging, and Longevity in Model Organisms
- Reproductive Biology and Fertility
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Fluorescence Microscopy Techniques
- Hematological disorders and diagnostics
- Cardiac, Anesthesia and Surgical Outcomes
- Hematopoietic Stem Cell Transplantation
- Skin Protection and Aging
- Cutaneous lymphoproliferative disorders research
- CAR-T cell therapy research
- Microfluidic and Bio-sensing Technologies
- Immune Response and Inflammation
- Chemokine receptors and signaling
- Nail Diseases and Treatments
- Dermatology and Skin Diseases
South Australia Pathology
2011-2014
Hanson Institute
2012-2014
The University of Adelaide
2004
University of Limerick
1998
Nenagh Hospital
1998
OBJECTIVES: Immune activation may have an important pathogenic role in the irritable bowel syndrome (IBS). While little is known about immunologic function functional dyspepsia (FD), we observed association between cytokine secretion by peripheral blood mononuclear cells (PBMCs) and symptoms IBS. Upper gastrointestinal inflammatory diseases are characterized enhanced small homing α4-, β7-integrin, chemokine receptor 9 (CCR9) positive T lymphocytes. We hypothesized that increased release...
Multiple myeloma is an incurable hematologic cancer characterized by the clonal proliferation of malignant plasma cells within bone marrow. Numerous studies suggest that occupy and alter stromal tissue marrow as a means enhancing their survival growth. However, nature magnitude changes to cell remain be determined. In this study, we used mesenchymal osteoblast-related surface marker expression (STRO-1 alkaline phosphatase, respectively) flow cytometry enumerate osteoblast numbers in...
Abstract Technological advancements in fluorescence flow cytometry and an ever‐expanding understanding of the complexity immune system have led to development large panels reaching up 43 colors at single‐cell level. However, as panel size increase, so too does detail involved designing optimizing successful high‐quality fit for downstream high‐dimensional data analysis. In contrast conventional cytometers, full‐spectrum cytometers measure entire emission spectrum each fluorophore across all...
This 21-marker, 18-color panel was developed to accurately delineate monocyte subsets by manual gating and, once successfully gated, characterize function in-depth. optimized on human peripheral blood mononuclear cells (PBMCs) density-separated from whole drawn into Cyto-Chex collection tubes (BCTs; Streck, La Vista, NE) healthy adults. All samples were analyzed a three-laser (violet-blue-red) Aurora full spectrum flow cytometer (Cytek Biosciences, Fremont, CA). Deep phenotyping achieved...
Abstract Microglia are the innate myeloid cells of central nervous system (CNS) parenchyma, functionally implicated in almost every defined neuroinflammatory and neurodegenerative disorder. Current understanding disease pathogenesis for many neuropathologies is limited and/or lacks reliable diagnostic markers, vaccines, treatments. With increasing aging society rise neurogenerative diseases, improving our their essential. Analysis microglia from murine models provides an investigative tool...
Chemokines constitute a group of over 40 secreted peptides that are important for the control leukocyte migration both during homeostasis and inflammation. Recent studies have implicated ligands CCL19 CCL21 their receptor, CCR7, in specific naïve lymphocytes mature dendritic cells to secondary lymphoid organs immune homeostasis. However, role these molecules play priming is not well understood. In this study, using CCL19((8-83)), novel N-terminal truncation mutant, we investigated primary...
This study investigated the effects of high temperatures on male germ cell development and epididymal sperm motility laboratory mice. In Experiment 1, adult males (n=16) were exposed to whole-body heat 37-38°C for 8h day(-1) 3 consecutive days, whereas controls (n=4) left at 23-24°C. 2, mice (n=6) a single 8-h period with 2 was conducted as continuation previous that showed changes in spermatozoa 16h after exposure days. present study, temperature reduced testes weights 14 days exposure, by...
Despite the success of immune checkpoint blockade, most metastatic melanoma patients fail to respond therapy or experience severe toxicity. Assessment biomarkers and immunophenotypes before early into treatment will help understand favourable responses improve therapeutic outcomes.We present a high-dimensional approach for blood T-cell profiling using three multi-parameter cytometry panels: (1) TruCount panel absolute cell counts, (2) 27-colour spectral assessing markers (3) 20-colour...
Aims: Kidney Transplant Recipients (KTR) have an increased risk of both cutaneous squamous cell cancer (SCC) and non-lymphoid (NLC). High numbers regulatory T cells (Tregs, ie. CD3+CD4+FOXP3+CD25highCD127low) low Natural Killer (NK, CD3-CD19-CD56+CD16+) been shown to predict SCC development in KTR from the United Kingdom. These were utilising predominantly calcineurin (CNI) azathioprine (AZA) based immunosuppression regimens. This study investigated Treg NK immune phenotype Australian...