A5022925847- Extracellular vesicles in disease
- Sperm and Testicular Function
- RNA Interference and Gene Delivery
- Spectroscopy Techniques in Biomedical and Chemical Research
- Viral Infections and Vectors
- Reproductive Physiology in Livestock
- Rabbits: Nutrition, Reproduction, Health
- Genetic and phenotypic traits in livestock
- Gold and Silver Nanoparticles Synthesis and Applications
- Reproductive System and Pregnancy
- Animal Nutrition and Physiology
- MicroRNA in disease regulation
- Urologic and reproductive health conditions
- Effects of Environmental Stressors on Livestock
- Reproductive Biology and Fertility
Mississippi State University
2022-2025
Sperm heterogeneity creates challenges for successful artificial insemination. Seminal plasma (SP) surrounding sperm is an excellent source detecting reliable non-invasive biomarkers of quality. Here, we isolated microRNAs (miRNAs) from SP-derived extracellular vesicles (SP-EV) boars with divergent quality statuses. Raw semen sexually mature was collected eight weeks. motility and normal morphology were analyzed, the classified as poor- or good-quality based on standard cutoffs 70%...
Artificial insemination (AI) plays a critical role in livestock reproduction, with semen quality being essential. In swine, AI primarily uses cool-stored adhering to industry standards assessed through routine analysis, yet fertility inconsistencies highlight the need for enhanced evaluation. Over 10-day storage at 17 °C, boar samples were analyzed motility, morphology, sperm membrane integrity, apoptosis, and oxidative stress indicators. Additionally, machine learning tools employed explore...
Ovarian follicular fluid (FF) is a dynamic environment that changes with the seasons, affecting follicle development, ovulation, and oocyte quality. Cells in follicles release tiny particles called extracellular vesicles (EVs) containing vital regulatory molecules, such as microRNAs (miRNAs). These miRNAs are pivotal facilitating communication within through diverse signaling information transfer forms. EV-coupled miRNA implicated to be associated ovarian function, growth response various...
Abstract Artificial insemination (AI) is the most important assisted reproductive technique devised to enhance genetic potential of animals by enabling widespread utilization elite males. In swine industry, AI with extended semen highly effective, several extenders developed for long-term storage. Regardless, gradual decline sperm quality during storage remains a major concern rapid dissemination. We hypothesized that existing sire-to-sire variations could reflect on survival prolonged...
Abstract Numerous studies have reported the negative effect of cottonseed gossypol on ovarian reserve follicles, impairing female fertility. Injuries resulting in depletion primordial follicles can lead to permanent infertility. At birth, mammals possess a fixed number which develop into primary, secondary, and antral continuous irreversible process. In this study, we evaluated potential effects dietary combination with parasitic infection follicular meat goats. A total 40 Boer x Spanish...
Sperm quality is essential to guarantee the success of assisted reproduction. However, selecting high-quality sperm and maintaining it during (cryo)preservation for high efficiency remains challenging in livestock A comprehensive understanding biology allows better assessment quality, which could replace conventional analyses used today predict fertility with low accuracy. Omics approaches have revealed numerous biomarkers associated various phenotypic traits such as survival storage,...
Abstract The pig industry relies heavily on artificial insemination as the primary breeding technique, using high-quality semen doses for genetic improvement. Semen consists of seminal plasma, a complex fluid that provides an optimal environment sperm maturation, viability, and fertilization. Seminal plasma contains membranous structures called extracellular vesicles (EVs), facilitating intercellular communication. These plasma-EVs (SP-EVs) contain microRNAs, which hold promise predictive...
Abstract Artificial insemination (AI) appears as a core of reproductive management in the hog industry. AI involves use chilled semen doses meeting routine thresholds for motility (≥ 70%) and morphology 80%) at collection. However, these standards exhibit gradual decline sperm quality, with likely divergent profiles during preservation that impact fertility outcomes. Hence, this study aims to unravel quality dynamics boar 7-d storage 17ºC. Semen (n = 25) from proven-fertile Duroc boars were...
Abstract Artificial insemination (AI) with fresh semen is the primary breeding tool in swine industry. However, heterogenous doses create challenges boar studs due to lack of consistent assessment criteria. Seminal plasma (SP) surrounding spermatozoa a great source reliable non-invasive biomarkers sperm quality. It contains wide array bioactive molecules, whose dynamic compositions response extrinsic and intrinsic factors influence motility morphology. Therefore, we hypothesize that miRNA...
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife