In the presented work identification of microorganisms isolated from various types honeys was performed. Martix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rDNA sequencing were applied to study environmental bacteria strains.With both approches, problematic spore-forming Bacillus spp, but also Staphylococcus spp., Lysinibacillus Micrococcus spp. Brevibacillus spp identified. However, application spectrometric technique allows for an...

The work presents the kinetic and isotherm studies of silver binding on casein, which was carried out using batch sorption technique. Moreover, influence light irradiation process shown. In order to investigate mechanism metal ions by casein zero, pseudo-first kinetics Weber-Morris intra-particle diffusion as well Langmuir Freundlich models were used. Furthermore, specify more precisely, possible mechanism, spectroscopic (FT-IR—Fourier Transform Infrared Spectroscopy, Raman), spectrometric...

The aim of this study was to investigate isolated β-lactoglobulin (β-LG) from the whey protein isolate (WPI) solution using column chromatography with SP Sephadex. physicochemical characterization (self-association, pH stability in various salt solutions, identification oligomeric forms) obtained have been carried out. electrophoretically pure β-LG fraction at 4.8. characterized by matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF/TOF MS) technique. use...

Abstract In this study, our primary objective was to develop an effective analytical method for studying trypsin-digested peptides of two proteins commonly found in cow's milk: β -casein ( CN) and -lactoglobulin LG). To achieve this, we employed distinct approaches: traditional in-gel protein digestion using immobilized enzyme microreactors (μ-IMER). Both methods utilized ZipTip pipette tips filled with C18 reverse phase media sample concentration. The μ-IMER fabricated through a multi-step...

Transmission and reflection data (0.5-5 eV) for 300 AA PdHx thin films (x=0.65, 0.85 0.92), hydrogenated under high-pressure conditions are presented compared with similar pure palladium before after hydrogen desorption. The main difference in transmission consists of a pronounced peak around 1 eV the structureless continuously increasing palladium. This confirms theoretical expectation Khan Riedinger (1982) that inter-band absorption edge PdH occurs at about eV. reflectivity were measurable...

Fundamental reflectivity spectra of CdxHg1-xTe in the 1.7-8.5 eV energy range have been investigated as a function temperature and Cd concentration. The thermal coefficients versus composition x for several observed maxima E1, E1+ Delta 1, E2, A B thresholds e1, e1+ Ea, Eb determined. On basis these results published theoretical experimental data an identification analysed transitions proposed.

The presented work is focused on the isotherm study Zn2+ ions binding to individual casein fractions: αS1-, β- and κ-casein (αS1CN, βCN κCN). experimental isotherms were evaluated using Freundlich Langmuir models. Subsequently, stability of obtained complexes in respective ion concentrations (120, 350, 600 mg/L) was determined by carrying out zeta potential measurements. Capillary electrophoresis combined with inductively coupled plasma mass spectrometry (CE-ICP-MS) confirmed occurring...

The drug-resistant pathogen phenomenon, resulting in infections and deaths that are increasingly difficult to treat, requires research into searching new potential antimicrobial agents. presented study is focused on the investigation of impact silver ions (Ag+ ions) β-lactoglobulin (βLG) structure mechanism formation silver-β-lactoglobulin nanocomposites, could find applications medicine. To determine physicochemical characteristics ion binding, kinetics isothermal models were used. presence...

The presented studies focused on the specificity binding of particular casein fractions: αS1-, β- and κ-casein (αS1CN, βCN, κCN), with zinc ions. mechanism was determined by kinetic modeling using results batch sorption. For this goal, models zero-order kinetics, pseudo-first-order, pseudo-second-order Weber–Morris intraparticle diffusion were used. formation Zn-αS1CN, Zn-βCN Zn-κCN complexes additionally monitored spectroscopic methods such as Fourier transform infrared spectroscopy (FT-IR)...

The room-temperature reflectivity spectra of Cd1-xMnxTe in the 0.5-30 eV energy range have been analysed. data display structures which can be attributed 0.5-11 to E0, E1, E1+ Delta 1, E2, E'1 and E'1+ transitions at several critical points zincblende-type semiconductors 10-30 Cd 4d core transitions. positions shifts above-indicated inter-band analysis allow for a quantitative estimate influence occupied empty Mn 3d states on optical Cd-Mn-Te system.

Abstract Measurements of positions the E 1 and l Δ fundamental reflection maxima for Cd x Hg − ;Te samples within composition range from  0 to 0.2 were performed. The relations ( ) [E + ]J found in this range. previous results referring same substances which had been questioned by otherinvestigators fully confirmed. An analysis all known until recently is presented.

Aim: The purpose of this study was to create an alternative protocol for the DNA-based identification salivary microbiota focused on distinguishing Streptococcus species. Materials & methods: Salivary bacteria were identified using 16S rDNA sequencing and proteins lipids profiling MALDI-TOF/MS as well FTIR analysis. Results: Most isolates belonged streptococci - mostly salivarious group indistinguishable by molecular technique. In turn, MALDI analysis allowed their fast reliable...

Abstract The width of the energy gap at A‐point Brillouin zone was measured in temperature range from 100 to 315 K for Cd x Hg 1‐ Te solid solutions. Measurements were carried out content 0 17%. thermal coefficients E l and + Δ transitions determined above mentioned composition. Some anomalies 1 ( T ) dependence have been found volume compositions x̄ = 0.125. A possible connection between these inversion band structure is discussed.

The present study reports on the in vivo application of (Bio)silver nanocomposite formulations (LBPC-AgNCs) wound healing. Additionally, emphasizes limited uptake silver by liver and blood tissues as well high viability PBMCs following external LBPC-AgNCs treatment. closure was monitored via stereoscopic microscope, a localization case tissue carried out (Inductively Coupled Plasma-Mass Spectrometers (ICP/MS), peripheral mononuclear cells (PMBC) determined flow cytometry technique....

The absorption coefficient of several diluted magnetic semiconductors was measured below the fundamental edge pure host compound at room temperature using photoacoustic technique. Several bands have been detected and assigned to electronic transitions between crystal field split 3d levels transition metal ion. In addition, weak thresholds observed ionization d states. An energy level diagram states has discussed.

The reflectivity and photoconductivity spectra for mixed crystals Cd1-xFexTe (0.00<or=x<or=0.05) have been investigated. measurements carried out in a wide energy range-1.0-30.0 eV at 300 77 K. taken the 1.0-3.0 range analysis of positions maxima as function Fe concentration allowed us to confirm existence deep donor-like state predicted by band-structure model these compounds. Using data value 0.08 above top valence band position this was found. influence Fe2+(3d6) states on changes E0, E1,...

Amorphous hydrogenated silicon-germanium alloys a-Si1−xGex:H in the form of thin films were prepared by rf sputtering and chemical vapor deposition methods with x varying from 0 to 0.63. Optical transmission, reflection, photoconductivity measurements visible near-infrared regions light enabled determination optical energy gap, band-tail states distribution, density defect near midgap. Dark conductivity measured temperature range 300–500 K. Electronic band structure (density versus energy)...