A5005368955- Genetic factors in colorectal cancer
- Histone Deacetylase Inhibitors Research
- Estrogen and related hormone effects
- Cancer-related molecular mechanisms research
- Cancer Cells and Metastasis
- Cancer-related Molecular Pathways
- Cancer-related gene regulation
- Lymphoma Diagnosis and Treatment
- Cytokine Signaling Pathways and Interactions
- Liver Disease Diagnosis and Treatment
- HER2/EGFR in Cancer Research
- Epigenetics and DNA Methylation
- Peptidase Inhibition and Analysis
- PI3K/AKT/mTOR signaling in cancer
- Cell Adhesion Molecules Research
- Liver Diseases and Immunity
- Adenosine and Purinergic Signaling
- Hedgehog Signaling Pathway Studies
- Cell death mechanisms and regulation
- Renal and related cancers
- Sperm and Testicular Function
- NF-κB Signaling Pathways
- Protein Kinase Regulation and GTPase Signaling
- Genetic Syndromes and Imprinting
- Cancer Genomics and Diagnostics
Southern Medical University Shenzhen Hospital
2017-2024
Xijing Hospital
2009-2017
Air Force Medical University
2008-2017
Guangdong General Hospital
2014
Guangdong Academy of Medical Sciences
2014
University of Nebraska Medical Center
2014
Shandong University
2014
George Washington University
2013
Rajiv Gandhi Centre for Biotechnology
2013
University of Cincinnati
2013
Stimulation of growth factor signaling has been implicated in the development invasive phenotypes and activation p21-activated kinase (Pak1) human breast cancer cells (Adam, L., Vadlamudi, R., Kondapaka, S. B., Chernoff, J., Mendelsohn, Kumar, R. (1998) <i>J. Biol. Chem.</i> 273, 28238–28246; Adam, Mandal, M., (2000)<i>J. 275, 12041–12050). To study role Pak1 regulation motility epithelial cells, we developed MCF-7 clones that overexpressed kinase-active T423E mutant under an inducible...
p21-activated kinase 1 (Pak1) has been shown recently to induce hyperplasia in the mammary epithelium, a phenotype also manifested by overexpression of cyclin D1, known indicator proliferative stage. Here we investigated role Pak1 pathway expression D1 using tissue culture models and transgenic mice expressing activated glands. We found that hyperplastic glands from catalytically active exhibit 5- 7-fold increased as compared with stage-matched wild-type mice. In addition, levels were...
Nuclear hormone receptors (NRs) are transcription factors whose activity is regulated by ligands and coactivators or corepressors. We report the characterization of a new NR coregulator: proline-, glutamic acid-, leucine-rich protein 1 (PELP1), novel human that comprises 1,282 amino acids localized on chromosome 17. The primary structure PELP1 consists several motifs present in most transcriptional regulators including nine NR-interacting boxes (LXXLL motifs), zinc finger, acid- proline-rich...
The MYC oncogene is overexpressed in hepatocellular carcinoma (HCC) and has been associated with widespread microRNA (miRNA) repression; however, the underlying mechanisms are largely unknown. Here, we report that c-Myc oncogenic transcription factor physically interacts enhancer of zeste homolog 2 (EZH2), a core enzymatic unit polycomb repressive complex (PRC2). Furthermore, miR-101, an important tumor-suppressive miRNA human hepatocarcinomas, epigenetically repressed by PRC2 c-Myc-mediated...
Germ cell degeneration is common in mammalian testes during the developmental as well adult period. To investigate extent and mechanisms of male germ death fetal neonatal life, mice at various postnatal ages extending from 13 days gestation to 7 wk after birth were examined by electron microscopy and/or terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL). Electron revealed that number cells with typical features spermatogenic apoptosis was highest gestation,...
Abstract Here, we investigated the role of P21-activated kinase 1 (Pak1) signaling in function estrogen receptor-α (ER-α) as assessed by serine 305 (S305) activation and transactivation activity ER. We found that Pak1 overexpression interfered with antiestrogenic action tamoxifen upon ER hormone-sensitive cells. In addition, stimulation led to up-regulation target genes breast cancer cells increased expression. Tamoxifen also Pak1-ER interaction tamoxifen-resistant but not...
Abstract Cancer has been considered to be the result of accumulated gene mutations, which in uncontrolled cell proliferations for a long time. Cancers are also regarded capable immune evasion. Furthermore, resistance apoptosis was recognized as an important trait cancer last score years. However, there numerous paradoxical issues this whole set theory. For example, is no known genes mutations responsible human cancers. As ‘resistance apoptosis’, fact that increased frequency apoptosis. The...
Etk/Bmx, a member of the Tec family nonreceptor protein-tyrosine kinases, is characterized by an N-terminal pleckstrin homology domain and has been shown to be downstream effector phosphatidylinositol 3-kinase. P21-activated kinase 1 (Pak1), another well 3-kinase, implicated in progression breast cancer cells. In this study, we role Etk mammary development tumorigenesis explored functional interactions between Pak1. We report that expression developmentally regulated gland. Using transient...
