A5041145724- RNA Research and Splicing
- RNA modifications and cancer
- Renal and related cancers
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Birth, Development, and Health
- Genomics and Chromatin Dynamics
- Sexual Differentiation and Disorders
- Sperm and Testicular Function
- CRISPR and Genetic Engineering
- Developmental Biology and Gene Regulation
- Prenatal Screening and Diagnostics
Centre National de la Recherche Scientifique
2020-2022
Université Paris Sciences et Lettres
2020-2022
Inserm
2020-2022
Institut Curie
2020-2022
Institut de Biologie de l'École Normale Supérieure
2018
École Normale Supérieure - PSL
2018
Cis-regulation plays an essential role in the control of gene expression, and is particularly complex poorly understood for developmental genes, which are subject to multiple levels modulation. In this study, we performed a global analysis cis-acting elements involved zebrafish krox20. krox20 encodes transcription factor required hindbrain segmentation patterning, morphogenetic process highly conserved during vertebrate evolution. Chromatin accessibility reveals cis-regulatory landscape that...
Abstract Endogenous retroviruses (ERVs) are abundant and heterogenous groups of integrated retroviral sequences that impact genome regulation cell physiology throughout their RNA-centered life cycle 1 . Failure to repress ERVs is associated with cancer, infertility, senescence neurodegenerative diseases 2–4 Here, using an unbiased genome-scale CRISPR knockout screen in mouse embryonic stem cells, we identify m 6 A RNA methylation as a novel means ERV restriction. Methylation mRNAs catalyzed...
The paternal preconception environment has been implicated as a modulator of phenotypic traits and disease risk in F1 offspring. However, the prevalence mechanisms such intergenerational epigenetic inheritance (IEI) mammals remain poorly defined. Moreover, interplay between exposure, genetics, age on emergent offspring features is unexplored. Here, we measure quantitative impact three environments early embryogenesis across genetic backgrounds. Using vitro fertilisation (IVF) at scale,...
Abstract DNA methylation plays a critical role in spermatogenesis, as evidenced by the male sterility of methyltransferase (DNMT) mutant mice. Here, we report striking division labor establishment landscape germ cells and its functions spermatogenesis: while DNMT3C is essential for preventing retrotransposons from interfering with meiosis, DNMT3A broadly methylates genome—at exception DNMT3C-dependent retrotransposons—and controls spermatogonial stem cell (SSC) plasticity. By reconstructing...