A5040130639- Estrogen and related hormone effects
- Reproductive System and Pregnancy
- Cancer Cells and Metastasis
- Cancer Risks and Factors
- Breast Cancer Treatment Studies
- Biochemical and Molecular Research
- Reproductive Biology and Fertility
- Effects and risks of endocrine disrupting chemicals
- Bone fractures and treatments
- Regulation of Appetite and Obesity
- Orthopedic Surgery and Rehabilitation
- Menopause: Health Impacts and Treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Eosinophilic Esophagitis
- Genomics and Chromatin Dynamics
- Receptor Mechanisms and Signaling
- Adipose Tissue and Metabolism
- Acute Lymphoblastic Leukemia research
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- RNA regulation and disease
- 14-3-3 protein interactions
- Cellular Mechanics and Interactions
Combined Military Hospital
2024
Deccan School of Hospital Management
2024
University of Cincinnati
2005-2023
University of Cincinnati Medical Center
2012-2023
The University of Texas Southwestern Medical Center
2023
Parkview Medical Center
2022
Dow University of Health Sciences
2022
Civil Hospital Karachi
2022
Institute of Space Technology
2022
University of Veterinary and Animal Sciences
1997-2021
Our data demonstrate that estrogens, estrogen receptor-α (ERα), and receptor-β (ERβ) regulate adipose tissue distribution, inflammation, fibrosis, glucose homeostasis, by determining αERKO mice have increased inflammation fibrosis prior to obesity onset. Selective deletion of ERα in adult using a novel viral vector technology recapitulated the findings total body null mice. Generation mouse model, lacking specifically from adipocytes (AdipoERα), demonstrated markers especially males....
The estrogen receptor-α (ERα) is a critical transcription factor that regulates epithelial cell proliferation and ductal morphogenesis during postnatal mammary gland development. Tissue recombination transplantation studies using the first generation of ERα knockout (ERKO) mice suggested this steroid hormone receptor required in stroma subsequently exerts its effect on epithelium through additional paracrine signaling events. A more detailed analysis revealed ERKO produce truncated protein...
We have determined the nucleotide sequence of a functional mouse adenine phosphoribosyltransferase (APRT) gene and its cDNA. The amino acid enzyme is deduced from an open reading frame in cDNA predicts protein with molecular weight 19,560. coding region approximately 2 kilobases, it composed five exons four introns. While body 53% G + C, 200 nucleotides upstream ATG translation start codon are 66% C contain three copies C-C-G-C-C-C. APRT shares homologous 20-amino mouse, hamster, human...
ABSTRACT Reduced bioavailability of estrogen increases skeletal fracture risk in postmenopausal women, but the mechanisms by which regulates bone mass are incompletely understood. Because signaling acts, part, through receptor alpha (ERα), mice with global deletion ERα (ERαKO) have been used to determine role biology. These animals, however, confounding systemic effects arising from other organs, such as increased and decreased insulin-like growth factor 1 (IGF-1) serum levels, may...
Binge eating afflicts approximately 5% of US adults, though effective treatments are limited. Here, we showed that estrogen replacement substantially suppresses binge-like behavior in ovariectomized female mice. Estrogen-dependent inhibition was blocked mice specifically lacking receptor-α (ERα) serotonin (5-HT) neurons the dorsal raphe nuclei (DRN). Administration a recently developed glucagon-like peptide-1-estrogen (GLP-1-estrogen) conjugate designed to deliver GLP1 receptor-enhanced...
Estrogen receptor–α (ERα) activity in the brain prevents obesity both males and females. However, ERα-expressing neural populations that regulate body weight remain to be fully elucidated. Here we showed single-minded–1 (SIM1) neurons medial amygdala (MeA) express abundant levels of ERα. Specific deletion gene encoding ERα (Esr1) from SIM1 neurons, which are mostly within MeA, caused hypoactivity male female mice fed with regular chow, increased susceptibility diet-induced (DIO) but not...
Abstract Here, we investigated the role of P21-activated kinase 1 (Pak1) signaling in function estrogen receptor-α (ER-α) as assessed by serine 305 (S305) activation and transactivation activity ER. We found that Pak1 overexpression interfered with antiestrogenic action tamoxifen upon ER hormone-sensitive cells. In addition, stimulation led to up-regulation target genes breast cancer cells increased expression. Tamoxifen also Pak1-ER interaction tamoxifen-resistant but not...
