A5086634980- Streptococcal Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Neonatal and Maternal Infections
- Infective Endocarditis Diagnosis and Management
- Atherosclerosis and Cardiovascular Diseases
- Cholesterol and Lipid Metabolism
- Photosynthetic Processes and Mechanisms
- Cancer, Lipids, and Metabolism
- Lipid metabolism and disorders
- Bacterial biofilms and quorum sensing
- Proteins in Food Systems
- Plant Reproductive Biology
- Photodynamic Therapy Research Studies
- Enzyme Production and Characterization
- Microbial Metabolic Engineering and Bioproduction
- RNA and protein synthesis mechanisms
- Lipoproteins and Cardiovascular Health
- Enzyme Structure and Function
- Bacterial Infections and Vaccines
- Bioactive Compounds and Antitumor Agents
- Clusterin in disease pathology
- Protease and Inhibitor Mechanisms
- Food Quality and Safety Studies
- Probiotics and Fermented Foods
- Lipid Membrane Structure and Behavior
Inner Mongolia Agricultural University
2007-2021
Shanxi Normal University
2020
Plasma (Russia)
2015
Ministry of Agriculture
2014
West Virginia University
2005-2009
University of Toledo
2005
The streptococcal collagen-like protein-1, Scl1, is widely expressed by the well-recognized human pathogen group A Streptococcus (GAS). Screening of ligands for binding to recombinant Scl1 identified cellular fibronectin and laminin as partners. Both interacted with globular domain which also able bind low-density lipoprotein. Native mediated GAS adherence ligand-coated glass cover slips promoted internalization into HEp-2 cells. This work identifies new protein that are known be important...
Summary Non‐specific activation of the complement system is regulated by plasma glycoprotein factor H (FH). Bacteria can avoid complement‐mediated opsonization and phagocytosis through acquiring FH to cell surface. Here, we characterize an interaction between streptococcal collagen‐like protein Scl1.6 M6‐type group A Streptococcus (GAS) FH. Using affinity chromatography with immobilized recombinant protein, co‐eluted human proteins molecular weight 155 kDa, 43 kDa 38 kDa. Mass spectrometry...
Summary Several bacterial genera express proteins that contain collagen‐like regions, which are associated with variable (V) non‐collagenous regions. The streptococcal proteins, Scl1 and Scl2, of group A Streptococcus (GAS) members this ‘prokaryotic collagen’ family, they too an amino‐terminal V region unknown function. Here, we use recombinant rScl constructs, derived from several Scl2 variants, affinity chromatography to identify Scl ligands present in human plasma. First, show Scl1, but...
ABSTRACT Plasma lipoproteins (VLDL, LDL, Lp[a] and HDL) function primarily in lipid transport among tissues organs. However, cumulative evidence suggests that may also prevent bacterial, viral parasitic infections are therefore a component of innate immunity. Lipoproteins can detoxify lipopolysaccharide lipoteichoic acid. Infections induce oxidation oxLDL turn plays important anti‐infective roles protects against endotoxin‐induced tissue damage. There is apo(a) protective pathogens. Taken...
Streptococcal collagen-like protein 1 (Scl1) is a virulence factor on the surface of group A Streptococcus (GAS). We have reported previously that several Scl1 proteins derived from various M-type GAS strains, including M41, can bind to low-density lipoprotein, but M6-type strain cannot. Here, we demonstrated recombinant protein, designated C176, Scl1.41 M41-type also binds both plasma and purified high-density lipoprotein (HDL). Next, determined intact noncollagenous region C176 was...
We have previously demonstrated that high-density lipoprotein (HDL) can specifically bind to streptococcal collagen-like protein 1 (Scl1) of M41-type group A Streptococcus (GAS). However, the pathological or physiological significance Scl1-HDL interaction is unknown. Here, hypothesis HDL acts as an opsonin enhance phagocytosis HDL-bound GAS by monocytes given some scavenger receptors mediate endocytosis was tested using FITC-labeled bacteria, human U937 and for phagocytic assays. (10 µg/mL)...
Low-density lipoprotein (LDL) binds to group A Streptococcus (GAS) through Sc11 protein, and scavenger receptor CD36 of monocyte mediates the endocytosis native or modified LDL. Therefore, we hypothesized that LDL might be an opsonin enhancing phagocytosis LDL-bound GAS by monocyte. The results showed could significantly promote U937 cell phagocytose M28 (ATCC BAA1064) M41 12373, AM41)-type GAS, rates were increased, compared with LDL-free group. LDL, however, did not enhance (CMCC 32198,...
Context: Hen egg low-density lipoprotein (heLDL), which is present in large quantities yolk, share a high identity with human apolipoprotein B-100 precursor.Objective: This study investigated the use of heLDL as macrophage-targeted drug delivery carrier against intracellular Staphylococcus aureus.Methods: Rifapentine (RPT) was incorporated into (RPT–heLDL). aureus ATCC 29740 and U937 macrophage were used infection models.Results conclusion: The loading efficiency RPT 66.10 ± 2.28 μg RPT/mg...
Abstract Plasma lipoproteins [VLDL, LDL, and HDL] function primarily in lipid transport among tissues organs. However, the cumulative evidence may support that as part of innate immunity, also broadly prevent or inhibit bacterial, viral parasitic infections. Lipoproteins can detoxify lipopolysaccharide lipoteichoic acid. neutralize a vast enveloped non-enveloped DNA RNA viruses. Staphylococcus aureus α-toxin is bound inactivated by purified LDL. LDL directly binds to some bacteria, but...
We recently showed that both nontypeable Haemophilus influenzae (NTHi) and its surface plasminogen- (Plg-) binding proteins interact with lipoprotein(a) (Lp(a)) in a lysine-dependent manner. Because Lp(a) can be taken up by macrophages, we postulated it serves as an opsonin to enhance phagocytosis of NTHi macrophages. Based on colony-forming unit (CFU) counts, was found increase U937 macrophage-mediated NTHi49247 NTHi49766 34% 43%, respectively, after 120 min. In contrast, did not...