In this study, an acidophilic actinobacteria strain was used as a novel reducing agent for single-step synthesis of nanostructure silver particles. We Streptacidiphilus durhamensis HGG16n isolate efficient bioactive nanoparticles [bio(AgNPs)] in inexpensive, eco-friendly, and nontoxic manner. The obtained bio(AgNPs) exhibited unique physicochemical biochemical properties. Structural, morphological, optical properties the synthesized biocolloids were characterized by spectroscopy, dynamic...

Bisphenols may negatively impact human health. In this study, we propose the use of HPLC–FLD for simultaneous determination bisphenols in pericardial fluid samples collected from patients with coronary artery disease undergoing bypass surgery. For sample preparation, a fast, simple, and ”green” DLLME method was used, achieving mean recovery values range 62%–98% relative standard deviations between 2% 6% all analytes. Quantitative analysis then performed by LC–MS/MS on triple quadrupole (QqQ)...

The aim of the study was to neutralize zearalenone by lactic acid bacteria (LAB) such as Lactococcus lactis and Bifidobacterium sp. investigate mechanism (ZEA) binding. Neutralization ZEA LAB confirmed identification binding kinetics spectroscopic studies Fourier transform infrared spectroscopy (FT-IR) matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). obtained results showed that kinetic process L. is not homogeneous but expressed with an initial...

Changes in the metabolome of germinating seeds and seedlings caused by metal nanoparticles are poorly understood. In present study, effects bio-synthesized silver ((Bio)Ag NPs) on grains germination, early development, metabolic profiles roots, coleoptile, endosperm wheat were analyzed. Grains germinated well (Bio)Ag NPs suspensions at concentration range 10-40 mg/L. However, growth coleoptile was inhibited 25%, regardless tested, whereas roots gradually slowed down along with increasing...

Biologically active compounds are found in biological samples at relatively low concentration levels. The sample preparation of target from biological, pharmaceutical, environmental, and food matrices is one the most time-consuming steps analytical procedure. microextraction techniques dominant. Metabolomic studies also require application proper technique for determination endogenic metabolites present matrix on trace Due to reproducibility data, precision, cost appropriate analysis,...

In the present work acidophilic actinobacteria strain was used as a novel reducing agent for cheap, green and single-step synthesis of nanostructure silver particles. Structural, morphological optical properties synthesized nanoparticles have been characterized by spectroscopy, dynamic light scattering electron microscopy approach. The antimicrobial activity against clinical strains such Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Klebsiella...

This work reports the effect of silver bionanoparticles (Bio(AgNPs) synthesized by Actinobacteria CGG 11n on selected Gram (+) and (-) bacteria. Flow cytometry, classical antibiogram method fluorescent microscopy approach was used for evaluation antimicrobial activity Bio(AgNPs) their combination with antibiotics. Furthermore, performed research specified capacity flow cytometry as an alternative to standard ones a complementary electromigration techniques. The study showed antibacterial...

The resistance of pathogenic bacteria to antibiotics has become a serious problem. emphasis is placed on the development new, effective antimicrobial strategies. One them use AgNPs in association with antibiotic drugs. aim this study was obtain silver nanoparticles functionalized ampicillin and investigate mechanism binding nanoparticle using high-performance liquid chromatography approach. To confirm occurrence functionalization, FTIR, MALDI-TOF MS, DLS analysis zeta potential measurements...

This research presents, for the first time, potential of Lactobacillus paracasei LC20 isolated from sweet whey as a novel, effective and accessible source post-cultured ZnO nanocomposites synthesis. The obtained were subjected to comprehensive characterization by broad spectrum instrumental techniques. Results spectroscopic microscopic analysis confirmed hexagonal crystalline structure in nanometer size. dispersion stability was determined based on zeta (ZP) measurements—the average ZP value...

Abstract The effect of a different pH on Saccharomyces cerevisiae cells modified with calcium ions was investigated by the capillary zone electrophoresis technique. For identification wild strain S . , ribosomal nucleic acid sequencing and internal transcribed spacer as well spectrometric approach were applied. potentiometric titration Fourier transform infrared spectroscopy have shown occurrence active functional groups such carboxyl, amine/hydroxyl, phosphate/hydrogen phosphate surface...

In the present research, hybrid (AgCl, Ag)NPs/diatomite composites were synthesized by direct impregnation with aqueous silver nitrate solutions. The chloride nanoparticles (AgCl-NPs) formed as an effect of exchange reaction when interacted diatomite mineral impurity halite. Nanoparticles metallic (AgNPs) created reduction ions under influence hydrogen peroxide. content in composite was limited halite used diatomite. Samples natural and examined using scanning electron microscopy,...

The current work deals with the phenomenon of silver cations uptake by two kinds bacteria isolated from dairy products. mechanism sorption Lactococcus lactis and Lactobacillus casei was investigated. Inductively coupled plasma-mass spectrometry (ICP-MS) used for determination concentration sorbed bacteria. Analysis charge distribution conducted diffraction light scattering method. Changes in ultrastructure cells after treatment were investigated using transmission electron microscopy...

The zearalenone binding and metabolization ability of probiotic microorganisms, such as lactic acid bacteria, Lactobacillus paracasei, Lactococcus lactis, yeast Saccharomyces cerevisiae, isolated from food products, were examined. Moreover, the influence cellular stress (induced by silver nanoparticles) lyophilization on effectiveness tested microorganisms was also investigated. concentration after a certain time incubation with determined using high-performance liquid chromatography....

Abstract Cyclitols are phytochemicals naturally occurring in plant material, which attracted an increasing interest due to multiple medicinal attributes, among the most important antidiabetic, antioxidant, and anticancer properties. Due their valuable properties, sugars used food industry as sweeteners, preservatives, texture modifiers, fermentation substrates, flavoring coloring agents. In this study, we report for first time quantitative analysis of cyclitols isolated from Solidago...

The work presents the kinetic and isotherm studies of silver binding on casein, which was carried out using batch sorption technique. Moreover, influence light irradiation process shown. In order to investigate mechanism metal ions by casein zero, pseudo-first kinetics Weber-Morris intra-particle diffusion as well Langmuir Freundlich models were used. Furthermore, specify more precisely, possible mechanism, spectroscopic (FT-IR—Fourier Transform Infrared Spectroscopy, Raman), spectrometric...

In this study, for the first time, comparison of commercially available chemical ZnO NCs and bio-ZnO produced extracellularly by two different probiotic isolates (Latilactobacillus curvatus MEVP1 [OM736187] Limosilactobacillus fermentum MEVP2 [OM736188]) were performed. All types formulations characterized comprehensive interdisciplinary approach including various instrumental techniques in order to obtain nanocomposites with suitable properties further applications, i.e. biomedical. Based...