A5036169268- Obesity, Physical Activity, Diet
- Food Security and Health in Diverse Populations
- Diverse Educational Innovations Studies
- Urban Agriculture and Sustainability
- Child Nutrition and Water Access
- Dietetics, Nutrition, and Education
- Carcinogens and Genotoxicity Assessment
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Homelessness and Social Issues
- Health Policy Implementation Science
- Library Science and Administration
- Community Health and Development
- Chromium effects and bioremediation
- Advanced biosensing and bioanalysis techniques
- Nutrition, Health and Food Behavior
- Nutritional Studies and Diet
- Food Waste Reduction and Sustainability
- Impact of Technology on Adolescents
- Telomeres, Telomerase, and Senescence
- Child Development and Digital Technology
- Bacteriophages and microbial interactions
- Genomic variations and chromosomal abnormalities
- Environmental Education and Sustainability
- Obesity and Health Practices
University of Illinois Urbana-Champaign
2015-2024
University of Wisconsin–Extension
2014-2023
Goodwin College
2021
Drexel University
2015-2020
University of Illinois System
2019
City of Knowledge
2019
Saint Joseph Seminary College
2019
University of Kansas Medical Center
2019
University of Aberdeen
2014
University of Illinois Chicago
2006-2012
Abstract Large structural variants (SVs) in the human genome are difficult to detect and study by conventional sequencing technologies. With long-range analysis platforms, such as optical mapping, one can identify large SVs (>2 kb) across experiment. Analyzing maps of 154 individuals from 26 populations sequenced 1000 Genomes Project, we find that phylogenetic population patterns similar those single nucleotide variations 86% genome, while ~2% has high complexity. We able characterize...
Methylthioninium (MT) is a tau aggregation inhibitor with therapeutic potential in Alzheimer's disease (AD). MT exists equilibrium between reduced [leucomethylthioninium (LMT)] and oxidized (MT<sup>+</sup>) forms; as chloride salt [methylthioninium (MTC), "methylene blue"], it stabilized its MT<sup>+</sup> form. Although the results of phase 2 study MTC 321 mild/moderate AD subjects identified 138-mg MT/day dose minimum effective on cognitive imaging end points, further clinical development...
Certain forms of the heavy metals arsenic and chromium are considered human carcinogens, although they believed to act through very different mechanisms. Chromium(VI) is as a classic mutagenic agent, DNA/chromatin appears be principal target for its effects. In contrast, arsenic(III) nongenotoxic, but able specific cellular proteins, principally sulfhydryl interactions. We had previously shown that various genotoxic chemical including (VI), preferentially altered expression several inducible...
We have developed a new, sequence-specific DNA labeling strategy that will dramatically improve mapping in complex and structurally variant genomic regions, as well facilitate high-throughput automated whole-genome mapping. The method uses the Cas9 D10A protein, which contains nuclease disabling mutation one of two domains Cas9, to create guide RNA-directed nick context an vitro-assembled CRISPR-CAS9-DNA complex. Fluorescent nucleotides are then incorporated adjacent nicking site with...
We have developed a novel method that enables global subtelomere and haplotype-resolved analysis of telomere lengths at the single-molecule level. An in vitro CRISPR/Cas9 RNA-directed nickase system directs specific labeling human (TTAGGG)n DNA tracts genomes also been barcoded using separate enzyme recognizes 7-bp motif genome-wide. High-throughput imaging large single molecules from labeled this fashion nanochannel array permits mapping through repeat element (SRE) regions to unique...
Abstract Objective The present study examined plain water consumption in relation to energy intake and diet quality among US adults. Methods A nationally representative sample of 18 311 adults aged ≥18 years, from the National Health Nutrition Examination Survey 2005–2012, was analysed. first‐difference estimator approach addressed confounding bias time‐invariant unobservables (e.g. eating habits, taste preferences) by using within‐individual variations between two nonconsecutive 24‐h...
Escherichia coli C forms more robust biofilms than other laboratory strains. Biofilm formation and cell aggregation under a high shear force depend on temperature salt concentrations. It is the last of five E. strains (C, K12, B, W, Crooks) designated as safe for purposes whose genome has not been sequenced.
The charitable food system distributes free to clients across the U.S., but many nutrition and health-focused efforts encounter barriers success, which were exacerbated during COVID-19 pandemic. objective of current study was understand facilitators distributing nutritious, fresh foods in pantries Illinois pandemic.Forty-nine pantry representatives participated focus groups October 2021. A codebook created based on relevant literature, stakeholder interests, an initial review recordings....
