Changes in the metabolome of germinating seeds and seedlings caused by metal nanoparticles are poorly understood. In present study, effects bio-synthesized silver ((Bio)Ag NPs) on grains germination, early development, metabolic profiles roots, coleoptile, endosperm wheat were analyzed. Grains germinated well (Bio)Ag NPs suspensions at concentration range 10-40 mg/L. However, growth coleoptile was inhibited 25%, regardless tested, whereas roots gradually slowed down along with increasing...

The effect of the biological control agent Aureobasidium pullulans (de Bary) G. Arnaud on development Fusarium head blight (FHB) winter wheat and kernel contamination with fungi genera Fusarium, Acremonium, Cladosporium Penicillium was analyzed in a greenhouse experiment. Scanning electron microscopy used to evaluate distribution A. cells aggregates kernels, infection structures culmorum (W.G. Smith) Sacc antagonist-pathogen interactions. Biological reduced FHB severity by 21.67 % improved...

The analysis of the effects cadmium (Cd) on plant cells is crucial to understand defense mechanisms and adaptation strategies plants against Cd toxicity. In this study, we examined stress-related enzyme activities after one seven days application ultrastructure roots Pisum sativum L. treatment (10, 50, 100, 200 μM CdSO4). Our results showed that phenylalanine ammonia-lyase (PAL) activity amount accumulated in were significantly positively correlated with concentration used our experiment....

The impact of tetracycline on the soil and rhizosphere microbiome lettuce was analyzed. Soil collected from an agricultural field regularly fertilized with manure, added at two concentrations (5 mg/kg 25 mg/kg). In untreated soil, dominant bacteria included Proteobacteria (43.17%), Bacteroidota (17.91%), Firmicutes (3.06%). Tetracycline addition caused significant shifts in composition, notably increasing Actinobacteriota (22%) favoring Mycobacterium tuberculosis (low concentration)...

This paper describes, for the first time, effect of bacteria genus Sphingomonas on healthiness winter wheat. The application cell suspension development disease symptoms powdery mildew and Fusarium head blight (FHB) wheat cv. Bogatka was studied under greenhouse conditions. abundance populations yeast fungi producing mycelium as well Azotobacter pseudomonads determined kernels. biocontrol agent reduced population size poae, it contributed to better grain filling. tested isolate severity flag...

The difficulties related to treatment of deep surgical site infection involve formation biofilm on the surface synthetic material. It is considered that in infections involving biofilm, concomitant therapy shall be applied covering anti-inflammatory drugs. purpose work was assess impact diclofenac and ibuprofen bacterial monofilament polypropylene mesh.The study involved 70 strains Staphylococcus aureus Escherichia coli isolated from different patients those which differ with chromosomal DNA...

The phytotoxicity of silver nanoparticles (Ag NPs) to plant seeds germination and seedlings development depends on properties concentration, as well species stress tolerance degrees. In the present study, effect citrate-stabilized spherical Ag NPs (20 mg/L) in sizes 10, 20, 40, 60, 100 nm, wheat grain germination, early development, polar metabolite profile 3-day-old were analyzed. NPs, regardless their sizes, did not affect grains. However, smaller (10 20 nm size) decreased growth seedling...

Antibiotics are a new type of contaminants found in the environment. They increasingly used farm animal production systems and may accumulate crops, limiting plant growth rate nutritive value. The aim this study was to determine effects tetracycline (TC) on physiological biochemical properties pea seedlings. presence TC soil during 24 hours did not result any distinct changes However, after five days (120 h) action, seedling appearance metabolic activities were significantly affected. Leaves...

In wheat, resistance to Fusarium is conditioned by anatomical, morphological, and physiological traits. The aim of this study was evaluate selected elements constitutive barriers in common spelt, Polish emmer, einkorn. activity the phenylalanine ammonia-lyase (PAL) enzyme rate reactive oxygen species (ROS) production were evaluated tissues wheat spelt inoculated with culmorum. Most relict more abundant morphological than wheat. F. culmorum penetrated barriers, which increased PAL intensified...

The current study aimed to investigate the impact of silicon (Si) supplementation in form Na2SiO3 on metabolome peas under normal conditions and following exposure cadmium (Cd) stress. Si is known for its ability enhance stress tolerance various plant species, including mitigation heavy metal toxicity. Cd, a significant contaminant, poses risks both human health environment. focused analyzing levels bioactive compounds different parts, shoot, root, pod, understand influence their...

