A5027273202- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- Cancer-related gene regulation
- Ubiquitin and proteasome pathways
- Enzyme function and inhibition
- Platelet Disorders and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Hemoglobin structure and function
- Immune Cell Function and Interaction
- Nitric Oxide and Endothelin Effects
- Soil Carbon and Nitrogen Dynamics
- Galectins and Cancer Biology
- Agronomic Practices and Intercropping Systems
- Mass Spectrometry Techniques and Applications
- Hedgehog Signaling Pathway Studies
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Heat shock proteins research
- Protein Structure and Dynamics
- Biochemical and Molecular Research
- Carbohydrate Chemistry and Synthesis
- Chemical Reactions and Mechanisms
- Pharmacological Effects and Assays
- Drug Transport and Resistance Mechanisms
- Protein Kinase Regulation and GTPase Signaling
Institute of Biomedical Sciences, Academia Sinica
2006-2024
National Yang Ming Chiao Tung University
2012-2014
Institute of Molecular Biology, Academia Sinica
2014
National Defense Medical Center
2014
National Yang Ming University Hospital
2014
Ming Chuan University
2014
North Carolina State University
2003-2014
University of Florida
1993-2012
National Cheng Kung University
2004-2009
Academia Sinica
2006
Core fucosylation (addition of fucose in α-1,6-linkage to core N-acetylglucosamine N-glycans) catalyzed by fucosyltransferase 8 (FUT8) is critical for signaling receptors involved many physiological and pathological processes such as cell growth, adhesion, tumor metastasis. Transforming growth factor-β (TGF-β)-induced epithelial-mesenchymal transition (EMT) regulates the invasion metastasis breast tumors. However, whether receptor affects TGF-β during cancer progression remains largely...
SCUBE1 (signal peptide-CUB-EGF domain-containing protein 1) is a novel, secreted, cell surface glycoprotein expressed during early embryogenesis and found in platelet endothelial cells. This composed of an N-terminal signal peptide sequence followed by nine tandemly arranged epidermal growth factor (EGF)-like repeats, spacer region, three cysteine-rich repeat motifs, one CUB domain at the C terminus. However, little known about its biological function. Here, we generated comprehensive panel...
The aim of the study was to investigate protein expression and function a novel secreted in vascular system, named SCUBE1 for signal peptide, CUB (Complement proteins C1r/C1s, Uegf, Bmp1) epidermal growth factor-like (EGF)-like domain containing 1.Immunohistochemical analysis demonstrated that staining is mainly confined intravascular platelet-rich thrombus tissue samples. While quantitative real-time RT-PCR verified mRNA expressed human platelets, numerous immunolocalization techniques...
Carbonic anhydrases (CAs) are ubiquitous enzymes that catalyze the reversible hydration/dehydration of carbon dioxide/bicarbonate. As such, there is enormous industrial interest in using CA as a bio-catalyst for sequestration and biofuel production. However, to ensure cost-effective use enzyme under harsh conditions, studies were initiated produce variants with enhanced thermostability while retaining high solubility catalytic activity. Kinetic structural conducted determine functional...
The emergence of castration-resistance is one the major challenges in management patients with advanced prostate cancer. Although spectrum systemic therapies that are available for use alongside androgen deprivation treatment castration-resistant cancer (CRPC) expanding, none these regimens curative. Therefore, it imperative to apply systems approaches identify and understand mechanisms contribute development CRPC. Using comprehensive proteomic approaches, we show a glycosylation-related...
A cDNA encoding the mouse carbonic anhydrase V gene was isolated by reverse transcription and polymerase chain reaction from BALB/c liver mRNA.Vectors containing full coding sequence as well two different NH,-terminal truncated genes expressed enzymatically active protein in Escherichia coli.The produced a vector sequence, which includes possible NH,terminal mitochondrial targeting signal, proteolytically processed E. coli contained several aminoterminal ends.The vectors deleted,...
