A5001477677- Mass Spectrometry Techniques and Applications
- Advanced Chemical Physics Studies
- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Molecular Spectroscopy and Structure
- Astrophysics and Star Formation Studies
- Ion-surface interactions and analysis
- Catalysis and Oxidation Reactions
- Molecular Junctions and Nanostructures
- Spectroscopy and Quantum Chemical Studies
- Analytical chemistry methods development
- DNA and Nucleic Acid Chemistry
- Gas Sensing Nanomaterials and Sensors
- Microtubule and mitosis dynamics
- Estrogen and related hormone effects
- Chemical Reaction Mechanisms
- Chemical Synthesis and Analysis
- Photochemistry and Electron Transfer Studies
- Advanced Photocatalysis Techniques
- Various Chemistry Research Topics
- Machine Learning in Materials Science
- Ga2O3 and related materials
- Catalytic Processes in Materials Science
- Cancer, Hypoxia, and Metabolism
University of Oklahoma
2016-2025
Sichuan University
2022-2025
University of Oklahoma Health Sciences Center
2023-2025
West China Hospital of Sichuan University
2025
OU Health Stephenson Cancer Center
2025
Tianjin University
2024
Xi'an Jiaotong University
2024
Anhui Medical University
2024
Guangdong Industry Technical College
2024
Heilongjiang University
2023
We have developed a new mass spectrometry (MS) technology, the Single-probe MS, capable of real-time, in situ metabolomic analysis individual living cells. The is miniaturized multifunctional sampling and ionization device that directly coupled to spectrometer. With tip smaller than eukaryotic cells (<10 μm), can be inserted into single sample intracellular compounds for real-time MS analysis. used detect several cellular metabolites anticancer small molecules paclitaxel, doxorubicin, OSW-1...
Copper plays vital roles in numerous cellular processes and its imbalance can lead to oxidative stress dysfunction. Recent research has unveiled a unique form of copper-induced cell death, termed cuproptosis, which differs from known death mechanisms. This process involves the interaction copper with lipoylated tricarboxylic acid cycle enzymes, causing protein aggregation death. Recently, growing number studies have explored link between cuproptosis cancer development. review comprehensively...
A novel architecture of nickel current collector (NCC) is fabricated cost-effectively by electrospinning. An ultrathin MnO2 coating electrodeposited on to the NCC and resultant nano-networks shell/NCC core are used as supercapacitor electrodes. Excellent electrochemical performance revealed at a high charge/discharge rate. The cyclic voltammetry measurements show that stable even scan rate fast 200 mV s−1. capacitance 214 F g−1 obtained galvanostatic testing density large 20 g−1, which much...
The exploration of single cells reveals cell heterogeneity and biological principle cellular metabolism. Although a number mass spectrometry (MS) based MS (SCMS) techniques have been dedicatedly developed with high efficiency sensitivity, limitations still exist. In this work, we introduced microscale multifunctional device, the T-probe, which integrates contents extraction immediate ionization, to implement online in situ SCMS analysis at ambient conditions minimal sample preparation. With...
Ambient mass spectrometry imaging (MSI) is an emerging field with great potential for the detailed spatial analysis of biological samples minimal pretreatment. We have developed a miniaturized sampling and ionization device, Single-probe, which uses in-situ surface micro-extraction to achieve high detection sensitivity resolution during MSI experiments. The Single-probe was coupled Thermo LTQ Orbitrap XL spectrometer able create MS images at 8 ± 2 8.5 μm on flat polycarbonate microscope...
Cancer stem cells (CSCs) are rare types of responsible for tumor development, relapse, and metastasis. However, current research in CSC biology is largely limited by the difficulty obtaining sufficient CSCs. Single-cell analysis techniques promising tools CSC-related studies. Here, we used Single-probe mass spectrometry (MS) technique to investigate metabolic features live colorectal CSCs at single-cell level. Experimental data were analyzed using statistical methods, including t-test...
A unique mass spectrometry (MS) method has been developed to determine the negatively charged species in live single cells using positive ionization mode. The utilizes dicationic ion-pairing compounds through miniaturized multifunctional device, single-probe, for reactive MS analysis of under ambient conditions. In this study, two reagents, 1,5-pentanediyl-bis(1-butylpyrrolidinium) difluoride (C5(bpyr)2F2) and 1,3-propanediyl-bis(tripropylphosphonium) (C3(triprp)2F2), were added solvent...
Abstract It is increasingly accepted that steroidal receptor coregulators may also function in the cytoplasmic compartment. Proline-, glutamic acid–, and leucine-rich protein-1 (PELP1) a novel coregulator plays role both genomic extranuclear actions of estrogen receptors (ER) hormonally responsive tissues. In this study using breast tumor arrays, we found PELP1 was localized only cytoplasm 58% PELP1-positive tumors. To help explain significance localization human tumors, created mutant...
Abstract The estrogen receptor plays an important role in breast cancer progression. Proline-, glutamic acid-, and leucine-rich protein 1 (PELP1), also called modulator of nongenomic activity (MNAR), a novel coactivator receptor, modulates transactivation functions. mechanisms by which PELP1 genomic functions is not known. Here, using biochemical scanning confocal microscopic analysis, we have demonstrated nuclear localization functional implications PELP1. Subnuclear fractionation showed...
Comparison between the gas-phase fragmentation of odd-electron M+*, [M + H]2+*, and - 2H]-* ions model peptides suggests that charge-remote radical-driven pathways play an important role in dissociation peptide ions. We have found processes are responsible for a variety side-chain losses from precursor ion some backbone fragmentation. These most likely involve hydrogen abstraction by radical site initiates subsequent cleavages. findings generally relevant to our understanding patterns...
The combination of single cell mass spectrometry with machine learning enables prediction drug-resistant phenotypes based on metabolomic profiles.
Traditional approaches for the assessment of physiological responses microbes in environment rely on bulk filtration techniques that obscure differences among populations as well individual cells. Here, were report development a novel micro-scale sampling device, referred to "Single-probe", which allows direct extraction metabolites from living, phytoplankton cells mass spectrometry (MS) analysis. The Single-probe is composed dual-bore quartz tubing pulled using laser pipette puller and...
Existing single cell mass spectrometry (SCMS) sampling platforms are largely designed to work only with immobilized cells and not the suspended isolated from patient samples. Here, we present a novel method that integrates commercially available manipulation system commonly used for in vitro fertilization Single-probe SCMS technology. The combined SCMS/cell manipulating platform is capable of rapidly analyzing intracellular species real time suspension leukemia line. A broad range molecular...
Analyzing cellular constituents on the single-cell level through mass spectrometry (MS) allows for a wide range of compounds to be studied simultaneously. However, there is need quantitative (qSCMS) methods fully characterize drug efficacy from individual cells within cell populations. In this study, qSCMS experiments were carried out using Single-probe MS technique. The method was successfully used perform rapid absolute quantifications anticancer irinotecan in mammalian cancer under...
Single cell MS (SCMS) techniques are under rapid development for molecular analysis of individual cells among heterogeneous populations. Lipids basic cellular constituents playing essential functions in energy storage and the signaling processes cells. Unsaturated lipids characterized with one or multiple carbon-carbon double (C═C) bonds, they critical human diseases. Characterizing unsaturated single allows better understanding metabolomic biomarkers therapeutic targets rare (e.g., cancer...