Abstract In the presented study, effects of cadmium (Cd) stress and silicon (Si) supplementation on pea plant ( Pisum sativum L.) were investigated. The tendency to accumulate in relevant morphological parts (roots shoots respectively)—bioaccumulation, transfer this element (translocation) physiological parameters through indicators oxidative determined. Model studies carried out at pH values 6.0 5.0 growth conditions hydroponic cultivation. It was shown that Cd accumulates mostly roots both...

Due to the high availability of lignocellulosic biomass, which can be obtained from terrestrial plants, agricultural waste and agro-food, paper or wood industries, its use for energy production by methane fermentation is economically environmentally justified. However, due their complex structures, substrates have a low conversion factor biogas. Therefore, scientists are still working on development new methods pre-treatment materials that will increase biogas productivity biomass. The...

Modern integrated farming systems encourage the search for alternative (non-chemical), highly effective methods of plant protection. In this study, fungistatic effect fennel essential oil (FEO) and hop (HEO) on fungal growth their ability to treat Fusarium wilt was investigated. The study conducted in vitro pot experiments. severity infection assessed by disease index (DI), presence culmorum gDNA (qPCR) anatomical analyses infected tissue using an optical (OM) scanning electron microscope...

This paper presents results of examination fruit surface in the genus Lamium L. (Lamiaceae) using scanning electron microscopy (SEM). The purpose this study was to define new features that differentiate taxa difficult identify on basis macromorphological features, such as species hybrid origin and subspecies within Lamium. our research show SEM analysis allowed defining diagnostic for almost all studied representatives except confertum from coutinhoi album subsp. barbatum. most useful...

Cadmium (Cd) as a toxic element has significant effect on plants. The presented study was focused the analysis of differences between short- (one day) and long-time (7 28 days) effects Cd (10, 50, 100 200 μM CdSO4) P. sativum L. showed that treatment generally reduced accumulation Ca, K Mn in pea elevation photosynthesis rate observed after one day treatment. However, opposite 7 days noticed, it similar for all concentrations used during experiment. influence application development delay...

A pot experiment on different methods of EM 1 application was conducted in the period 2002-2004. The study carried out under controlled growth conditions. experimental factor method EM1 application. Before application, biological preparation propagated as recommended by manufacturer (Greenland). health status aboveground parts 'Ramrod' pea plants estimated at flowering stage. assessment leaf gas exchange parameters (An, E, Gs, and Ci) made using a LI-Cor 6400 Portable Photosynthesis System....

Abstract The elemental composition and morphology of aerobic granules in sequencing batch reactors (GSBRs) treating high-nitrogen digester supernatant was investigated. investigation particularly focused on the effect number anoxic phases (one vs. two) cycle dose external organics loading (450 mg COD/(L·cycle) 540 COD/(L·cycle)) granule characteristics. Granules all were formed many single cells rod spherical bacteria. Addition second phase GSBR resulted enhanced settling properties about...

Photosynthesis is one of the main processes involved in plant growth and development.This process sensitive to environmental stressors, including cadmium environment contamination.Therefore, this study investigated, among other things, effect on fluorescence photosynthesis parameters, chlorophyll fluorescence; moreover, as carotenoids contents were measured.The toxic growth, development, was confirmed.In order binding mechanism by plant, first step verify quantification method with...

In this study, the ability of Lemna minor L. to recover normal growth, after being degraded in a tetracycline-containing medium, was extensively investigated. The plants were exposed tetracycline (TC) at concentrations 1, 2.5, and 10 mM. Subsequently, their physiological status analysed against following criteria: rate plant growth; free radical accumulation; antioxidant enzyme activity; chlorophyll content; HSP70 protein cell membrane permeability, mitochondrial activity. study showed that...

Seed vigour and viability, synchronous fluorescence spectroscopy, proteomic profiles were analysed in field bean (Vicia faba var. minor) seeds (two cultivars) subjected to dry storage at −14 °C or +20 for 30 years. The stored retained very high germinability (91–98%) until the end of experiments, while from same lots but room temperature completely lost viability. deterioration was also manifested by a vast (4- 6-fold) increase leachate electroconductivity, changes spectra profiles. To carry...