Estrogen receptor α (ERα) plays an important role in the onset and progression of breast cancer, whereas p53 functions as a major tumor suppressor. We previously reported that ERα binds to p53, resulting inhibition transcriptional regulation by p53. Here, we report on molecular mechanisms which suppresses p53’s transactivation function. Sequential ChIP assays demonstrated represses p53-mediated activation human cancer cells recruiting nuclear corepressors (NCoR SMRT) histone deacetylase 1...
The bulk alignment of actin filament sliding movement, powered by randomly oriented myosin molecules, has been observed and studied using an in vitro motility assay. well established, gliding assay is a minimal experimental system for studying actomyosin motility. Here, we show that when the performed at densities filaments approaching those found living cells, takes up preferred orientation. patterns movement have extend over length scale 10-100 microm, similar to size mammalian cell. We...
Humans show significant sex differences in the incidence and severity of respiratory diseases, including asthma virus infection. Sex hormones contribute to female bias type 2 inflammation associated with consistent recent reports that lungs harbor greater numbers GATA-3-dependent group innate lymphoid cells (ILC2s). In this study, we determined whether hormone levels govern numbers, phenotype, function ILC2s murine lung bone marrow (BM). Our data mice significantly ILC2 homeostasis, part due...
Brain glucose-sensing neurons detect glucose fluctuations and prevent severe hypoglycemia, but mechanisms mediating functions of these are unclear. Here we report that estrogen receptor-α (ERα)-expressing in the ventrolateral subdivision ventromedial hypothalamic nucleus (vlVMH) can sense fluctuations, being glucose-inhibited (GI-ERα
Abstract Hypercholesterolemia and vascular inflammation are key interconnected contributors to the pathogenesis of atherosclerosis. How hypercholesterolemia initiates is poorly understood. Here we show in male mice that hypercholesterolemia-driven endothelial activation, monocyte recruitment atherosclerotic lesion formation promoted by a crosstalk between macrophages cells mediated cholesterol metabolite 27-hydroxycholesterol (27HC). The pro-atherogenic actions macrophage-derived 27HC...
Evidence is presented that the estrogen antagonist 4-hydroxytamoxifen (HT) can occupy not only core binding pocket within ligand-binding domain of receptor (ER) beta but also a second site on its surface. The crystal structure (LBD) associated with HT was determined to 2.2 A and revealed two molecules bound protein. One located in consensus pocket, whereas other overlaps hydrophobic groove coactivator recognition Relative ERalpha-tamoxifen structure, helix 12 has been displaced from surface...
Previous studies using <i>in vitro</i> procedures have not clearly established whether the estrogen receptor (ER) acts as a monomer or dimer in cell. We used yeast two-hybrid system an vivo</i> approach to investigate dimerization of absence and presence anti-estrogens. This is independent ER binding response element. Two vectors, expressing GAL4 DNA domain-human transactivation ER, were constructed. Control experiments showed that each fusion protein had high affinity site for estradiol-17β...
Mediator recently has emerged as a central player in the direct transduction of signals from transcription factors to general transcriptional machinery. In case nuclear receptors, vitro studies have shown that coactivator function involves ligand-dependent interactions MED1 subunit, through its two classical LxxLL motifs, with receptor AF2 domain. However, despite strong evidence, there currently is little information regarding vivo functions motifs either or other coactivators. Toward this...
Although transcriptional activation by NF-κB is well appreciated, physiological importance of repression in cancer has remained elusive. Here we show that an HDAC4–RelB–p52 complex maintains repressive chromatin around proapoptotic genes Bim and BMF regulates multiple myeloma (MM) survival growth. Disruption RelB–HDAC4 a HDAC4-mimetic polypeptide blocks MM RelB-p52 also represses translation regulating miR-221 expression. While the NIK-dependent been reported, regardless status NIK oncogenic...
Gastric schwannomas (GS) are very rare spindle cell, submucosal mesenchymal tumors that arise from Schwann cells of nerve plexuses in the stomach wall. They usually benign but can become malignant and metastasize to other organs. Surgical resection with biopsy is gold standard for diagnosis management GS. In this article, we present a 68-year-old female patient who presented abdominal pain, nausea, vomiting, belching couple months. Upon further evaluation, she was found have 4.2 cm gastric...