A5072471710- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Reproductive Physiology in Livestock
- Ovarian function and disorders
- Genetic and phenotypic traits in livestock
- Molecular Biology Techniques and Applications
- Pregnancy-related medical research
- Reproductive biology and impacts on aquatic species
- Quantum Dots Synthesis And Properties
- Extracellular vesicles in disease
- Reproductive System and Pregnancy
- bioluminescence and chemiluminescence research
- Animal Diversity and Health Studies
- Muscle metabolism and nutrition
- Identification and Quantification in Food
- Botanical Research and Applications
- Animal Nutrition and Physiology
- Nanoparticles: synthesis and applications
- Effects of Environmental Stressors on Livestock
- GDF15 and Related Biomarkers
- Selenium in Biological Systems
- Birth, Development, and Health
- Tea Polyphenols and Effects
- Probiotics and Fermented Foods
Mississippi State University
2016-2025
Institut de Recherches Médicales et d’Etudes des Plantes Médicinales
2024
Division of Undergraduate Education
2021
Memphis Zoo
2013
University of Arizona
2005-2010
UConn Health
2010
Jackson Laboratory
2007
University of Wisconsin–Madison
2007
UCLouvain
2000-2005
Institut National de la Recherche Agronomique
2001
This study investigated the impact of an increased arginine (ARG) level in a boar diet on semen production, sperm quality, and seminal plasma proteome. Adult Nebraska Index Line boars were assigned to two groups, one receiving control with 0.77% (n = 4) other high-arginine 1.77% 5). Semen was collected twice week over whole experiment, including before, six weeks during, after supplementation. Parameters such as volume concentration assessed immediately collection, alongside motility...
Abstract Background Male infertility is a major problem for mammalian reproduction. However, molecular details including the underlying mechanisms of male fertility are still not known. A thorough understanding these essential obtaining consistently high reproductive efficiency and to ensure lower cost time-loss by breeder. Results Using low bull spermatozoa, here we employed differential detergent fractionation multidimensional protein identification technology (DDF-Mud PIT) identified 125...
Abstract Background Cancer chemoprevention is a new approach in cancer prevention, which chemical agents are used to prevent normal and/or high-risk populations. Although has shown promise some epithelial cancers, currently available preventive limited and the costly, generally with side effects. Natural products, such as grape seed, green tea, certain herbs have demonstrated anti-cancer To find natural product that can be of cancer, we tested Arizona cactus fruit solution, aqueous extracts...
Advances in nanotechnology have permitted molecular-based targeting of cells through safe and biocompatible magnetic nanoparticles (MNP). Their use to detect remove damaged spermatozoa from semen doses could be great interest. Here, MNP were synthesized tested for their ability target apoptotic (annexin V) acrosome-reacted (lectin) boar spermatozoa, high-throughout retrieval a field (nanoselection). The potential impacts nanoselection on sperm functions performance offspring sired by...
Sperm heterogeneity creates challenges for successful artificial insemination. Seminal plasma (SP) surrounding sperm is an excellent source detecting reliable non-invasive biomarkers of quality. Here, we isolated microRNAs (miRNAs) from SP-derived extracellular vesicles (SP-EV) boars with divergent quality statuses. Raw semen sexually mature was collected eight weeks. motility and normal morphology were analyzed, the classified as poor- or good-quality based on standard cutoffs 70%...
Abstract Background Fertility is one of the most critical factors controlling biological and financial performance animal production systems genetic improvement lines. The objective this study was to identify molecular defects in sperm that are responsible for uncompensable fertility Holstein bulls. We performed a comprehensive genome wide analysis single nucleotide polymorphisms (SNP) bull followed by second-stage replication additional bulls restricted set markers. Results In Phase I...
<h3>Objectives:</h3> Human papillomavirus (HPV) infection is closely associated with the development of more than 95% cervical cancer. Clinical trials using several chemopreventive agents are underway, but results inconclusive. Most used in inhibited growth cancer cells vitro, and about half patients had some degree clinical responses; however, therapeutic effect was confounded by high rates spontaneous regression relapse. The selection nontoxic especially food, beverage, natural products...
