A5020701986- Epigenetics and DNA Methylation
- DNA and Nucleic Acid Chemistry
- RNA modifications and cancer
- Immune responses and vaccinations
- Birth, Development, and Health
- Prenatal Screening and Diagnostics
- DNA Repair Mechanisms
- Telomeres, Telomerase, and Senescence
- Advanced biosensing and bioanalysis techniques
- Genomic variations and chromosomal abnormalities
- Child Nutrition and Feeding Issues
- Genetic Syndromes and Imprinting
- Diet and metabolism studies
- Attention Deficit Hyperactivity Disorder
- Folate and B Vitamins Research
- Gut microbiota and health
- Genetics and Neurodevelopmental Disorders
- Immune Cell Function and Interaction
- Genomics and Chromatin Dynamics
- Plant and Fungal Interactions Research
- Microtubule and mitosis dynamics
- Chromosomal and Genetic Variations
- Genomics, phytochemicals, and oxidative stress
- Dental Health and Care Utilization
- RNA and protein synthesis mechanisms
University of Otago
2016-2025
University of Canterbury
2016
Abstract It has been widely hypothesized that both diet and the microbiome play a role in regulation of attention-deficit/hyperactivity disorder (ADHD) behaviour. However, there very limited scientific investigation into potential biological connection. We performed 10-week pilot study investigating effects broad spectrum micronutrient administration on faecal content, using 16S rRNA gene sequencing. The consisted 17 children (seven placebo ten treatment group) between ages seven 12 years,...
Abstract Background Environmental factors, such as oxidative stress, have the potential to modify epigenetic landscape of cells. We previously shown that DNA methyltransferase (DNMT) activity can be inhibited by sublethal doses hydrogen peroxide (H 2 O ). However, site-specific changes in methylation and reversibility any not been explored. Using bead chip array technology, differential was assessed Jurkat T-lymphoma cells following exposure H . Results Sublethal associated with an initial...
We observed apparent non-Mendelian behaviour of alleles when genotyping a region in CpG island at the 5′ end maternally imprinted human MEST isoform. This contains three single nucleotide polymorphisms (SNPs) total linkage disequilibrium, such that only two haplotypes occur population. Only one haplotype was detectable each subject, never both, despite use multiple primers and several methods. this motifs capable forming G-quadruplex structures. Circular dichroism spectroscopy native...
Sequences encoding Olduvai protein domains (formerly DUF1220) show the greatest human lineage-specific increase in copy number of any coding region genome and have been associated, a dosage-dependent manner, with brain size, cognitive aptitude, autism, schizophrenia. Tandem intragenic duplications three-domain block, termed triplet, four
Loss of one allele during polymerase chain reaction (PCR) amplification DNA, known as allelic dropout, can be caused by a variety mechanisms. Allelic dropout PCR may have profound implications for molecular diagnostic and research procedures that depend on assume biallelic has occurred. Complete due to the combined effects cytosine methylation G-quadruplex formation was previously described differentially methylated region human imprinted gene, MEST We now demonstrate this parent-of-origin...
Exposure times and dosage required for dietary components to modify DNA methylation patterns are largely unknown. Aim: This exploratory research represents the first genome-wide analysis of changes during a randomized-controlled-trial (RCT) supplementation with broad spectrum vitamins, minerals amino acids in humans. Methods: Genome-wide from paired, peripheral blood samples were assessed using Infinium Methylation EPIC 850 K array. Results: increased at 84% most significant differentially...
Oxidative stress is a common feature of inflammation-driven cancers, and it promotes genomic instability aggressive tumour phenotypes. It known that oxidative transiently modulates gene expression through the oxidation transcription factors associated regulatory proteins. Neutrophils are our most abundant white blood cells accumulate at sites infection inflammation. Activated neutrophils produce hypochlorous acid chloramines, which can disrupt DNA methylation by oxidizing methionine. The...
Interest in exploring G-quadruplex (G4) structures nucleic acids is growing as it becomes more widely recognized that these have many interesting biological roles and chemical properties. Probing the G4-forming potential of DNA with dimethyl sulfate, polymerase stop assays, or nuclease digestion are three commonly used techniques usually employ radio-isotopic labels for visualization. However, fluorescent labeling methods grown popularity versatility, laboratories moved away from routine use...
The promoter region of the imprinted gene MEST contains several motifs capable forming G-quadruplex (G4) structures, which appear to contribute consistent allelic dropout during polymerase chain reaction (PCR) analysis this region. Here, we extend our previous G4 structures by applying fluorescent footprinting techniques assess non B-DNA structure and topology in dsDNA from full region, under conditions that mimic PCR. We demonstrate buffer used for PCR provides an extremely favourable...
The promoter of the human imprinted gene MEST is differentially methylated with respect to parent origin and contains several non B-DNA motifs that are capable forming G-quadruplexes. These factors can contribute a consistent allelic dropout (ADO) maternally DNA during polymerase chain reaction (PCR) analysis such regions. Here, we directly investigate cause by applying fluorescent techniques visualize amplification arrest PCR templates. We demonstrate corresponds previously characterized...
This research investigates the intricate dynamics of DNA methylation in hours following CD8+ T cell activation, during a critical yet understudied temporal window. is an epigenetic modification central to regulation gene expression and directing immune responses. Our investigation spanned 96-h post-activation unveils nuanced tapestry global site-specific changes. We identified 15,626 significant differentially methylated CpGs spread across genome, with most changes occurring within genes...