A5066546837- Herpesvirus Infections and Treatments
- interferon and immune responses
- MicroRNA in disease regulation
- Animal Virus Infections Studies
- Circular RNAs in diseases
- RNA and protein synthesis mechanisms
- Cytomegalovirus and herpesvirus research
- Monoclonal and Polyclonal Antibodies Research
- RNA regulation and disease
- Toxin Mechanisms and Immunotoxins
- Extracellular vesicles in disease
- Poxvirus research and outbreaks
- Glycosylation and Glycoproteins Research
- Viral gastroenteritis research and epidemiology
- Hepatitis C virus research
- Advanced Biosensing Techniques and Applications
- Genetics, Bioinformatics, and Biomedical Research
- Virus-based gene therapy research
- Cancer-related molecular mechanisms research
- Retinoids in leukemia and cellular processes
- RNA Research and Splicing
- Toxoplasma gondii Research Studies
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
University of Lincoln
2024
MRC University of Glasgow Centre for Virus Research
2014-2024
The Pirbright Institute
2017
University of Nottingham
2010-2013
University of Glasgow
2008-2010
Molecular recognition reagents are key tools for understanding biological processes and used universally by scientists to study protein expression, localisation interactions. Antibodies remain the most widely of such many show excellent performance, although some poorly characterised or have stability batch variability issues, supporting use alternative binding proteins as complementary applications. Here we report on Affimer research reagents. We selected 12 diverse molecular targets...
In animals, microRNAs (miRNAs) generally repress gene expression by binding to sites in the 3′-untranslated region (UTR) of target mRNAs. miRNAs have also been reported or activate 5′-UTR sites, but extent such regulation and factors that govern these different responses are unknown. Liver-specific miR-122 binds hepatitis C virus (HCV) RNA positively regulates viral life cycle, part stimulating HCV translation. Here, we characterize features allow translation via 5′-UTR. We find this is a...
Detection of viral nucleic acids plays a critical role in the induction intracellular host immune defences. However, temporal recruitment regulators to infecting genomes remains poorly defined due technical difficulties associated with low genome copy-number detection. Here we utilize 5-Ethynyl-2'-deoxyuridine (EdU) labelling herpes simplex virus 1 (HSV-1) DNA combination click chemistry examine sequential under multiplicity infection conditions. Following entry into nucleus, PML-nuclear...
ABSTRACT Studies with herpes simplex virus type 1 (HSV-1) have shown that secondary envelopment and release are blocked in mutants deleted for the tegument protein gene UL36 or UL37, leading to accumulation of DNA-containing capsids cytoplasm infected cells. The failure assemble infectious virions has meant roles these genes initial stages infection could not be investigated. To circumvent this, cells at a low multiplicity were fused form syncytia, thereby allowing released from nuclei...
Host innate immune defences play a critical role in restricting the intracellular propagation and pathogenesis of invading viral pathogens. Here we show that histone H3.3 chaperone HIRA (histone cell cycle regulator) associates with promyelocytic leukaemia nuclear bodies (PML-NBs) to stimulate induction against herpes simplex virus 1 (HSV-1) infection. Following activation signalling, localized at PML-NBs Janus-Associated Kinase (JAK), Cyclin Dependent (CDK), Sp100-dependent manner. RNA-seq...
ABSTRACT Herpesviruses have a characteristic particle structure comprising an icosahedral capsid, which contains the DNA genome and is, in turn, surrounded by proteinaceous tegument layer lipid envelope. In herpes simplex virus, interaction between capsid is limited to vertices involves two minor proteins, pUL17 pUL25, large inner protein pUL36. pUL25 form heterodimeric structure, vertex-specific component (CVSC), that lies on top of peripentonal triplexes, while pUL36 has been reported...
Secondary envelopment of herpes simplex virus type 1 has been demonstrated as taking place at the trans-Golgi network (TGN). The inner tegument proteins pUL36 and pUL37 envelope glycoproteins gD gE are known to be important for secondary envelopment. We compared cellular localizations capsids from a mutant lacking UL37 gene with those genes encoding gE. Although wild-type accumulated TGN, pUL37(-) were distributed throughout cytoplasm showed no association TGN-derived vesicles. This was in...
Abstract A 2022 canine gastroenteritis outbreak in the United Kingdom was associated with circulation of a new enteric coronavirus closely related to 2020 variant an additional spike gene recombination. The variants are unrelated coronavirus–like viruses human disease but represent model for population adaptation.
The P body protein LSm1 stimulates translation and replication of hepatitis C virus (HCV). As the liver-specific microRNA-122 (miR-122) is required for HCV associated with bodies, we investigated whether regulation by involves miR-122. Here, demonstrate that contributes to activation internal ribosome entry site (IRES)-driven This role specialized miR-122 activation, as depletion does not affect repressive function at 3′ untranslated region (UTR) sites, or miR-122–mediated cleavage a...
Abstract Herpesviruses are ubiquitous pathogens that cause a wide range of disease. Upon nuclear entry, their genomes associate with histones and chromatin modifying enzymes regulate the progression viral transcription outcome infection. While composition modification has been extensively studied on bulk populations infected cells by immunoprecipitation, this key regulatory process remains poorly defined at single-genome resolution. Here we use high-resolution quantitative imaging to...
Auteur(s) : A Roberts, FJ Rixon, D Pasdeloup MRC Virology Unit, Church Street, Glasgow G11 5JR, Ecosse Les Herpesvirus sont des virus enveloppes a ADN de grande taille la composition complexe. Ainsi, particule virale est composee trois structures majeures [1] : capside contenant le genome, membrane les glycoproteines d’enveloppe et, entre et membrane, tegument, une couche proteines amorphe dont structure fonction restent floues. La [...]