A5101555790- Histone Deacetylase Inhibitors Research
- Protein Degradation and Inhibitors
- Neuroinflammation and Neurodegeneration Mechanisms
- Epigenetics and DNA Methylation
- Immune cells in cancer
- Peptidase Inhibition and Analysis
- CRISPR and Genetic Engineering
- Antibiotic Resistance in Bacteria
- Kruppel-like factors research
- Oral microbiology and periodontitis research
- S100 Proteins and Annexins
- Cell Adhesion Molecules Research
- Health Systems, Economic Evaluations, Quality of Life
- Vibrio bacteria research studies
- Cancer-related gene regulation
- Neurotransmitter Receptor Influence on Behavior
- Neurogenesis and neuroplasticity mechanisms
- Neuropeptides and Animal Physiology
- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Barrier Structure and Function Studies
- Pharmaceutical Economics and Policy
- Cancer Immunotherapy and Biomarkers
- RNA and protein synthesis mechanisms
- Genetic Syndromes and Imprinting
Yale University
2024-2025
University of Utah
2024
University of British Columbia
2020-2024
Vancouver Coastal Health
2022-2023
Stanford University
2023
Stanford Medicine
2023
University of Minnesota System
2023
University of Washington
2021-2022
Carnegie Mellon University
2012-2022
University of Minnesota
2017-2022
Histone deacetylases (HDACs) are involved in diverse cellular regulatory mechanisms including non-canonical functions outside the chromatin environment. Several publications have demonstrated that selective HDAC inhibitors (HDACi) can influence tumor immunogenicity and functional activity of specific immune cells. In particular, inhibition HDAC6 has been reported to decrease growth several malignancies. However, there is still no clarity about components mediating this effect. study, we...
Large-language models have recently demonstrated impressive zero-shot capabilities in a variety of natural language tasks such as summarization, dialogue generation, and question-answering. Despite many promising applications clinical medicine, adoption these real-world settings has been largely limited by their tendency to generate incorrect sometimes even toxic statements. In this study, we develop Almanac, large model framework augmented with retrieval for medical guideline treatment...
As the primary innate immune cells of brain, microglia respond to damage and disease through pro-inflammatory release cytokines neuroinflammatory molecules. Histone acetylation is an activating transcriptional mark that regulates inflammatory gene expression. Inhibition histone deacetylase 3 (Hdac3) has been utilized in pre-clinical models depression, stroke, spinal cord injury improve recovery following injury, but molecular mechanisms underlying Hdac3's regulation expression not well...
As rapid responders to their environments, microglia engage in functions that are mirrored by cellular morphology. Microglia classically thought exhibit a ramified morphology under homeostatic conditions which switches an ameboid form during inflammatory conditions. However, display wide spectrum of morphologies outside this dichotomy, including rod-like, ramified, ameboid, and hypertrophic states, have been observed across brain regions, neurodevelopmental timepoints, various pathological...
Neuroinflammation is involved in the pathogenesis of almost every central nervous system disorder. As brain's innate immune cells, microglia fine tune their activity to a dynamic brain environment. Previous studies have shown that repeated bouts peripheral inflammation can trigger long-term changes microglial gene expression and function, form memory.
Abstract DNA–protein interactions are central to fundamental cellular processes, yet widely implemented technologies for measuring these on a genome scale in bacteria laborious and capture only snapshot of binding events. We devised facile method mapping interaction sites vivo using the double-stranded DNA-specific cytosine deaminase toxin DddA. In 3D-seq (DddA-sequencing), strains containing DddA fused DNA-binding protein interest accumulate characteristic mutations DNA sequence adjacent...
Introduction: The immune response within the central nervous system (CNS) is heightened after hemorrhagic stroke, yet association between inflammation and outcome unclear. Analysis of cerebrospinal fluid (CSF) from patients with external ventricular drains (EVDs) enables analysis CNS inflammatory profile. We hypothesize that stroke a more proinflammatory CSF profile will have worse outcomes at discharge. Methods: Using cytometric bead array, we measured panel cytokines in blood samples...
Bacterial survival is fraught with antagonism, including that deriving from viruses and competing bacterial cells. It now appreciated bacteria mount complex antiviral responses; however, whether a coordinated defense against threats undertaken not well understood. Previously, we showed Pseudomonas aeruginosa possess danger-sensing pathway critical fitness determinant during competition other bacteria. Here, conducted genome-wide screens in P. reveal three conserved widespread interbacterial...
Abstract Background Neuroinflammation is involved in the pathogenesis of almost every central nervous system disorder. As brain’s innate immune cells, microglia fine tune their activity to a dynamic brain environment. Previous studies have shown that repeated bouts peripheral inflammation can trigger long-term changes microglial gene expression and function, form memory. Methods Results In this study, we used multiple low-dose lipopolysaccharide (LPS) injections adult mice study acute...
Abstract As rapid responders to their environments, microglia engage in functions that are mirrored by cellular morphology. Microglia classically thought exhibit a ramified morphology under homeostatic conditions which switches an ameboid form during inflammatory conditions. However, display wide spectrum of morphologies outside this dichotomy, including rod-like, ramified, ameboid, and hypertrophic states, have been observed across brain regions, neurodevelopmental timepoints, various...
With rapidly expanding data collections becoming increasingly available, the application of Semantic Computing has become imperative to leverage this resource for industrial applications. This paper presents a survey in area Business Intelligence. We examine semantic analytical techniques and tools as applied prediction analysis decision support. also define role context Data Mining, Text Mining Big Analytics. Additionally, we describe how business is queried with Structured Natural Language...
Streptococcus mutans, a primary dental pathogen, has remarkable capacity to scavenge nutrients from the oral biofilm for its survival. Cystine is an amino acid dimer formed by oxidation of two cysteine residues that required optimal growth S. which modulates l-cystine uptake via recently identified transporters designated TcyABC and TcyDEFGH, have not been fully characterized. Using nonpolar tcyABC-deficient mutant (SmTcyABC), here, we report drastically diminished in mutant, whereas ability...
Abstract The brain’s extracellular matrix (ECM) regulates neuronal plasticity and animal behavior. ECM staining shows an aggregated pattern in a net-like structure around subset of neurons diffuse the interstitial matrix. However, understanding structural features deposition across various types subcellular compartments remains limited. To visualize organization assembly process hyaluronan-scaffolded brain, we fused HaloTag to HAPLN1, which links hyaluronan proteoglycans. Expression or...
Abstract Background Robust methods for the segmentation and analysis of cells in 3D time sequences (3D+t) are critical quantitative cell biology. While many automated perform very well, few generalize reliably to diverse datasets. Such could significantly benefit from at least minimal user guidance. Identification correction errors time-series data is prime importance proper validation subsequent analysis. The primary contribution this work a novel method interactive microscopy data, which...
Sall4 encodes a transcription factor and is known to participate in the pluripotency network of embryonic stem cells. expression be high early stage post-implantation mouse embryos. During post-gastrulation stages, highly expressed tail bud distal limb buds, where progenitor cells are maintained an undifferentiated status. The rapidly downregulated during development. We previously demonstrated that required for posterior axial skeleton development by conditional deletion T ( Brachyury )...
A main impediment to effective development of new therapeutics for central nervous system disorders, and the in vivo testing biological hypotheses brain, is ability rapidly measure effect novel agents treatment combinations on pathophysiology native brain tissue. We have developed a miniaturized implantable microdevice (IMD) platform, optimized direct stereotactic insertion into which enables simultaneous measurement multiple drug effects tissue situ. The IMD contains individual reservoirs...