A5052476474- Neuroscience of respiration and sleep
- Infant Health and Development
- Neuroendocrine regulation and behavior
- Sleep and Wakefulness Research
- Heart Rate Variability and Autonomic Control
- Obstructive Sleep Apnea Research
- Neurological Disease Mechanisms and Treatments
- Botulinum Toxin and Related Neurological Disorders
- Biochemical Analysis and Sensing Techniques
- Acute Ischemic Stroke Management
- Welding Techniques and Residual Stresses
- Metal Alloys Wear and Properties
- Neonatal Respiratory Health Research
- Medical Imaging and Analysis
- Microstructure and Mechanical Properties of Steels
- Child Nutrition and Feeding Issues
- Additive Manufacturing Materials and Processes
- Neuroinflammation and Neurodegeneration Mechanisms
- Cervical Cancer and HPV Research
- Magnesium Alloys: Properties and Applications
- Dysphagia Assessment and Management
- Advanced Welding Techniques Analysis
- Immune Response and Inflammation
- Cerebrovascular and Carotid Artery Diseases
- Autophagy in Disease and Therapy
Macquarie University
2008-2024
First Affiliated Hospital of Kunming Medical University
2023
Kunming Medical University
2023
Jilin University
2022
Hebei North University
2021
China Medical University
2018-2021
Beijing Union University
2020
Nanjing Drum Tower Hospital
2020
Third People's Hospital of Huzhou
2019-2020
University of Shanghai for Science and Technology
2019
Breathing results from sequential recruitment of muscles in the expiratory, inspiratory, and postinspiratory (post-I) phases respiratory cycle. Here we investigate whether neurons medullary intermediate reticular nucleus (IRt) are components a central pattern generator (CPG) that generates post-I activity laryngeal adductors vasomotor sympathetic nerves interacts with other members network to terminate inspiration. We first identified region (male) rat IRt contains highest density lightly...
Abstract The extent of the adrenergic input to respiratory neurons in ventrolateral medulla oblongata rats was assessed by using a combination intracellular recording, dye filling, and immunohistochemistry. Twenty‐two that displayed pronounced respiration‐related modulation their membrane potential, could not be antidromically activated electrical stimulation superior laryngeal, vagus, or facial nerves, were labelled injection biocytin. Three types labelled: small located Bötzinger complex...
Barosensitive neurons in the rostral ventrolateral medulla (RVLM) often have a respiratory-related modulation of their activity. However, extent interaction between baroreceptor and respiratory inputs is controversial. The main aim present study was to determine effect central drive (CRD) on barosensitivity RVLM neurons. Extracellular recordings were obtained from 68 barosensitive anesthetized, paralyzed, bilaterally vagotomized Sprague-Dawley rats. Examination phrenic-triggered histograms...
Gelsemium elegans Benth. (G. elegans), which is a famous Chinese folk medicine, has been commonly used to treat certain types of skin ulcers and alleviate inflammation, headaches, cancer pain. However, the extensive clinical use G. greatly hampered by its toxicity. As one most widely herbal medicines, Glycyrrhiza uralensis Fisch, unique effect on detoxification elegans. In present study, rapid sensitive method using ultra-liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was...
Sympathetic nerve activity often fluctuates with the respiratory cycle, but central neurons that impose this modulation have not been conclusively identified. In present study, we used intracellular recording and dye-filling to identify expiratory in Bötzinger complex. Our aim was see if project towards putative cardiovascular rostral ventrolateral medulla. first series of experiments, histochemistry immunohistochemistry were reveal labelled immunoreactive for tyrosine hydroxylase. Two out...
A striking effect of stimulating the superior laryngeal nerve (SLN) is its ability to inhibit central inspiratory activity (cause ‘phrenic apnoea'), but mechanism underlying this inhibition remains unclear. Here we demonstrate, by SLN at varying frequencies, that evoked non-respiratory burst recorded from expiratory motoneurons (ELMs) has an intimate temporal relationship with phrenic apnoea. During 1–5 Hz stimulation, occasional absences discharge (PND) occurred such every absent PND was...
