A5039400819- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Circular RNAs in diseases
- Single-cell and spatial transcriptomics
- Lymphatic System and Diseases
- Kruppel-like factors research
- MicroRNA in disease regulation
- IL-33, ST2, and ILC Pathways
- RNA Research and Splicing
- Cancer Cells and Metastasis
ETH Zurich
2020-2022
École Polytechnique Fédérale de Lausanne
2020-2021
University of Zurich
2020-2021
Board of the Swiss Federal Institutes of Technology
2021
Abstract Recent studies have revealed the importance of long noncoding RNAs (lncRNAs) as tissue-specific regulators gene expression. There is ample evidence that distinct types vasculature undergo tight transcriptional control to preserve their structure, identity, and functions. We determine a comprehensive map lineage-specific lncRNAs in human dermal lymphatic blood vascular endothelial cells (LECs BECs), combining RNA-Seq CAGE-Seq. Subsequent antisense oligonucleotide-knockdown...
The lymphatic system plays a crucial role in immunity and lymph nodes (LNs) undergo drastic remodeling during inflammation. Here, we used single-cell RNA sequencing to investigate transcriptional changes endothelial cells (LECs) LNs draining naïve inflamed skin. We found that subsets of LECs lining the different LN sinuses responded individually skin inflammation, suggesting they exert distinct functions under pathological conditions. Among genes dysregulated confirmed an up-regulation CD200...
Tumor-draining lymph nodes (LNs), composed of lymphocytes, antigen-presenting cells, and stromal are highly relevant for tumor immunity the efficacy immunotherapies. Lymphatic endothelial cells (LECs) represent an important cell type within LNs, several distinct subsets LECs that interact with various immune regulate responses have been identified. In this study, we used single-cell RNA sequencing (scRNA-seq) to characterize from LNs draining B16F10 melanomas compared non-tumor-draining LNs....
Abstract Recent studies have revealed the importance of long noncoding RNAs (lncRNAs) as tissue-specific regulators gene expression. There is ample evidence that distinct types vasculature undergo tight transcriptional control to preserve their structure, identity, and functions. We determined, for first time, global lineage-specific lncRNAome human dermal blood lymphatic endothelial cells (BECs LECs), combining RNA-Seq CAGE-Seq. A subsequent genome-wide antisense oligonucleotide-knockdown...
Abstract Background The lymphatic and the blood vasculature are closely related systems that collaborate to ensure organism’s physiological function. Despite their common developmental origin, they present distinct functional fates in adulthood rely on robust lineage-specific regulatory programs. recent technological boost sequencing approaches unveiled long noncoding RNAs (lncRNAs) as prominent players of various gene expression levels a cell-type-specific manner. Results To investigate...