A5078701789- Ion Transport and Channel Regulation
- Electrolyte and hormonal disorders
- Microbial Natural Products and Biosynthesis
- Enzyme Structure and Function
- Biochemical and Molecular Research
- Marine Sponges and Natural Products
- Hops Chemistry and Applications
- Neuroendocrine regulation and behavior
- Hormonal Regulation and Hypertension
- Enzyme Catalysis and Immobilization
- RNA and protein synthesis mechanisms
- Cancer Mechanisms and Therapy
- Herpesvirus Infections and Treatments
- Melanoma and MAPK Pathways
- Microtubule and mitosis dynamics
- Fermentation and Sensory Analysis
- Fungal and yeast genetics research
- ATP Synthase and ATPases Research
- Machine Learning in Bioinformatics
- Horticultural and Viticultural Research
- X-ray Diffraction in Crystallography
- Cardiomyopathy and Myosin Studies
- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Glioma Diagnosis and Treatment
Martinsburg VA Medical Center
2018
University of Oklahoma
2015-2017
National Cancer Institute
2015-2017
Center for Cancer Research
2015-2017
Target (United States)
2017
National Institutes of Health
1998-2015
Frederick National Laboratory for Cancer Research
2015
Hauptman-Woodward Medical Research Institute
2012-2014
University at Buffalo, State University of New York
2014
National Heart Lung and Blood Institute
1998-2000
Aldosterone stimulates sodium transport in the renal collecting duct by activating epithelial channel (ENaC). To investigate basis of this effect, we have developed a novel set rabbit polyclonal antibodies to 3 subunits ENaC and determined abundance distribution principal cells rat duct. Elevated circulating aldosterone (due either dietary NaCl restriction or infusion) markedly increased alphaENaC protein without increasing beta gamma subunits. Thus, is selectively induced aldosterone. In...
Although the collecting duct is regarded as primary site at which mineralocorticoids regulate renal sodium transport in kidney, recent evidence points to distal convoluted tubule a possible of mineralocorticoid action. To investigate whether expression thiazide-sensitive Na–Cl cotransporter (TSC), chief apical entry pathway cells, we prepared an affinity-purified, peptide-directed antibody TSC. On immunoblots, recognized prominent 165-kDa band membrane fractions from cortex but not medulla....
To investigate whether the enhancement of thick ascending limb (TAL) NaCl transport in response to long-term increases circulating vasopressin concentration is associated with increased expression levels apical Na-K-2Cl cotransporter rat TAL, we have carried out immunoblotting and immunofluorescence studies using affinity-purified, peptide-directed antibodies. Semiquantitative demonstrated a marked increase (193% controls) band density restriction water intake 15 ml/day for 7 days. In...
Sodium transport is increased by vasopressin in the cortical collecting ducts of rats and rabbits. Here we investigate, quantitative immunoblotting, effects on abundances epithelial sodium channel (ENaC) subunits (α, β, γ) rat kidney. Seven-day infusion 1-deamino-[8-d-arginine]-vasopressin (dDAVP) to Brattleboro markedly whole kidney β- γ-ENaC (to 238% 288% vehicle, respectively), whereas α-ENaC was more modestly, yet significantly, 142% vehicle). Similarly, 7-day water restriction...
Many bacteria use large modular enzymes for the synthesis of polyketide and peptide natural products. These multidomain contain integrated carrier domains that deliver bound substrates to multiple catalytic domains, requiring coordination these chemical steps. Nonribosomal synthetases (NRPSs) load amino acids onto through activity an upstream adenylation domain. Our lab recently determined structure engineered two-domain NRPS containing fused domains. This adopted a domain-swapped dimer...
The renal urea transporter gene (UT-A) produces different transcripts in the inner medullary collecting ducts (UT-A1) and thin descending limbs of Henle's loop (UT-A2), coding for distinct proteins. Peptide-directed rabbit polyclonal antibodies were used to identify UT-A2 protein medulla mouse rat. In stripe outer medulla, an antibody directed COOH terminus UT-A recognized a membrane 55 kDa. abundance this 55-kDa was strongly increased response chronic infusion vasopressin analog...
Mass-spectrometry-based metabolomics and molecular phylogeny data were used to identify a metabolically prolific strain of
Few secondary metabolites have been reported from mammalian microbiome bacteria despite the large numbers of diverse taxa that inhabit warm-blooded higher vertebrates. As a means to investigate natural products these microorganisms, an opportunistic sampling protocol was developed, which focused on exploring isolated roadkill mammals. This initiative made possible through establishment newly created discovery pipeline, couples laser ablation electrospray ionization mass spectrometry...
The addition of hops to fermented beer in the presence yeast may result a secondary fermentation commonly referred as "after-fermentation," "dry-hop creep," "ABV and "the freshening power hop" (FPH). effect is most likely caused by glycolytic enzyme(s) converting residual nonfermentable polysaccharides from malt into simple sugars that are then yeast. FPH must be considered because ethanol increase can exceed ±0.3% ABV tolerance set out U.S. Code Federal Regulations. Furthermore, with...
The adenosine monoposphate-forming acyl-CoA synthetase enzymes catalyze a two-step reaction that involves the initial formation of an acyl adenylate reacts in second partial to form thioester between substrate and CoA. These utilize Domain Alternation catalytic mechanism, whereby ∼110 residue C-terminal domain rotates by 140° distinct conformations for two reactions. structure acetoacetyl-CoA (AacS) is presented illustrates novel aspect this domain. Specifically, several acetyl- synthetases...
Mitogen-activated protein kinase (MAPK) p38 is part of a broad and ubiquitously expressed family MAPKs whose activity responsible for mediating an intracellular response to extracellular stimuli through phosphorylation cascade. central this signaling node activated by upstream kinases while being activating downstream transcription factors via phosphorylation. Dysregulated associated with numerous autoimmune disorders has been implicated in the progression several types cancer. A number...
The genomes of two fungi isolated from soil (MEA-2) and sediment (SUP5-1) were sequenced. Both members the order Hypocreales, closely related to Tolypocladium inflatum, capable producing novel secondary metabolites. draft enabled characterization key biosynthetic pathways.
ABSTRACT Protein subcellular localization is a critically important parameter to consider when designing expression constructs and production strategies for industry scale protein production. In this study, we present Prot-SCL an innovative self-supervised machine learning approach predict exclusively from primary sequence. The models herein were learned dataset of localizations derived by exhaustively analyzing the Uniprot database. set data was rigorously curated employing group sampling...