The effect of angiotensin II type I receptor (AT1R) inhibition on the pattern reflex sympathetic nerve activity (SNA) to multiple target organs in Lewis polycystic kidney (LPK) rat model chronic disease was determined.Mean arterial pressure (MAP), splanchnic SNA (sSNA), renal (rSNA) and lumbar (lSNA) were recorded urethane-anaesthetized LPK controls (total n = 39). Baroreflex, peripheral central chemoreflex, somatosensory control (evoked by phenylephrine/sodium nitroprusside infusion, 10% O2...
Inhibition of neurons in the pontine Kölliker–Fuse/parabrachial complex (KF-PBN) differentially inhibited postinspiratory (post-I) activity vagal and sympathetic outputs. The strong recruitment post-I that occurs response to hypoxemia is selectively abolished by KF-PBN inhibition. This suggests 1) outputs may be generated partially independent mechanisms 2) are a preeminent source drive for generation eupneic activity.
Abstract Serotonergic neurons are distributed widely throughout the central nervous system and exert a tonic influence on range of activities in relation to sleep–wake cycle. Previous morphologic functional studies have indicated role for serotonin control laryngeal motoneurons. In present study, we used combination intracellular recording, dye‐filling, immunocytochemistry rats demonstrate close appositions between immunoreactive boutons posterior cricoarytenoid (PCA) cricothyroid (CT)...
Objectives: Electromyographic studies of the laryngeal adductor reflex, glottal closure occurring in response to stimulation, have demonstrated an early ipsilateral (R1) and a late bilateral (R2). To better define physiologic properties these responses, we recorded responses from expiratory motoneurons (ELMs) rats during stimulation superior nerve (SLN). Methods: Single unit extracellular recordings were obtained 5 ELMs, identified by their antidromic recurrent postinspiratory firing...
Genetic tools that permit functional or connectomic analysis of neuronal circuits are rapidly transforming neuroscience. The key to deployment such is selective transfection target neurons, but date this has largely been achieved using transgenic animals viral vectors transduce subpopulations cells chosen according anatomical rather than criteria. Here, we combine single-cell with conventional electrophysiological recording techniques, resulting in three novel protocols can be used for...
Calbindin-D28k (calbindin) is a calcium-binding protein that distributed widely in the rat brain. The localisation of calbindin immunoreactivity medulla oblongata and its colocalisation with adrenaline-synthesising neurons [phenylethanolamine-N-methyltransferase-immunoreactive (PNMT-IR)] was examined (Granata Chang [1994] Brain Res. 645:265–277). However, detailed information about distribution calbindin-IR reticular formation particular lacking. In this report, authors address issue an...
Abstract In the present study, we assessed extent of thyrotropin‐releasing hormone (TRH) input to motoneurons in ambigual, facial, and hypoglossal nuclei rat using a combination intracellular recording, dye filling, immunohistochemistry. Twelve rostral nucleus ambiguus were labelled by injection vivo Neurobiotin (Vector). Seven out 12 ambigual displayed rhythmic fluctuations their membrane potential phase with phrenic nerve discharge, whereas other five had no modulations any kind. facial...
The intrinsic laryngeal muscles are differentially modulated during respiration as well other states and behaviors such hypocapnia sleep. Previous anatomical pharmacological studies indicate a role for acetylcholine at the level of nucleus ambiguus in modulation motoneuron (LMN) activity. present study investigated nature cholinergic input to inspiratory- (ILM) expiratory-modulated (ELM) motoneurons loose formation ambiguus. Using combined vivo intracellular recording, dye filling,...
Objectives To explore the rat caudal raphe nuclei for neurons that respond to activation of baroreceptor nerves and have a spinal axon, compare behavioural properties barosensitive bulbospinal in with rostral ventrolateral medulla. Design Extracellular unit recordings were obtained from an area extending up 1.0 mm caudally edge facial nucleus. Two sites explored: medulla midline. Materials methods Single-unit made anaesthetized (75 mg/kg chloral hydrate 30 sodium pentobarbitone then 3–6 mg...
Abstract Objectives/Hypothesis: The objective was to demonstrate close appositions between serotonin‐immunoreactive boutons and laryngeal constrictor (LCon) motoneurons in Sprague‐Dawley rats. Study Design: Animal experimental. Methods: LCon were identified functionally by their antidromic responses stimulation of the recurrent nerve postinspiratory modulation filled intracellular injection biotin amide (n = 6). medulla sectioned and, using immunohistochemical analysis, examined light...