Epidermal growth factor (EGF) family of factors and their receptors regulate normal cancerous epithelial cell proliferation, a process that can be suppressed by antireceptor blocking antibodies. To identify genes whose expression may modulated antibodies, we performed differential display screen with cells grown in the presence or absence antibodies; isolates from one cDNA clone were 100% identical to human heterogeneous nuclear ribonucleoprotein K (hnRNP K), protein conserved KH motif RGG...
Heregulin-β1 promotes the activation of p21-activated kinase 1 (Pak1) and motility invasiveness breast cancer cells. In this study, we identified vascular endothelial growth factor (VEGF) as a gene product induced by heregulin-β1. The stimulation heregulin-β1 epithelial cells expression VEGF mRNA protein its promoter activity. also stimulated angiogenesis in VEGF-dependent manner. Herceptin, an anti-HER2 antibody inhibited heregulin-β1-mediated both Because Pak1 are positively regulated...
Emerging data suggest that metastasis-associated protein 1 (MTA1) represses ligand-dependent transactivation functions of estrogen receptor-alpha in cultured breast cancer cells and MTA1 is upregulated human tumors. However, the role tumorigenesis a physiologically relevant animal system remains unknown. To reveal mammary gland development, transgenic mice expressing under control mouse tumor virus promoter long terminal repeat were generated. Unexpectedly, we found glands these virgin...
Airway remodeling is a repair process that occurs after injury resulting in increased airway hyper-responsiveness asthma. Thymic stromal lymphopoietin (TSLP), vital cytokine, plays critical role orchestrating, perpetuating and amplifying the inflammatory response TSLP also factor asthma.To examine of TSLP-induced cellular senescence asthma vitro vivo.Cellular were examined lung specimens from patients with using immunohischemical analysis. Both small molecule shRNA approaches target...
Overexpression of the prometastatic chromatin modifier protein metastasis tumor antigen 1 (MTA1) in human cancer contributes to aggressiveness, but role endogenous MTA1 has not been explored. Here, we report effects selective genetic depletion a physiologically relevant spontaneous mouse model breast pulmonary metastasis. We found that acts as mandatory breast-to-lung without on primary formation. The underlying mechanism involved MTA1-dependent stimulation STAT3 transcription through action...
To elucidate the molecular mechanisms by which human epidermal growth factor receptor/heregulin (HER2/HRG) influence migratory potential of breast cancer cells, we have used phospho-specific antibodies against c-Src kinase and focal adhesion (FAK). This study establishes that HER2/HRG signaling selectively upregulates Tyr phosphorylation at Tyr-215 located within SH2 domain, increases activity FAK Tyr-861. HER2-overexpressing tumors showed increased levels Tyr-215. These findings suggest...
Abstract Purpose: Abnormalities in the expression and signaling pathways downstream of epidermal growth factor receptor (EGFR) contribute to progression, invasion, maintenance malignant phenotype human cancers, including those head neck breast. Accordingly, agents such as EGFR tyrosine kinase inhibitor (EGFR-TKI) ZD1839 (Iressa) are promising, biologically based treatments that various stages preclinical clinical development. The process tumor progression requires, among other steps,...
Here, we provide gain-of-function, loss-of function, and molecular evidence supporting genetic interactions between metastasis associated protein 1 (MTA1) Six3 rhodopsin. We discovered that MTA1 physically interacts with the chromatin in a histone deacetylase-dependent manner, leading to transcriptional suppression of gene. is also Six3-interacting corepressor contributes self-negative regulation transcription by Six3. In contrast, deletion alleles murine embryonic fibroblasts or its...
Proline-, glutamic acid-, and leucine-rich protein-1 (PELP1) is a novel estrogen receptor coactivator that plays an important role in the genomic nongenomic actions of by interacting with histones src-mitogen-activated protein kinase pathway, respectively. A great deal information has emerged recent years about possible PELP1 signaling. However, participation significance other cellular signaling pathways remains unknown. Using yeast two-hybrid screen, we identified as signal transducers...
Gain-of-function mutations of enhancer Zeste homolog 2 (EZH2) occur frequently in diffuse large B-cell lymphomas and follicular lymphomas. However, the frequency EZH2 mutation Chinese potential targets affected by this are unknown.We determined codon 641 (n = 124) compared them with Western 70) using a sensitive pyrosequencing assay. Gene expression profiling (GEP) was performed to determine differential gene between mutated versus unmutated subgroups, selected genes were validated...
Abstract Cancers are thought to be the result of accumulated gene mutations in cells. Carcinomas, which cancers arising from epithelial tissues usually go through several stages development: atypical hyperplasia, carcinoma situ and then invasive carcinoma, might further metastasize. However, we think that present pathological data enough prove there an alternative way carcinogenesis. We propose majority arise connective tissue stroma de novo, misplaced stem cells come wrong land by accident....