Volatile organic compounds (VOCs) have been studied in biological samples order to be related the presence of diseases. Sweat can represent substances existing blood, has less complex composition (compared with other matrices) and obtained a non-invasive way. In this work, sweat patches were collected from healthy controls volunteers cancer. Static Headspace was used for VOCs extraction, analysis performed by gas chromatography coupled mass spectrometry. Principal Components Analysis...

Halitosis and submandibular abscesses are examples of mouth-related diseases with the possible bacterial origin. Salivary volatile organic compounds (VOCs) potential biomarkers them, once they can be addressed as metabolites activity. Healthy patients (n = 15), subjects located in fascial deep space 10), halitosis 5) were enrolled study. Saliva samples subjected to headspace solid-phase microextraction (HS-SPME) gas chromatography coupled mass spectrometry (GC/MS) analysis. A total number...

Volatile organic compounds (VOCs) have been assessed in breath samples as possible indicators of diseases. The present study aimed to quantify 29 VOCs (previously reported potential biomarkers lung diseases) collected from controls and individuals with cancer, chronic obstructive pulmonary disease asthma. Besides that, global VOC profiles were investigated. A needle trap device (NTD) was used pre-concentration technique, associated gas chromatography-mass spectrometry (GC-MS) analysis....

The main goal of this study was to explore the phospholipid alterations associated with development prostate cancer (PCa) using two imaging methods: matrix-assisted laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF/MS), and electrospray triple quadrupole (ESI-QqQ/MS). For purpose, samples PCa tissue (n = 40) were evaluated in comparison controls 40). As a result, few classes compounds, namely phosphatidylcholines (PCs), lysophosphatidylcholines (LPCs), sphingomyelins...

Despite numerous studies, the molecular mechanism of prostate cancer development is still unknown. Recent investigations indicated that citric acid and lipids-with a special emphasis on fatty acids, steroids hormones (ex. prolactin)-play significant role in progression. However, assumed to be potential biomarker cancer, due which, diagnosis at an early stage disease could possible. For this reason, main goal study determine concentration three different matrices. To best our knowledge, first...

Staphylococcus aureus remains a major health problem responsible for many epidemic outbreaks. Therefore, the development of efficient and rapid methods studying molecular profiles S. strains its further typing is in high demand. Among techniques, matrix-assisted laser desorption ionization–time flight mass spectrometry (MALDI TOF MS) represents timely, cost-effective, reliable strain approach, which still rarely used due to insufficient knowledge about impact sample preparation analysis...

In this paper, a study of the cytotoxicity bare and functionalized zinc oxide nanoparticles (ZnO NPs) is presented. The ZnO NPs were obtained by various types biological methods including microbiological (intra- extracellular with Lactobacillus paracasei strain), phytochemical (Medicago sativa plant extract) biochemical (ovalbumin from egg white protein) synthesis. As control, gained chemical synthesis (commercially available) tested. was measured through use...

Understanding the changes that occur in milk during refrigeration is essential for optimizing dairy product processes and ensuring their quality shelf life. In this study, we aimed to investigate effects of a 7-day period on fatty acid (FA) profile protein composition milk. Our findings revealed significant alterations milk's FA after 7 days refrigeration. There was considerable decline 47% variety original FAs, accompanied by 12.5% reduction total content. Interestingly, decanoic...

Abstract Here we present application of innovative lab-made analytical devices such as plasmonic silver nanostructured substrates and polypyrrole-MOF solid-phase microextraction fibers for metabolic profiling bacteria. For the first time, comprehensive both volatile non-volatile low-molecular weight compounds in eight bacterial strains was carried out with utilization devices. Profiles low molecular metabolites were analyzed similarities differences using principal component analysis,...

The current study aimed to investigate the impact of silicon (Si) supplementation in form Na2SiO3 on metabolome peas under normal conditions and following exposure cadmium (Cd) stress. Si is known for its ability enhance stress tolerance various plant species, including mitigation heavy metal toxicity. Cd, a significant contaminant, poses risks both human health environment. focused analyzing levels bioactive compounds different parts, shoot, root, pod, understand influence their...

Abstract Cyclitols are secondary metabolites with bioactive properties, naturally occurring in plants. Accumulation of such is directly connected abiotic stressing factors. This article investigates the influence exercised by a series factors including plant treatment elicitors and cultivability various regions Europe, some cases during two seasons, onto amounts cyclitols produced alfalfa ( Medicago sativa L.). The obtained results highlighted that NaCl elicitation acts to increase quantity...

(1) Background: Little is known about the impact of urinary microflora, in particular, its effects on side after radiotherapy. The use mass spectrometry identification method (MALDI) may bring a new look at issue composition and significance microbiome. This study aimed to identify microbiome urine samples collected from 50 irradiated prostate cancer patients. (2) Methods: Blood were before gold marker implantation, start last day radiotherapy, 1, 4 months after. Patients do not always...

Escherichia coli and salivary Klebsiella oxytoca Staphylococcus saccharolyticus were subjected to different concentrations of silver nanoparticles (AgNPs), namely: 12.5, 50, 100 µg mL−1. Matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry (MALDI-TOF MS) spectra acquired after specified periods: 0, 1, 4, 12 h. For study volatile metabolites, headspace solid-phase microextraction coupled gas chromatography/mass (HS-SPME-GC-MS) was employed—AgNPs added bacteria cultures...

Considering the advent of antibiotic resistance, study bacterial metabolic behavior stimulated by novel antimicrobial agents becomes a relevant tool to elucidate involved adaptive pathways. Profiling volatile metabolites was performed monitor alterations metabolism induced biosynthesized silver nanoparticles (bio-AgNPs). Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae and Proteus mirabilis were isolated from pressure ulcers, their cultures prepared in presence/absence...

Abstract Ayahuasca, a hallucinogenic beverage used in religious rituals South America, has become global phenomenon. Its main active components are the β-carbolines alkaloids, harmine (HRM) and harmaline (HRL), as well potent hallucinogen N,N-dimethyltryptamine (DMT). Despite its rising consumption, information regarding possible clinical applications toxicological effects of ayahuasca is still limited. This study presents first investigation use sweat for determination DMT, HRM HRL users...