The use of probiotics is considered an attractive biocontrol method. It effective in growth promotion aquaculture. However, the mode action fish terms remains unclear. objective present study was to investigate effect dietary administration host-derived probiotics, Lactococcus lactis WFLU12, on olive flounder compared control group fed with basal diet by analyzing their intestinal and serum metabolome using capillary electrophoresis mass spectrometry time-of flight (CE-TOFMS). Results...

The existence of cancer stem cells (CSCs) is the main reason for failure treatment caused by drug resistance. Therefore, eradicating cancers targeting CSCs remains a significant challenge. In present study, because important role BMI-1 proto-oncogene, polycomb ring finger (BMI-1) and C-terminal Mucin1 (MUC1-C) in tumor growth maintenance CSCs, we aimed to confirm that microRNA miR-128, as an inhibitor MUC1-C, could effectively suppress paclitaxel (PTX)-resistant lung cells. We showed have...

In recent years, poikilothermic animals such as fish have increasingly been exposed to stressful high-temperature environments due global warming. However, systemic changes in under thermal stress are not fully understood yet at both the transcriptome and proteome level. Therefore, objective of this study was investigate immuno-physiological responses extreme through integrated multi-omics analysis. Trout were acute by raising water temperature from 15 25 °C within 30 min. Head-kidney plasma...

Bovine spongiform encephalopathy (BSE) is a progressive neurodegenerative disorder in cattle that poses risks to public health and has economic consequences. The shadow of prion protein encoded by the (SPRN) gene affects disease progression. A 12-base-pair indel polymorphism SPRN been implicated BSE susceptibility Polish cattle. Building upon our previous research, we aimed investigate polymorphisms Jeju black - Korean native breed with distinct genetic features compared them those Hanwoo...

ABSTRACT Viral hemorrhagic septicemia virus (VHSV) is a major pathogen in aquaculture worldwide, causing significant outbreaks through waterborne transmission. This study aimed to evaluate the effectiveness of environmental RNA (eRNA)‐based surveillance for detecting VHSV systems, focusing on relationship between infection dynamics, water temperature, and fish weight. Over an 8‐month period, prevalence was assessed outlet samples from six olive flounder farms Jeju Island, South Korea. Higher...

The nuclear factor κB (NF-κB) and interleukin-6 (IL-6) contribute to multidrug resistance (MDR) in tumor chemotherapy. essential phenomenon of oncogenic activation NF-κB cancer-initiating cells showing MDR resulting from increased IL-6 expression is still unclear. Cancer stem (CSCs) have been the objective intensive study. aim this study was investigate selective potential efficacy BRM270 against stem-like (SLCICs) via molecular mechanisms its anticancer effects. Co-regulation Cdk6 might be...

Tumor initiating cancer stem-like cells (TICSCs) have recently become the object of intensive study. Human-Lipocalin-2 (hLCN2) acts as a biomarker for cancers. The aim present study was to explore new insights regarding potential role LCN2 in inducing epithelial mesenchymal transition (EMT) by transfecting into CD133+-A549-TICSCs and its cross-talk with NF-κB signaling pathway adenocarcinoma lung. Furthermore, EMT confirmed transcriptomic analysis, immunoblotting immunocyto/histochemical...

Although over-nutrition from overfeeding-induced obesity is known to be highly associated with metabolic and immunological disorders in humans, little about fish farming. The purpose of this study was investigate changes immuno-physiological parameters, better understand the potential risk fish. Commercial feed provided overfed group until they refuse eat, but control fed at 1% bodyweight per day. hemato-serological, histological, were observed weeks 2 8. Rainbow trout leukocytes...

Abstract Background Mesenchymal stem cells (MSCs) are considered as an attractive approach for gene or drug delivery in cancer therapy. In the present study, ability of human bone marrow‐derived MSCs expressing cytosine deaminase/5‐fluorocytosine prodrug (CD/5‐FC MSCs) to target osteosarcoma cell line Cal72 was evaluated. Methods The stable CD/5‐FC MSC established by transfection pEGFP containing deaminase into with G418 selection. anti‐tumor effect verified a bystander assay vitro and...

Several efforts have been deployed to cure osteosarcoma, a high-grade malignant bone tumour in children and adolescents. However, some challenges such as drug resistance, relapse, metastasis remain owing the existence of cancer stem cells (CSC). There is an urgent need develop cost-effective safe therapies. Wogonin, extract from root Scutellaria baicalensis, has long considered promising natural compound for anti-tumourigenesis, particularly inhibit invasion metastasis. Hoechst 33,342...

