Bisphenols may negatively impact human health. In this study, we propose the use of HPLC–FLD for simultaneous determination bisphenols in pericardial fluid samples collected from patients with coronary artery disease undergoing bypass surgery. For sample preparation, a fast, simple, and ”green” DLLME method was used, achieving mean recovery values range 62%–98% relative standard deviations between 2% 6% all analytes. Quantitative analysis then performed by LC–MS/MS on triple quadrupole (QqQ)...

A simple, inexpensive and robust high-performance liquid chromatography diode array detector (HPC-DAD) procedures are proposed to analyse food dyes in beverages, hard candy fish roe samples. An ether-linked phenyl stationary phase provides sufficient selectivity chromatographic performance for separation of 11 sulfonated azo dyes. Beverage samples were only diluted (and degassed when needed) before analysis. Solid-phase extraction (SPE) or matrix solid-phase dispersion (MSPD) efficient the...

Background: Identification and quantitative determination of analytes released from the packaging material is undoubtedly a difficult tricky task, requiring chemical analyst to develop an individual approach obtain reliable analytical information. Unfortunately, it still challenging for scientists determine bisphenols at trace or even ultra-trace levels in samples characterized by very complex, often variable, matrix composition. Objective: Optimization application QuEChERS/d-SPE coupled...

Plastic pollution, where bisphenol A (BPA) is widely used in its production, has gained popularity. BPA omnipresence and toxicity, especially for infants, led food safety authorities to place restrictions on usage. It the introduction of marked ‘BPA-free’-labelled products, often replaced by other bisphenols (BPs) which are suspected being similar or even more toxic than BPA. Moreover, free forms BPs dangerous their conjugated conjugation less effective infants adults. Considering that human...

Alkaloids have protective functions for plants and can play an important role in living organisms. may a wide range of pharmacological activities. Many them cytotoxic activity. Nowadays, cancer has become serious public health problem. Searching effective drugs with anticancer activity is one the most significant challenges modern scientific research. The aim this study was investigation extracts obtained from Corydalis lutea root herb, Dicentra spectabilis Fumaria officinalis, Macleaya...

An important strategy for treating neurodegenerative disorders is to maintain the levels of acetylcholine in synaptic cleft by blocking cholinesterases. Searching new effective compounds with inhibited acetylcholinesterase and butyrylcholinesterase activity one most significant challenges modern scientific research. The aim this study was optimization condition cholinesterase determination high-performance liquid chromatography coupled diode array detector (HPLC-DAD) terms concentrations...

The application of RP-HPLC with a diode array detector for identification and quantitative analysis pesticides in sunflower seed samples is demonstrated. An HPLC procedure on C18 RP column has been developed selected from different chemical groups: simazine, isoproturon, terbuthylazine, linuron, captan, terbutryn, procymidone, fenitrothion, clofentezine, bromopropylate. We investigated the possibility expanding scope four analyte extraction procedures isolation plant matrixes high levels...

The search for new substances with cytotoxic activity against various cancer cells, especially cells that are very resistant to currently used chemotherapeutic agents, such as melanoma is a important scientific aspect. We investigated the effect of Chelidonium majus, Mahonia aquifolium and Sanguinaria canadensis extracts obtained from different parts these plants collected at vegetation stages on FaDu, SCC-25, MCF-7, MDA-MB-231 cells. Almost all tested showed higher cytotoxicity than...

Different extraction and clean-up protocols, based on either the dispersive solid-phase (d-SPE) quick, easy, cheap, effective, rugged, safe (QuEChERS) approach, were optimized compared for determination of selected bisphenols such as bisphenol A (BPA) S (BPS) in breast milk samples. QuEChERS-based sample preparation procedure was by evaluation different sorbents including zirconium-based (Z-Sep Z-Sep Plus) primary secondary amine. Negligible matrix interference observed most analytes due to...

Background: Plants are an important origin of natural substances that the raw material for various pharmaceutical and therapeutic applications due to presence phytochemicals, such as alkaloids. Alkaloids, which found in different plant species, possess numerous biological activities. Some alkaloids have strong cytotoxic effects on cancer cells. The search new drugs treat cancers is one most challenges modern scientific research. Objective: This study aimed investigate activity extracts were...

Retention parameters of galantamine and lycorine standards were determined on different columns, i.e., octadecyl silica, SM C18, strong cation-exchange (SCX) columns with aqueous mobile phases. alkaloids was investigated C18 phase containing 5% MeCN, 20% acetate buffer at pH 3.5, 0.025 ML−1 diethylamine (DEA), SCX column 8% MeCN phosphate 2.5. Better results also obtained in ion-exchange chromatographic system. On the basis systems, simple, rapid, sensitive high-performance liquid...

Berberis species have a long history of use in traditional Chinese medicine, Ayurvedic and Western herbal medicine. The aim this study was the quantification main isoquinoline alkaloids extracts obtained from various by HPLC, vitro silico determination anti-cholinesterase activity, vivo investigations cytotoxic activity investigated plant alkaloid standards. In particular, whose had not been previously were selected for study. most extracts, high content berberine palmatine determined....

Polar-bonded stationary phases (CN-silica and Diol-silica) are used with nonaqueous eluents in adsorption mode or aqueous partition mode. This enables the application of these systems two-dimensional separations because different selectivity to separation closely related compounds similar physicochemical properties retention behaviour. Similarly, multiphase plates, connected C18 strips silica layers, eluents. Such layers were applied for selected coumarins. Thus, differences coumarin...