Complexes between the retinoblastoma protein (pRb) and transcription factor E2F-1 are thought to be important for regulating cell proliferation. We have shown previously that E7 oncoprotein from human papillomavirus type 16, dependent upon its binding pRb proteins, induces proliferation, disrupts differentiation, apoptosis when expressed in differentiating, or fiber, cells of ocular lenses transgenic mice. Mice carry a null mutation inE2F-1 do not exhibit any defects proliferation...
Accurate maps and DNA sequences for human subtelomere regions, along with detailed knowledge of variation long-range telomere-terminal haplotypes in individuals, are critical understanding telomere function its roles biology. Here, we use a highly automated whole genome mapping technology nano-channel arrays to analyze large terminal chromosome segments extending from chromosome-specific through subtelomeric repeat regions (TTAGGG)n tracts. We establish gap the reference sequence, detect...
Nudge interventions are widely used to promote health in schools, yet implementation metrics seldom understand intervention outcomes. A multi-component consisting of cafeteria decorations, creative names, social norming taste tests, and flavor station components was implemented three rural elementary school cafeterias by nutrition services (SNS) extension staff. Selection consumption fruits vegetables at lunch were measured through monthly plate waste assessments over eight months (n = 1255...
Abstract Objective: The main objective of this study was to evaluate the impact Market MyPlate (M2MP) program on fruit and vegetable consumption cooking behaviours. Secondary objectives were examine factors that affected participant retention completion, analyse feedback provided by participants. Design: This conducted a mixed methods evaluation embedded within cluster randomised controlled trial M2MP intervention. Adult participants completed pre- post-program survey reporting their A...
Sequences encoding Olduvai protein domains (formerly DUF1220) show the greatest human lineage-specific increase in copy number of any coding region genome and have been associated, a dosage-dependent manner, with brain size, cognitive aptitude, autism, schizophrenia. Tandem intragenic duplications three-domain block, termed triplet, four
Whole-genome mapping technologies have been developed as a complementary tool to provide scaffolds for genome assembly and structural variation analysis (1,2). We recently introduced novel DNA labeling strategy based on CRISPR-Cas9 editing system, which can target any 20bp sequences. The is specifically useful in targeting repetitive sequences, sequences not accessible other methods. In this report, we present customized strategies that extend the applications of labeling. first design...
There is a need to disseminate evidence-based childhood obesity prevention interventions on broader scale reduce obesity-related disparities among underserved children. The purpose of this study was test the comparative effectiveness an intervention, Hip-Hop Health (HH), delivered through Expanded Food and Nutrition Education Program (EFNEP) Supplemental Assistance Program-Education (SNAP-Ed) versus standard curriculum by programs (Standard (NE)). A nonequivalent control group design compare...
Certain forms of the heavy metals arsenic and chromium are considered human carcinogens, although they believed to act through very different mechanisms.Chromium(VI) is as a classic genotoxic mutagenic agent, DNANchromatin appears be principal target for its effects.In contrast, arsenic(lll) nongenotoxic, but able specific cellular proteins, principally sulfhydryl interactions.We had previously shown that various chemical including chromium(VI), preferentially altered expression several...
Abstract Objective: The objective of the current study was to evaluate impact Market MyPlate (M2MP) program on participants’ reported farmers’ market (FM) attitudes and shopping behaviours, frequency serving vegetables their families, food resource management behaviours security. A secondary identify facilitators barriers at FM waste reduction techniques used by low-income families. Design: a mixed methods evaluation embedded within cluster randomised trial M2MP intervention. Setting: 7-week...
Abstract Previous studies have shown that a number of different genotoxic carcinogens induce types DNA damage preferentially alter the expression inducible genes in vivo. To investigate further mechanistic basis for these effects, we examined effects human lung carcinogen chromium(VI) on hormone‐inducible cytosolic phosphoenolpyruvate carboxykinase (PEPCK) gene chick embryo liver. Chromium(VI) pretreatment had significant both basal and glucocorticoid‐inducible PEPCK 14‐d‐old These were...
Abstract Background Telomeric DNA is typically comprised of G-rich tandem repeat motifs and maintained by telomerase (Greider CW, Blackburn EH; Cell 51:887–898; 1987). In eukaryotes lacking telomerase, a variety repair recombination based pathways for telomere maintenance have evolved in organisms normally dependent upon elongation (Webb CJ, Wu Y, Zakian VA; Cold Spring Harb Perspect Biol 5:a012666; 2013); collectively called Alternative Lengthening Telomeres (ALT) pathways. By measuring...