A5010419409- Vibrio bacteria research studies
- Aquaculture disease management and microbiota
- Bacterial biofilms and quorum sensing
- Yersinia bacterium, plague, ectoparasites research
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Gut microbiota and health
- Cystic Fibrosis Research Advances
- Antifungal resistance and susceptibility
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Protein Structure and Dynamics
- Sports and Physical Education Research
- Irish and British Studies
- Microbial Metabolic Engineering and Bioproduction
- CRISPR and Genetic Engineering
- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Political and Economic history of UK and US
- Salmonella and Campylobacter epidemiology
University of Washington
2022-2025
Tufts University
2016-2022
Boston University
2020
Harvey Mudd College
2016-2017
The alternative sigma factor RpoS is a central regulator of many stress responses in Escherichia coli level functional differs depending on the stress. effect these differing concentrations global transcriptional remains unclear. We investigated concentration transcriptome during stationary phase rich media. found that 23% genes E. genome are regulated by RpoS, and we identified RpoS-transcribed promoters. observed three distinct classes response to regulon: whose expression changes linearly...
Abstract DNA–protein interactions are central to fundamental cellular processes, yet widely implemented technologies for measuring these on a genome scale in bacteria laborious and capture only snapshot of binding events. We devised facile method mapping interaction sites vivo using the double-stranded DNA-specific cytosine deaminase toxin DddA. In 3D-seq (DddA-sequencing), strains containing DddA fused DNA-binding protein interest accumulate characteristic mutations DNA sequence adjacent...
Staphylococcus aureus is one of the most common pathogens isolated from lungs people with cystic fibrosis (CF), but little known about its ability to colonize this niche. We performed a transposon-sequencing (Tn-seq) screen identify genes necessary for S. growth in media prepared ex vivo CF sputum. identified 19 that were required all sputum tested and dozens more at least medium. Depleted mutants interest included insertions many important surviving metal starvation, as well primary...
Abstract The bacterial predator Bdellovibrio bacteriovorus is evolved to attack and kill other bacteria, including the human intestinal pathogen Vibrio cholerae . Although B exhibit a broad prey range, little known about genetic determinants of resistance sensitivity. Here we perform screen on V. identify five pathways contributing predation susceptibility. We find that essential virulence regulators ToxR/S increase susceptibility predation, as mutants these genes are more resistant...
is one of the most common pathogens isolated from lungs people with cystic fibrosis (CF), but little known about its ability to colonize this niche. We performed a Tn-seq screen identify genes necessary for
Within deep tissue sites, extracellular bacterial pathogens often replicate in clusters that are surrounded by immune cells. Disease is modulated interbacterial interactions as well bacterial-host cell resulting microbial growth, phagocytic attack and secretion of host antimicrobial factors. To overcome the limited ability to manipulate these infection we established a system for Yersinia pseudotuberculosis (Yptb) growth microfluidics-driven microdroplets regenerates social behavior tissues....
Abstract The alternative sigma factor RpoS is a central regulator of many stress responses in Escherichia coli. level functional differs depending on the stress. effect these differing concentrations global transcriptional remains unclear. We investigated concentration transcriptome during stationary phase rich media. show that 23% genes E. coli genome are regulated by level, and we identify RpoS-transcribed promoters. observe three distinct classes response to regulon: whose expression...
Abstract Within deep tissue sites, extracellular bacterial pathogens often replicate in clusters that are surrounded by immune cells. Disease is modulated interbacterial interactions as well bacterial-host cell resulting microbial growth, phagocytic attack and secretion of host antimicrobial factors. To overcome the limited ability to manipulate these infection we established a system for Yersinia pseudotuberculosis ( Yptb) growth microfluidics-driven microdroplets regenerates social...
Vibrio cholerae is a facultative pathogen that found naturally in aquatic environments around the world. Upon ingestion of contaminated water or food, V. can colonize human small intestine and induce profuse secretory diarrhea known as rice stool (RWS). During course infection undergoes through various transcriptional changes to adapt shifting environment. In early stage infection, bacteria rapidly replicate increase expression key virulence factors. The middle characterized by upregulation...
Abstract The vieSAB operon regulates motility, biofilm formation and cholera toxin production in the classical biotype of V. cholerae . Classical mutants lacking cyclic-di-GMP phosphodiesterase response regulator vieA or its cognate sensor kinase vieS fail to effectively colonize infant mouse small intestine, are less motile than wild-type strain, hyper-biofilm formers. However, deletion currently-circulating El Tor does not have a demonstrable effect on these phenotypes. To begin understand...