A5043916529- Nuclear Structure and Function
- RNA Research and Splicing
- Reproductive Biology and Fertility
- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- Reproductive Health and Technologies
- Cellular transport and secretion
- Protein Tyrosine Phosphatases
- Protein Kinase Regulation and GTPase Signaling
- Assisted Reproductive Technology and Twin Pregnancy
- Prenatal Screening and Diagnostics
- Epigenetics and DNA Methylation
- Birth, Development, and Health
- Skin and Cellular Biology Research
- Galectins and Cancer Biology
- Chromatin Remodeling and Cancer
- Renal and related cancers
- Lipid Membrane Structure and Behavior
- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- Glycosylation and Glycoproteins Research
- ATP Synthase and ATPases Research
- Genetic Neurodegenerative Diseases
- Enzyme Structure and Function
- Heat shock proteins research
European Molecular Biology Laboratory
2025
University of Amsterdam
2018-2023
Amsterdam University Medical Centers
2021-2023
University of Aveiro
2016-2019
National Institutes of Health
1980
The main form of intracellular protein glycosylation (O-GlcNAc) is reversible and has been mapped on thousands cytoplasmic nuclear proteins, including RNA polymerase II, transcription factors, chromatin modifiers. O-GlcNAc modification catalyzed by a single enzyme known as Transferase, that required for mammalian early development. Yet, neither the regulatory function O-GlcNAcylation in embryo nor embryonic proteome have documented. Here, we devised strategy to enzymatically remove from...
Lamina-associated polypeptide 1 (LAP1) is a type II transmembrane protein of the inner nuclear membrane encoded by human gene TOR1AIP1. LAP1 involved in maintaining envelope structure and appears be positioning lamins chromatin. To date, LAP1's precise function has not been fully elucidated but analysis its interacting proteins will permit unraveling putative associations to specific cellular pathways processes. By assessing public databases it was possible identify interactome, this...
Abstract Background Spermatogonial stem cell transplantation (SSCT) is proposed as a fertility therapy for childhood cancer survivors. SSCT starts with cryopreserving testicular biopsy prior to gonadotoxic treatments such treatments. When the survivor reaches adulthood and desires biological children, thawed SSCs are propagated in vitro subsequently auto-transplanted back into their testis. However, culturing stress during long-term propagation can result epigenetic changes SSCs, DNA...
Spermatogenesis comprises highly complex differentiation processes. Nuclear envelope (NE) proteins have been associated with these processes, including lamins, lamina-associated polypeptide (LAP) 2 and the lamin B-receptor. LAP1 is an important NE protein whose function has not fully elucidated, but several binding partners allow predicting putative functions. To date, had spermatogenesis. In this study, expression cellular/subcellular localization during spermatogenesis in human mouse...
In medicine, safety and efficacy are the two pillars on which implementation of novel treatments rest. To protect patient from unnecessary or unsafe treatments, usually, a stringent path (pre) clinical testing is followed before treatment introduced into routine care. However, in reproductive medicine several techniques have been clinically without elaborate preclinical studies. Moreover, may harbor risks not only for patients undergoing treatment, but also offspring conceived through these...
BRI2 is a ubiquitously expressed type II transmembrane phosphoprotein. undergoes proteolytic processing into secreted fragments and during the maturation process it suffers post-translational modifications. Of particular relevance, protein phosphatase 1 (PP1) interacting protein, where PP1 able to dephosphorylate former. Further, disruption of BRI2:PP1 complex, using binding motif mutants, leads increased phosphorylation levels. However, physiological function remains elusive; although...
Abstract TorsinA is a member of the AAA+ superfamily adenosine triphosphatases. These proteins have numerous biological functions, including vesicle fusion, cytoskeleton dynamics, intracellular trafficking, protein folding, and degradation as well organelle biogenesis. Of particular interest torsinA, which mainly located in endoplasmic reticulum (ER) nuclear envelope (NE). Interestingly, mutations TOR1A gene (the encoding torsinA) are associated with DYT1 dystonia preferential localization...
The paternal preconception environment has been implicated as a modulator of phenotypic traits and disease risk in F1 offspring. However, the prevalence mechanisms such intergenerational epigenetic inheritance (IEI) mammals remain poorly defined. Moreover, interplay between exposure, genetics, age on emergent offspring features is unexplored. Here, we measure quantitative impact three environments early embryogenesis across genetic backgrounds. Using vitro fertilisation (IVF) at scale,...
Abstract Among many deleterious ramifications of oncological treatments, there is permanent male infertility due to the damage spermatogonial stem cells (SSC) in testes after chemotherapy or irradiation. For those patients that cannot produce sperm before cancer treatment, because prepubertal age, are no clinical options available father a child. To preserve fertility childhood patients, freezing testis biopsy already offered while treatment using this still under development, including cell...