Various obstacles are encountered by mammalian spermatozoa during their journey through the female genital tract, and only few or none will reach site of fertilization. Currently, there limited technical approaches for non-invasive investigation migration after insemination. As knowledge surrounding sperm behavior throughout tract still remains elusive, recent development self-illuminating quantum dot nanoparticles may present a potential means real-time in vitro vivo monitoring...
L-arginine is considered to be one of the most versatile amino acids due fact that it serves as a precursor for many important molecules in cellular physiology. When supplemented diet, can increase number implantation sites mice and rats, suggesting an effect at level endometrium. To this end, study determined has on apoptosis cell proliferation human endometrial RL95-2 cells. physiological (200 micromol/L) supra-physiological (800 concentrations increased days 2 4 post-treatment with...
There is a clear need for low-cost, self-applied, long-lasting approaches to prevent human immunodeficiency virus (HIV) infection in both men and women, even with the advent of pre-exposure prophylaxis (PrEP). Broadly neutralizing antibodies represent an option improve HIV prophylaxis, but intravenous delivery, cold-chain stability requirements, low cervicovaginal concentrations, cost may preclude their use. Here, we present approach express anti-GP120 broadly antibody PGT121 primary site...
Contents The aim of this retrospective study was to compare the number follicles, cumulus oocyte complexes (COCs) and cultured In Vitro Produced (IVP) embryos obtained from 1396 non‐stimulated Ovum Pick‐up (OPU) sessions on 81 donor animals in a twice weekly OPU scheme. Results were 640 following FSH‐LH superstimulation, 112 donors subjected once every 2 weeks. stimulation protocol started with insertion an ear implant containing 3 mg norgestomet (Crestar, Intervet, Belgium) 8 days before...
The fertility performance of animals is still a mystery and the full comprehension mammalian gametes maturation early embryonic development remains to be elucidated. recent in nanotechnology offers new opportunity for real-time study reproductive cells their physiological environments. As first step toward that goal, we evaluated effectiveness fluorescent luminescent nanoparticle vitro ex vivo imaging porcine gametes. Freshly harvested boar sperm were labeled with red-shifted (655 nm)...
Temporal pattern of expression Cu/Zn and Mn superoxide dismutases (SODs) was investigated in bovine oocytes embryos produced vitro two different culture conditions vivo after superovulation. SODs were examined at a transcriptional level single by reverse transcriptase–polymerase chain reaction (RT-PCR) and, protein level, Western blotting on pools embryos. mRNA encoding SOD detected throughout preattachment development as well derived morulae blastocysts. Transcripts for gene most immature...
Nanoparticles have emerged as key materials for developing applications in nanomedicine, nanobiotechnology, bioimaging and theranostics. Existing technologies include bioluminescent resonance energy transfer-conjugated quantum dots (BRET-QDs). Despite the current use of BRET-QDs bioimaging, there are strong concerns about QD nanocomposites containing cadmium which exhibits potential cellular toxicity. In this study, composites comprised magnetic nanoparticles firefly luciferase (Photinus...
Abstract Innumerable similarities in reproductive cyclicity and hormonal alterations highlight the considerable utility of mare to study aspects follicular dynamics function view largely constricted, human research subjects. The bi-directional communication between growing oocyte surrounding somatic cells embodies hallmark mammalian development, partially mediated by extracellular vesicles (EVs) encapsulated with microRNAs (miRNAs) present fluid (FF). Here, we aimed decipher miRNAs EVs from...
Mature spermatozoa contain numerous epididymal and seminal plasma proteins, which full identification through high-throughput technologies may allow for a better understanding of the sperm biology. Therefore, we conducted global proteomic analysis boar shotgun gel-based methodologies.The total proteins were extracted from mature subjecsted to proteome analyses. Functional analyses gene ontology representations pathway enrichments on dataset, followed by immunology expression validations....
The protective effect of natural products such as fruits and vegetables against cancer has attracted great attention because their fewer side effects therefore, potentially greater safety. We have previously reported that cactus pear mixture aqueous extract (CME) reduces gynecologic cells growth by inducting apoptosis. This study aimed to elucidate the cellular pathway(s) triggered CME in cells. Normal, immortalized ovarian (OVCA420, SKOV3) were treated with 5 10% CME. After 2 days...