A5057409778- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Cervical Cancer and HPV Research
- Advanced Biosensing Techniques and Applications
- Nanoparticle-Based Drug Delivery
- SARS-CoV-2 detection and testing
- ATP Synthase and ATPases Research
- CAR-T cell therapy research
- Phagocytosis and Immune Regulation
- Hepatitis B Virus Studies
- Mitochondrial Function and Pathology
- SARS-CoV-2 and COVID-19 Research
- Analytical Chemistry and Sensors
- Advanced biosensing and bioanalysis techniques
- Biochemical and Structural Characterization
- NF-κB Signaling Pathways
- Photosynthetic Processes and Mechanisms
- Virus-based gene therapy research
- Receptor Mechanisms and Signaling
- Cancer-related Molecular Pathways
- RNA and protein synthesis mechanisms
- Inhalation and Respiratory Drug Delivery
University of Tübingen
2020-2024
Natural and Medical Sciences Institute
2021-2023
Pharmaceutical Biotechnology (Czechia)
2022-2023
Institute of Medical Microbiology and Hygiene
2023
The ongoing COVID-19 pandemic and the emergence of new SARS-CoV-2 variants concern (VOCs) requires continued development effective therapeutics. Recently, we identified high-affinity neutralizing nanobodies (Nbs) specific for receptor-binding domain (RBD) SARS-CoV-2. Taking advantage detailed epitope mapping, generate two biparatopic Nbs (bipNbs) targeting a conserved outside different epitopes inside RBD:ACE2 interface. Both bipNbs bind all currently circulating VOCs with high affinities...
Signal-regulatory protein α (SIRPα) expressed by myeloid cells is of particular interest for therapeutic strategies targeting the interaction between SIRPα and “don’t eat me” ligand CD47 as a marker to monitor macrophage infiltration into tumor lesions. To address both approaches, we developed set novel human (hSIRPα)–specific nanobodies (Nbs). We identified high-affinity Nbs hSIRPα/hCD47 interface, thereby enhancing antibody-dependent cellular phagocytosis. For non-invasive in vivo imaging,...
The degradation of p53 is a hallmark high-risk human papillomaviruses (HPVs) the alpha genus and HPV-related carcinogenicity. oncoprotein E6 forms ternary complex with E3 ubiquitin ligase E6-associated protein (E6AP) tumor suppressor targeting for ubiquitination. extent by different proteins varies greatly, even closely related HPV16 HPV31. HPV31 display high sequence identity (∼67%). We report here, first time, structure bound to LxxLL motif E6AP. are structurally very similar, in agreement...
Purpose Human OX40 (hOX40/CD134), a member of the TNF receptor superfamily, is mainly expressed on activated T lymphocytes. Triggered by its ligand OX40L (CD252), it provides costimulatory signals that support differentiation, proliferation and long-term survival cells. Besides being relevant therapeutic target, hOX40 also an important biomarker for monitoring presence or infiltration cells within tumor microenvironment (TME), inflammatory (IME) in immune-mediated diseases (IMIDs) lymphatic...
Human papillomaviruses (HPVs) are DNA tumor viruses that infect mucosal and cutaneous epithelial cells of more than 20 vertebrates. High-risk HPV causes about 5% human cancers worldwide, the viral proteins E6 E7 promote carcinogenesis by interacting with suppressors interfering many cellular pathways. As a consequence, they immortalize efficiently in concert individually. So far, networks their respective targets have been studied extensively but independently. However, we hypothesized might...
In cells, mitochondria undergo constant fusion and fission. An essential factor for fission is the mammalian dynamin-related protein 1 (Drp1). Dysregulation of Drp1 associated with neurodegenerative diseases including Parkinson's, cardiovascular cancer, making a pivotal biomarker monitoring mitochondrial status potential pathophysiological conditions. Here, we developed nanobodies (Nbs) as versatile binding molecules proteomics, advanced microscopy live cell imaging Drp1. To specifically...
Abstract Purpose Human OX40 (hOX40/CD134), a member of the TNF receptor superfamily, is mainly expressed on activated T lymphocytes. Triggered by its ligand OX40L (CD252), it provides costimulatory signals that support differentiation, proliferation and long-term survival cells. Besides being relevant therapeutic target, hOX40 also an important biomarker for monitoring presence or infiltration cells within tumor microenvironment (TME), inflammatory (IME) in immune-mediated diseases (IMIDs)...
Abstract The ongoing COVID-19 pandemic and the frequent emergence of new SARS-CoV-2 variants concern (VOCs), requires continued development fast effective therapeutics. Recently, we identified high-affinity neutralizing nanobodies (Nb) specific for receptor-binding domain (RBD) SARS-CoV-2, which are now being used as biparatopic Nbs (bipNbs) to investigate their potential future drug candidates. Following detailed in vitro characterization, chose NM1267 most promising candidate showing high...
Abstract Signal-regulatory protein α (SIRPα) expressed by myeloid cells is of particular interest for therapeutic strategies targeting the interaction between SIRPα and "don’t eat me" ligand CD47 as a marker to monitor macrophage infiltration into tumor lesions. To address both approaches, we developed set novel human (hSIRPα)-specific nanobodies (Nbs). We identified three high-affinity Nbs hSIRPα/hCD47 interface, thereby enhancing antibody-dependent cellular phagocytosis (ADCP). For...
Abstract In cells, mitochondria undergo constant fusion and fission. An essential factor for fission is the mammalian dynamin-related protein 1 (Drp1). Dysregulation of Drp1 has been linked to neurodegenerative diseases including Parkinson’s as well cardiovascular cancer. Here, we developed nanobodies (Nbs) proteomics, advanced microscopy live cell imaging Drp1. To specifically enrich endogenous with interacting proteins functionalized high-affinity Nbs capture matrices. Furthermore,...
ABSTRACT The tumor microenvironment (TME) consists of different cell types that secrete proteins and also control the extracellular concentration ions metabolites. Changes in these intra-tumoral analytes conditions, including K + , glucose, pH, have been described to alter metabolic activity cancer cells, promote growth, impair anti-tumor immunity. However, mechanisms regulating ion metabolite levels their effects on certain characteristics TME are still poorly understood. Therefore,...
The tumor microenvironment (TME) consists of different cell types that secrete proteins and also control the extracellular concentration ions metabolites. Changes in these intra-tumoral analytes conditions, including K+, glucose, pH, have been described to alter metabolic activity cancer cells, promote growth, impair anti-tumor immunity. However, mechanisms regulating ion metabolite levels their effects on certain characteristics TME are still poorly understood. Therefore, accurate...