A5079180261- RNA Research and Splicing
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- Cancer-related molecular mechanisms research
- Sarcoma Diagnosis and Treatment
- RNA and protein synthesis mechanisms
- Cancer, Hypoxia, and Metabolism
- CRISPR and Genetic Engineering
- Protein Degradation and Inhibitors
- Fungal and yeast genetics research
- Genomics and Chromatin Dynamics
- MicroRNA in disease regulation
- Cancer-related gene regulation
- Korean Peninsula Historical and Political Studies
- Spanish Literature and Culture Studies
- Cancer, Lipids, and Metabolism
- Early Modern Spanish Literature
- Epigenetics and DNA Methylation
- Peptidase Inhibition and Analysis
- interferon and immune responses
- Spanish Linguistics and Language Studies
- Medieval Iberian Studies
Stanford University
2023-2024
Nationwide Children's Hospital
2018-2024
The Ohio State University
2017-2022
The Ohio State University Wexner Medical Center
2019
University of Chile
2014-2017
Abstract Rhabdomyosarcoma (RMS) is an aggressive pediatric tumor with a poor prognosis for metastasis and recurrent disease. Large-scale sequencing endeavors demonstrate that Rhabdomyosarcomas have dearth of precisely targetable driver mutations. However, IGF-2 signaling known to be grossly altered in RMS. The insulin receptor (IR) exists two alternatively spliced isoforms, IR-A IR-B. molecule binds both its innate IGF-1 as well the variant A ( ) high affinity. Mitogenic proliferative via...
MDM2 is an oncogene and critical negative regulator of tumor suppressor p53. Genotoxic stress causes alternative splicing transcripts, which leads to alterations in p53 activity contributes tumorigenesis. MDM2-ALT1 one the alternatively spliced transcripts predominantly produced response genotoxic stress, comprised terminal coding exons 3 12. Previously, we found that SRSF1 induces by promoting exon 11 skipping. Here report SRSF2 antagonizes regulation facilitating inclusion through binding...
In Schizosaccharomyces pombe, ribosomal protein gene (RPG) promoters contain a TATA analogue element called the HomolD box. The HomolD-binding Rrn7 forms complex with RNA polymerase II machinery. Despite importance of ribosome biogenesis to cell survival, mechanisms involved in regulation transcription eukaryotic RPGs are unknown. this study, we identified as new substrate pleiotropic casein kinase 2 (CK2), which is regulator basal transcription. Recombinant from S. often used model organism...
In Schizosaccharomyces pombe , ribosomal protein gene ( RPG ) promoters contain a TATA box analog, the HomolD box, which is bound by Rrn7 protein. Despite importance of ribosome biogenesis for cell survival, mechanisms underlying transcription remain unknown. this study, we found that components RNA polymerase II RNAPII system, consisting initiation or general factors GTF s) TFIIA IIB IIE TATA‐binding TBP and holoenzyme, interacted directly with in vitro were able to form preinitiation...
Abstract Retroperitoneal liposarcoma (RPLPS) is one of the most common histologic subtypes soft tissue sarcoma (STS). Complete surgical resection remains mainstay treatment while high rate locoregional recurrence constitutes predominant cause mortality. Well- differentiated (WDLPS) and dedifferentiated (DDLPS) are frequent RPLPS present amplified MDM2 gene as a hallmark. However, there few reports evaluating role alternatively spliced transcripts in RPLPS. In this study, we assessed...
Patients with osteosarcoma (OS), a debilitating pediatric bone malignancy, have limited treatment options to combat aggressive disease. OS thrives on insulin growth factor (IGF)-mediated signaling that can facilitate cell proliferation. Previous efforts target IGF-1R were mostly unsuccessful, likely due compensatory through alternative splicing of the receptor (
Retroperitoneal liposarcoma (RPLPS) is one of the most common histologic subtypes soft tissue sarcoma (STS). Complete surgical resection remains mainstay treatment, while high rate locoregional recurrence constitutes predominant cause mortality. Well-differentiated (WDLPS) and dedifferentiated (DDLPS) are frequent RPLPS present amplified MDM2 gene as a hallmark. However, there few reports evaluating role alternatively spliced transcripts in RPLPS. In this study, we assessed MDM2-ALT2...
Abstract Alternative splicing is a process contributing to structural transcript variation and proteome diversity but often disrupted in cancer. Like most other transcripts, the insulin receptor (IN-R) undergoes alternative produce two isoforms: full-length IN-RB exon 11 skipped IN-RA isoform. The expression of these isoforms tightly regulated during development, however there an aberrant increase childhood cancers like Rhabdomyo- & Osteo-sarcoma. This increased deleterious cells because...