Abstract Chemotherapy is a standard treatment for non-small-cell lung cancer (NSCLC). However, the dose-limiting toxicity of drugs and development chemoresistance are major clinical challenges to successful management NSCLC. Asian traditional medicine gaining global attention as non-toxic alternative chemotherapy. BRM270 an extract formulated from seven medicinal plants that has been shown inhibit tumor cell proliferation in diverse types. We previously demonstrated suppresses tumorigenesis...

In this study, we examined the effects of non-thermal dielectric barrier discharge plasma on embryonic development in chicken eggs order to determine optimal level exposure for promotion growth. We exposed developing embryos at either Hamburger-Hamilton (HH) stage 04 or HH 20 voltages 11.7 kV 27.6 kV. Our results show 1 min promoted development, but more duration and intensity resulted dose-dependent death survive longer than those 04. Furthermore, 4 increased production reactive oxygen...

Pancreatic cancer has a poor survival rate as compared to other types of cancer. Surface marker CD44 plays important role in epithelial-mesenchymal transition and stem cell phenotype. Therefore, targeting positive pancreatic cells might enhance therapies effectiveness. Our previous studies indicated the antitumorigenesis effect BRM270 osteosarcoma, lung cancer, glioblastoma; however there is no evidence on impacts growth. In this study, we investigated isolated ductal adenocarcinoma (CD44 +...

Abstract The quality of avian semen is an important economic trait in poultry production. present study examines the vitro effects non-thermal dielectric barrier discharge plasma on chicken sperm to determine conditions that can produce optimum quality. Exposure 11.7 kV for 20 s found maximum motility by controlling homeostasis reactive oxygen species and boosting release adenosine triphosphate respiratory enzyme activity mitochondria. However, prolonged exposure or further increase...

Abstract Non-thermal plasma treatment is an emerging innovative technique with a wide range of biological applications. This study was conducted to investigate the effect non-thermal dielectric barrier discharge on immature chicken Sertoli cell (SC) viability and regulatory role microRNA (miR)-7450. Results showed that increased SC apoptosis in time- dose-dependent manner. Plasma-induced possibly resulted from excess production reactive oxygen species via suppression antioxidant defense...

Background: This study was performed to identify the non-synonymous polymorphisms in myosin heavy chain 1 gene (MYH1) association with skeletal muscle development economically important Jeju Native Pig (JNP) and Berkshire breeds.Herein, we present an silico analysis, a focus on (a) approaches predict functional effect of SNP (nsSNP) MYH1 growth, (b) molecular docking dynamic simulation effects those nsSNP protein-protein association.Results: The NextGENe (V 2.3.4.) tool used variants from...

Pancreatic ductal adenocarcinoma (PDAC) is a major malignant phenotype in pancreatic cancer, which one of the most death causes by cancer world. PDAC developed from intra-epithelial neoplasms (PanINs) and poorly diagnosed at early stages. Beside high drug resistance, metastasis great concern during treatment. SALL4 expression inherent upregulations endothelial mesenchymal transition (EMT) genes therefore promoting metastasis. Furthermore, some evidences indicated reactive oxygen species...

Pigs are promising donors of biological materials for xenotransplantation; however, cell surface carbohydrate antigens, including galactose-alpha-1,3-galactose (α-Gal), N-glycolylneuraminic acid (Neu5Gc), and Sd blood group play a significant role in porcine xenograft rejection. Inactivating swine endogenous genes, GGTA1, CMAH, B4GALNT2, decreases the binding ratio human IgG/IgM peripheral mononuclear cells erythrocytes impedes effectiveness α-Gal, Neu5Gc, Sd, thereby successfully preventing...

As an innovative technology in biological applications-non-thermal plasma technique-has recently been applied to living cells and tissues. However, it is unclear whether non-thermal treatment can directly regulate the growth development of livestock. In this study, we exposed four-day-incubated fertilized eggs at 11.7 kV for 2 min, which was found be optimal condition respect highest rate chickens. Interestingly, plasma-treated male chickens conspicuously grew faster than females. Plasma...

Osteosarcoma (OS) is one of the most common malignant primary bone tumors and NF-κB appears to play a causative role, but mechanisms are poorly understood. OS pleomorphic, highly metastasized invasive neoplasm which capable generate osteoid, osteoclast osteoblast matrix. Its high incidence has been reported in adolescent children. Cell signal cascade pivotal functional mechanism acquired during differentiation, proliferation, growth survival cells including OS. The major limitation success...