A5075307797- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Biochemical and Molecular Research
- HIV Research and Treatment
- Immune Cell Function and Interaction
- Cancer-related gene regulation
- Digestive system and related health
- Cytomegalovirus and herpesvirus research
- Wnt/β-catenin signaling in development and cancer
- Bacterial Genetics and Biotechnology
- Chemical synthesis and alkaloids
- Antibiotic Use and Resistance
- T-cell and B-cell Immunology
- Neurotransmitter Receptor Influence on Behavior
- Infectious Diseases and Tuberculosis
- Psychedelics and Drug Studies
- Diagnosis and treatment of tuberculosis
- Antibiotics Pharmacokinetics and Efficacy
- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
Michigan State University
2012-2023
University of California, Santa Cruz
2021
Calvin University
2013
Van Andel Institute
2011-2012
Recent genome-wide association studies of individuals Asian and European descent have found that SNPs located within the genomic region (1p31.3) encoding Wntless (Wls)/Gpr177 protein are associated significantly with reduced bone mineral density. Wls / Gpr177 is a newly identified chaperone specifically escorts Wnt ligands for secretion. Given strong functional between signaling pathways development homeostasis, we generated osteoblast-specific Wls-deficient ( Ocn-Cre;Wls-flox ) mice....
Summary During pathogenesis, M ycobacterium tuberculosis ( Mtb ) colonizes environments, such as the macrophage or necrotic granuloma, that are acidic and rich in cholesterol fatty acids. The goal of this study was to examine how pH available carbon sources interact regulate physiology. Here we report growth at requires host‐associated function intersection glycolysis TCA cycle, pyruvate, acetate, oxaloacetate cholesterol. In contrast, other tested sources, fully arrests its establishes a...
Mycobacterium tuberculosis (Mtb) senses and adapts to acidic environments during the course of infection. Acidic pH-dependent adaptations include induction metabolic genes associated with anaplerosis growth arrest on specific carbon sources. Here we report that deletion isocitrate lyase or phosphoenolpyruvate carboxykinase results in reduced at pH altered metabolite profiles, supporting remodeling anaplerotic metabolism is required for adaptation. Mtb cultured 5.7 minimal medium containing...
Low-density lipoprotein receptor-related proteins 5 and 6 (Lrp5 Lrp6) are co-receptors of Wnt ligands play important roles in Wnt/β-catenin signal transduction. Mice homozygous for a germline deletion Lrp6 die at birth with several associated defects, while Lrp5-deficient mice viable. Here, we conditionally deleted Lrp5 and/or the mouse gut ((gut-/-)) by crossing carrying floxed alleles to strain expressing Cre recombinase from villin promoter (villin-Cre). The changes morphology,...
ABSTRACT Highly active antiretroviral therapy (HAART) is able to suppress human immunodeficiency virus type 1 (HIV-1) undetectable levels in the majority of patients, but eradication has not been achieved because latent viral reservoirs persist, particularly resting CD4 + T lymphocytes. It generally understood that HIV-1 does efficiently infect cells, and infection those cells may arise when infected lymphoblasts return state. In this study, we found stimulation by endothelial can render...
In defined media supplemented with single carbon sources, Mycobacterium tuberculosis (Mtb) exhibits source specific growth restriction. When supplied glycerol as the sole at pH 5.7, Mtb establishes a metabolically active state of nonreplicating persistence known acid arrest. We hypothesized that arrest on is not metabolic restriction, but rather an adaptive response. To test this hypothesis, we selected for and identified several mutants could grow under these restrictive conditions. All...
Abstract Background Vpr is exclusively expressed in primate lentiviruses and contributes to viral replication disease progression vivo. HIV-1 has two major activities vitro: arrest of cell cycle the G2 phase (G2 arrest), enhancement macrophages. Previously, we reported a potent restriction human CD4 + CEM.NKR (NKR) T cells, where wild-type (WT) was inhibited by almost 1,000-fold. From parental NKR isolated eight clones limiting dilution. These showed three levels resistance WT infection:...
Abstract Objective: Medical residents are an important group for antimicrobial stewardship programs (ASPs) to target with interventions aimed at improving antibiotic prescribing. In this study, we compared prescribing practices of 2 academic medical teams receiving different ASP training approaches along a hospitalist control group. Design: Retrospective cohort study comparing guideline-concordant 3 common infections among family medicine (FM) resident service, internal (IM) and...
Abstract In defined media supplemented with single carbon sources, Mycobacterium tuberculosis (Mtb) exhibits source specific growth restriction. When supplied glycerol as the sole at pH 5.7, Mtb establishes a metabolically active state of nonreplicating persistence known acid arrest. We hypothesized that acidic arrest on is not metabolic restriction, but rather an adaptive response. To test this hypothesis, we conducted forward genetic screens identified several mutants could grow under...
Abstract Mycobacterium tuberculosis (Mtb) establishes a state of non-replicating persistence when it is cultured at acidic pH with glycerol as sole carbon source. Growth can be restored by spontaneous mutations in the ppe51 gene or supplementation pyruvate, supporting that acid growth arrests genetically controlled, adaptive process and not simply physiological limitation associated pH. Transcriptional profiling identified methylcitrate synthase dehydratase genes ( prpC prpD , respectively)...
Mycobacteria can colonize environments where the availability of metal ions is limited. Biological or inorganic chelators play an important role in limiting availability, and we developed a model to examine Mycobacterium smegmatis survival presence chelator sodium citrate. We observed that instead restricting M. growth, concentrated citrate killed smegmatis. RNAseq analysis during treatment revealed transcriptional signatures starvation hyperosmotic stress. Notably, stress, individually, do...
Abstract Mycobacterium tuberculosis (Mtb) senses and adapts to acidic environments during the course of infection. Acidic pH-dependent adaptations include induction metabolic genes associated with anaplerosis growth arrest on specific carbon sources. In this study, reverse forward genetic studies were undertaken define new mechanisms underlying adaptations. Here we report that deletion isocitrate lyase ( icl1/2 ) or phosphoenolpyruvate carboxykinase pckA results in reduced at pH altered...