Abstract Alternative splicing of the MDM2 is an important means by which p53 upregulated to combat deleterious effects genotoxic stress. One splice variant, MDM2-ALT1, activated in response stress and comprised only two terminal coding exons 3 12 therefore lacks a binding domain. This variant has been identified number human tumors, including invasive carcinoma breast lung, as well soft tissue sarcomas. Despite its pervasiveness tumors therapeutic possibilities it presents, there very little...
<p>3D rendering of 60X confocal images taken SRSF2 (green) and SRRM2 (red) coimmunofluorescence in HeLa S3 cells after 12 hours UVC treatment. Colocalization channel is indicated by magenta color, while the blue color represents nucleus stained with DAPI.</p>
<p>3D rendering of 60X confocal images taken SRSF2 (green) and SRRM2 (red) coimmunofluorescence in HeLa S3 cells after 0 hours UVC treatment. Colocalization channel is indicated by magenta color, while the blue color represents nucleus stained with DAPI.</p>
<p>Supplemental Figure S1: Mutation of predicted binding sites for SRSF2 disrupt alternative splicing regulation the MDM2 3-11-12s minigene in response to cisplatinum. Supplemental S2: protein expression is increased after UVC treatment. S3: relocalized upon S4: has less affinity CCNL1 exon 4 under S5: Exogenous expressed nucleus. Table Oligonucleotides</p>
<p>3D rendering of 60X confocal images taken SRSF2 (green) and SRRM2 (red) coimmunofluorescence in HeLa S3 cells after 12 hours UVC treatment. Colocalization channel is indicated by magenta color, while the blue color represents nucleus stained with DAPI.</p>
<p>3D rendering of 60X confocal images taken SRSF2 (green) and SRRM2 (red) coimmunofluorescence in HeLa S3 cells after 0 hours UVC treatment. Colocalization channel is indicated by magenta color, while the blue color represents nucleus stained with DAPI.</p>
<div>Abstract<p><i>MDM2</i> is an oncogene and critical negative regulator of tumor suppressor p53. Genotoxic stress causes alternative splicing <i>MDM2</i> transcripts, which leads to alterations in p53 activity contributes tumorigenesis. <i>MDM2-ALT1</i> one the alternatively spliced transcripts predominantly produced response genotoxic stress, comprised terminal coding exons 3 12. Previously, we found that SRSF1 induces by promoting exon 11...
<div>Abstract<p><i>MDM2</i> is an oncogene and critical negative regulator of tumor suppressor p53. Genotoxic stress causes alternative splicing <i>MDM2</i> transcripts, which leads to alterations in p53 activity contributes tumorigenesis. <i>MDM2-ALT1</i> one the alternatively spliced transcripts predominantly produced response genotoxic stress, comprised terminal coding exons 3 12. Previously, we found that SRSF1 induces by promoting exon 11...
<p>Supplemental Figure S1: Mutation of predicted binding sites for SRSF2 disrupt alternative splicing regulation the MDM2 3-11-12s minigene in response to cisplatinum. Supplemental S2: protein expression is increased after UVC treatment. S3: relocalized upon S4: has less affinity CCNL1 exon 4 under S5: Exogenous expressed nucleus. Table Oligonucleotides</p>
Abstract MDM2 is an important negative regulator of protein stability and transcriptional activity tumor suppressor p53. We have previously shown that the alternatively spliced variant MDM2, MDM2-ALT1, induced specifically after exposure to ultraviolet light (UVC) cisplatin treatment associated with various soft tissue sarcomas, including rhabdomyosarcoma (RMS). also MDM2-ALT1 interferes normal heterodimer formation between MDM4 required for efficient p53 degradation by MDM2-mediated...
Alternative splicing of the MDM2 is an important means by which p53 upregulated to combat deleterious effects genotoxic stress. One splice variant, MDM2-ALT1, activated in response stress and comprised only two terminal coding exons 3 12 therefore lacks a binding domain. This variant has been identified number human tumors, including invasive carcinoma breast lung, as well soft tissue sarcomas. Despite its pervasiveness tumors therapeutic possibilities it presents, there very little known...
ABSTRACT MDM2 is an oncogene and critical negative regulator of tumor suppressor p53. Genotoxic stress causes alternative splicing transcripts, which leads to alterations in p53 activity contributes tumorigenesis. MDM2-ALT1 one transcripts predominantly produced response genotoxic comprised terminal coding exons 3 12. Previously, we found that SRSF1 induces by promoting exon 11 skipping. Here report SRSF2 antagonizes the regulation facilitating inclusion through binding at two conserved...