A5024909409- Viral Infections and Immunology Research
- interferon and immune responses
- Cardiac Fibrosis and Remodeling
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Cytomegalovirus and herpesvirus research
- Viral gastroenteritis research and epidemiology
- T-cell and B-cell Immunology
- Influenza Virus Research Studies
- Cancer Immunotherapy and Biomarkers
- Parvovirus B19 Infection Studies
- Cardiomyopathy and Myosin Studies
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- Cardiovascular Effects of Exercise
- Ion Transport and Channel Regulation
- Advanced MRI Techniques and Applications
- vaccines and immunoinformatics approaches
- Pancreatic function and diabetes
- Respiratory viral infections research
- Cardiac Imaging and Diagnostics
- Systemic Sclerosis and Related Diseases
- Bladder and Urothelial Cancer Treatments
- Metabolism, Diabetes, and Cancer
University of Tübingen
2006-2024
University Children's Hospital Tübingen
2007-2023
TH Bingen University of Applied Sciences
2023
Universitätsklinikum Tübingen
2004-2017
Stiftung für Forschung in Spätantike und Mittelalter HR. Sennhauser
2017
University of Zurich
2017
Charité - Universitätsmedizin Berlin
2014
Johns Hopkins University
2014
German Centre for Cardiovascular Research
2014
Bayer (Germany)
2009
Influenza A viruses are a threat to humans due their ability cross species barriers, as illustrated by the 2009 H1N1v pandemic and sporadic H5N1 transmissions. Interspecies transmission requires adaptation of viral polymerase importin-α, cellular protein that mediates transport into nucleus where transcription replication genome takes place. In this study, we analysed replication, host specificity pathogenicity avian mammalian influenza viruses, in importin-α-silenced cells...
Transforming growth factor β (TGF-β) has been shown to participate in the pathophysiology of diabetic complications. As most recently, TGF-β stimulates expression a distinct serine/threonine kinase (hSGK) which had previously cloned as an early gene transcriptionally regulated by cell volume alterations. The present study was performed elucidate transcription and function hSGK nephropathy. Northern blotting, increase extracellular glucose concentration increased mRNA levels cultured cells,...
Inflammation is a major factor in heart disease. IκB kinase (IKK) and its downstream target NF-κB are regulators of inflammation activated cardiac disorders, but their precise contributions targets unclear. We analyzed IKK/NF-κB function the by gain-of-function approach, generating an inducible transgenic mouse model with cardiomyocyte-specific expression constitutively active IKK2. In adult animals, IKK2 activation led to inflammatory dilated cardiomyopathy failure. Transgenic hearts showed...
Background: Immune checkpoint inhibitor (ICI) therapy is often accompanied by immune-related pathology, with an increasing occurrence of high-risk ICI-related myocarditis. Understanding the mechanisms involved in this side effect could enable development management strategies. In mouse models, immune checkpoints, such as PD-1 (programmed cell death protein 1), control threshold self-antigen responses directed against cardiac TnI (troponin I). We aimed to identify how immunoproteasome, main...
In order to identify organ and cellular targets of persistent enterovirus infection in vivo, immunocompetent mice (SWR/J, H-2q) were inoculated intraperitoneally with coxsackievirus B3 (CVB3). By use situ hybridization for the detection enteroviral RNA, we show that CVB3 is capable inducing a multiorgan disease. During acute infection, viral RNA was visualized at high levels heart muscle, pancreas, spleen, lymph nodes comparably low central nervous system, thymus, lung, liver. At later...
Background— Common causative agents in the development of inflammatory cardiomyopathy include cardiotropic viruses such as coxsackievirus B3 (CVB3). Here, we investigated role ubiquitin-like modifier interferon-stimulated gene 15 kDa (ISG15) pathogenesis viral cardiomyopathy. Methods and Results— In CVB3-infected mice, absence protein modification with ISG15 was accompanied by a profound exacerbation myocarditis significant increase mortality heart failure. We found that cardiomyocytes...
Proteasomes recognize and degrade poly-ubiquitinylated proteins. In infectious disease, cells activated by interferons (IFNs) express three unique catalytic subunits β1i/LMP2, β2i/MECL-1 β5i/LMP7 forming an alternative proteasome isoform, the immunoproteasome (IP). The in vivo function of IPs pathogen-induced inflammation is still a matter controversy. were mainly associated with MHC class I antigen processing. However, recent findings pointed to more general response cytokine stress. Here,...
The characteristics of dilated cardiomyopathy (DCM) resulting from chronic viral myocarditis are remodeling processes the extracellular matrix. Based on our findings enhanced osteopontin (OPN) expression in inflamed human hearts, we further investigated murine model acute and coxsackievirus (CV)B3-myocarditis role OPN regarding its involvement resolution cardiac virus infection fibrosis. In hearts A.BY/SnJ mice susceptible to CVB3-myocarditis, a pronounced increase levels was detected by...
Background Diagnosis of viral myocarditis is difficult by clinical criteria but facilitated detection inflammation and genomes in endomyocardial biopsies. Parvovirus B19 (B19V) targets endothelial cells where nucleic acid exclusively detected the heart. Microparticles (MPs) are released after cell damage or activation specific cells. We aimed to investigate whether circulating MPs (EMPs) human experimental models associated with B19V myocarditis. Methods were investigated patients (n = 54),...
Enteroviruses such as coxsackievirus B3 (CVB3) are able to induce lethal acute and chronic myocarditis. In resistant C57BL/6 mice, CVB3 myocarditis is abrogated by T-cell-dependent mechanisms, whereas major histocompatibility complex (MHC)-matched permissive A.BY/SnJ mice develop based on virus persistence. To define the role of T-cell-priming dendritic cells (DCs) in outcome myocarditis, DCs were analyzed this animal model course infection. both mouse strains, found be infectible with CVB3;...
There is increasing evidence that 2009 pandemic H1N1 influenza viruses have evolved after onset giving rise to severe epidemics in subsequent waves. However, it still remains unclear which viral determinants might contributed disease severity initiation. Here, we show distinct mutations the virus genome occurred with increased frequency declaration. Among those, a mutation hemagglutinin was identified increases binding human-like α2,6-linked sialic acids. Moreover, these conferred...
Abstract Purpose: To elicit a long-lasting antitumor immune response, CD8+ and CD4+ T cells should be activated. We attempted to isolate HLA-DR–presented peptides directly from dissected solid tumors, in particular renal cell carcinoma, identify MHC class II ligands tumor-associated antigens (TAA) for their use peptide-based immunotherapy. Experimental Design: Tumor specimens were analyzed by immunohistochemical staining HLA expression. subsequently isolated identified mass spectrometry....