A5010623403- Lipid Membrane Structure and Behavior
- Protein Structure and Dynamics
- Advanced NMR Techniques and Applications
- Ion channel regulation and function
- Influenza Virus Research Studies
- Bone Tissue Engineering Materials
- Wound Healing and Treatments
- Computational Drug Discovery Methods
- Nicotinic Acetylcholine Receptors Study
- Spectroscopy and Quantum Chemical Studies
- Antimicrobial Peptides and Activities
- Enzyme function and inhibition
- RNA and protein synthesis mechanisms
- Periodontal Regeneration and Treatments
- Dermatologic Treatments and Research
- Electron Spin Resonance Studies
- Protein Hydrolysis and Bioactive Peptides
- Nanocomposite Films for Food Packaging
- Solid-state spectroscopy and crystallography
- Nanoplatforms for cancer theranostics
- Receptor Mechanisms and Signaling
- Medical Imaging and Analysis
- Laser Applications in Dentistry and Medicine
- Complex Network Analysis Techniques
- Nanoparticles: synthesis and applications
Pukyong National University
2016-2025
Florida State University
2007-2010
National High Magnetic Field Laboratory
2008-2010
Institute of Molecular Biology and Biophysics
2009
The M2 protein from the influenza A virus, an acid-activated proton-selective channel, has been subject of numerous conductance, structural, and computational studies. However, little is known at atomic level about heart functional mechanism for this tetrameric protein, a His(37)-Trp(41) cluster. We report structure conductance domain (residues 22 to 62) in lipid bilayer, which displays defining features native that have not attainable structures solubilized by detergents. propose cluster...
Speech impairments often emerge as one of the primary indicators Parkinson’s disease (PD), albeit not readily apparent in its early stages. While previous studies focused predominantly on binary PD detection, this research explored use deep learning models to automatically classify sustained vowel recordings into healthy controls, mild PD, or severe based motor symptom severity scores. Popular convolutional neural network (CNN) architectures, VGG and ResNet, well vision transformers, Swin,...
The M2 protein of influenza virus A is a proton-selective ion channel activated by pH. Structure determination solid-state and solution NMR X-ray crystallography has contributed significantly to our understanding, but activation may involve conformations not captured these studies. Indeed, data demonstrate that the possesses significant conformational heterogeneity. Here, we report molecular dynamics (MD) simulations transmembrane domain (TMD) in absence presence antiviral drug amantadine....
The mechanism of inhibition the influenza A virus M2 proton channel by antiviral drug amantadine has been under intense investigation. importance a mechanistic understanding is heightened prevalence amantadine-resistant mutations. To gain insight at molecular level, we carried out extensive dynamics simulations tetrameric in both apo and amantadine-bound forms lipid bilayer. simulation form revealed that Val27 from four subunits can secondary gate near entrance break water wire pore. This...
The process of silver immobilization onto and/or into bovine lactoferrin (LTF), the physicochemical properties and obtained silver-lactoferrin complexes, as well antibacterial activity complexes were investigated in this work. Kinetic study was carried out using batch sorption techniques. Spectrometric (MALDI-TOF/TOF-MS, ICP-MS), spectroscopic (FTIR, SERS), electron microscopic (TEM) electrophoretic (I-DE) techniques, zeta potential measurements, applied for characterization LTF binding...
Fish bone, a by‐product of fishery processing, is composed protein, calcium, and other minerals. The objective this study was to investigate the effects bioactive peptide isolated from bone marine fish, Johnius belengerii , on osteoblastic differentiation MC3T3‐E1 pre‐osteoblasts. Post consecutive purification by liquid chromatography, potent osteogenic peptide, 3 amino acids, Lys‐Ser‐Ala (KSA, MW: 304.17 Da), identified. purified promoted cell proliferation, alkaline phosphatase activity,...
The use of probiotics is considered an attractive biocontrol method. It effective in growth promotion aquaculture. However, the mode action fish terms remains unclear. objective present study was to investigate effect dietary administration host-derived probiotics, Lactococcus lactis WFLU12, on olive flounder compared control group fed with basal diet by analyzing their intestinal and serum metabolome using capillary electrophoresis mass spectrometry time-of flight (CE-TOFMS). Results...