The therapeutic efficacy of HER2/c-erbB-2/neu DNA immunization on mouse tumor cells expressing exogenous human or rat p185neu but not naturally has been demonstrated. We investigated the feasibility using N-terminal neu overexpressing endogenous and enhancing this vaccine by fusion to various cytokine genes, including interleukin-2 (IL-2), interleukin-4 (IL-4), granulocyte–macrophage colony-stimulating factor. In a model, N′-neu-IL-2 was significantly better than N′-neu vaccine, control...
Signal peptide-CUB-EGF domain-containing protein 1 (SCUBE1), a secreted and surface-exposed glycoprotein on activated platelets, promotes platelet-platelet interaction supports platelet-matrix adhesion. Its plasma level is biomarker of platelet activation in acute thrombotic diseases. However, the exact roles SCUBE1 vivo remain undefined.
Objective The aim of this study was to investigate in a transgenic animal model the cardiac expression and function novel extracellular protein SCUBE3 [signal peptide-CUB (complement proteins C1r/C1s, Uegf, Bmp1)-EGF (epidermal growth factor)-like domain-containing 3]. Methods results Real-time quantitative reverse transcriptase (RT)-PCR analysis showed that is expressed ventricular myocardium. Male (TG) mice overexpressing appeared normal during development, from birth adulthood. However,...
Abstract Background We recently showed that fucosyltransferase 8 (FUT8)-mediated core fucosylation of transforming growth factor-β receptor enhances its signaling and promotes breast cancer invasion metastasis. However, the complete FUT8 target glycoproteins their downstream networks critical for progression remain largely unknown. Method performed quantitative glycoproteomics with two highly invasive cell lines to unravel a comprehensive list core-fucosylated by comparison parental...
Tyrosine kinase inhibitor gefitinib is effective against lung cancer cells carrying mutant epidermal growth factor receptor (EGFR); however, it not normal EGFR. The breaking of immune tolerance self with active immunization may be a useful approach for the treatment EGFR-positive tumors. Xenogeneic EGFR gene was demonstrated to induce antigen-specific response EGFR-expressing tumor intramuscular administration.In order enhance therapeutic effect xenogeneic DNA vaccine, efficacy altering...
Geldanamycin (GA), a heat-shock protein (HSP) 90 inhibitor, induces degradation of HSP90 client proteins, which may promote the presentation peptides with major histocompatibility complex class I on cancer cells. We hypothesized that GA enhance efficacy DNA vaccination, and investigated therapeutic effect combination vaccine against clients p185neu Met. The various doses combined an N-terminal neu (N′-neu) was in transplanted tumor constitutively overexpressing endogenous p185neu. Low-dose...
The adverse effects of sepsis-associated acute kidney injury (SA-AKI) highlight the need for new biomarkers. Signal Peptide-Complement C1r/C1s, Uegf, Bmp1-Epidermal Growth Factor-like Domain-Containing Protein 2 (SCUBE2), important angiogenesis and endothelial integrity, has been linked to increased mortality in models lipopolysaccharide-induced lung injury. This research aimed assess utility plasma SCUBE2 levels as a prognostic indicator SA-AKI intensive care unit (ICU) patients.
We recently identified a novel testis-enriched receptor guanylyl cyclase (GC) in the mouse, designated mGC-G. To further investigate its protein expression and function, we generated neutralizing antibody specifically against extracellular domain of this receptor. RT-PCR immunohistochemical analyses show that mGC-G is predominantly expressed from round spermatids to spermatozoa mouse testis at both mRNA levels. Flow cytometry confocal immunofluorescence reveal cell surface restricted plasma...
Abstract The replacement His107-->Tyr is a cause of carbonic anhydrase II deficiency syndrome in humans (Venta, P. J., Welty, R. Johnson, T. M., Sly, W. S., and Tashian, E. (1991) Am. J. Hum. Genet. 49, 1082-1090). We have prepared this mutant human by site-directed mutagenesis expressed it Escherichia coli. was too unstable to purify; however, we were able stabilize store at 4 degrees C cell lysates containing 1-4 mg/ml bovine serum albumin. concentration the lysate determined titration...