Water wires are critical for the functioning of many membrane proteins, as in channels that conduct water, protons, and other ions. Here, liquid crystalline lipid bilayers under symmetric environmental conditions, selective hydrogen bonding interactions between eight waters comprising a water wire subset 26 carbonyl oxygens lining antiparallel dimeric gramicidin A channel characterized by 17O NMR spectroscopy at 35.2 T (or 1,500 MHz 1H) computational studies. While backbone 15N spectra...
In bone tissue regeneration, extracellular matrix (ECM) and bioceramics are important factors, because of their osteogenic potential cell–matrix interactions. Surface modifications with hydrophilic material including proteins show significant in engineering applications, scaffolds generally fabricated using synthetic polymers bioceramics. the present study, carbonated hydroxyapatite (CHA) marine atelocollagen (MC) were extracted from bones skins, respectively, Paralichthys olivaceus. The CHA...
This study presents a comprehensive comparison of U-Net variants with different backbone architectures for Macular Hole (MH) segmentation in optical coherence tomography (OCT) images. We evaluated eleven architectures, including combined InceptionNetV4, VGG16, VGG19, ResNet152, DenseNet121, EfficientNet-B7, MobileNetV2, Xception, and Transformer. Models were assessed using the Dice coefficient HD95 metrics on OIMHS dataset. While proved unreliable small regions like MH, often returning ‘nan’...
Restoring strength, function, and mobility following an illness, accident, or surgery is the primary goal of upper arm rehabilitation. Exoskeletons offer adaptable support, enhancing patient engagement accelerating recovery. This work proposes adjustable, wearable robotic exoskeleton powered by electromyography (EMG) data for Three activation levels-low, medium, high-were applied to EMG forecast Pulse Width Modulation (PWM) based on range motion (ROM) angle. Conventional machine learning...
Accurate retention time (RT) prediction in liquid chromatography remains a significant consideration molecular analysis. In this study, we explore the use of transformer-based language model to predict RTs by treating simplified input line entry system (SMILES) sequences as textual input, an approach that has not been previously utilized field. Our architecture combines pretrained RoBERTa (robustly optimized BERT approach, variant BERT) with bidirectional long short-term memory (BiLSTM)...
In this study, a marine microalga Spirulina sp.-derived protein was hydrolyzed using gastrointestinal enzymes to produce an angiotensin I (Ang I)-converting enzyme (ACE) inhibitory peptide. Following consecutive purification, the potent ACE peptide composed of 7 amino acids, Thr-Met‑Glu‑Pro‑Gly‑Lys-Pro (molecular weight, 759 Da). Analysis Lineweaver-Burk plot and molecular modeling suggested that purified acted as mixed non-competitive inhibitor ACE. The effects against cellular production...
A wealth of experimental data has verified the applicability Gouy-Chapman (GC) theory to charged lipid membranes. Surprisingly, a validation GC by molecular dynamics (MD) simulations been elusive. Here, we report test against extensive MD an anionic bilayer solvated water at different concentrations NaCl or KCl. We demonstrate that ion distributions from agree remarkably well with predictions when information on adsorption counterions is incorporated.
Nicotinic AChRs (nAChRs) represent a paradigm for ligand-gated ion channels. Despite intensive studies over many years, our understanding of the mechanisms activation and inhibition nAChRs is still incomplete. Here, we present molecular dynamics (MD) simulations alpha7 nAChR ligand-binding domain, both in apo form alpha-Cobratoxin-bound form, starting from respective homology models built on crystal structures acetylcholine-binding protein. The toxin-bound was relatively stable, its...
An analysis of scar tissue is necessary to understand the pathological conditions during or after wound healing process. Hematoxylin and eosin (HE) staining has conventionally been applied morphology tissue. However, lesions cannot be analyzed from a whole slide image. The current study aimed develop method for rapid automatic characterization in HE-stained tissues using supervised unsupervised learning algorithm. used Mask region-based convolutional neural network (RCNN) train pattern data...
Photodynamic therapy is an alternative approach to treating tumors that utilizes photochemical reactions between a photosensitizer and laser irradiation for the generation of reactive oxygen species. Currently, natural photosensitive compounds are being promised replace synthetic photosensitizers used in photodynamic because their low toxicity, lesser side effects, high solubility water. Therefore, present study investigated anti-cancer efficacy chlorophyllin-assisted on human cervical...