A5017915016- Chronic Lymphocytic Leukemia Research
- Multiple Myeloma Research and Treatments
- DNA and Nucleic Acid Chemistry
- Immune Cell Function and Interaction
- Cell death mechanisms and regulation
- Bacterial Genetics and Biotechnology
- Diabetes and associated disorders
- S100 Proteins and Annexins
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Phagocytosis and Immune Regulation
- Phytochemicals and Medicinal Plants
- Lymphoma Diagnosis and Treatment
- Kruppel-like factors research
- Autophagy in Disease and Therapy
- Peptidase Inhibition and Analysis
- Chronic Myeloid Leukemia Treatments
- Immunodeficiency and Autoimmune Disorders
- Adipose Tissue and Metabolism
- Acute Lymphoblastic Leukemia research
- Gut microbiota and health
- Papaya Research and Applications
- PARP inhibition in cancer therapy
- Immune Response and Inflammation
- Andrographolide Research and Applications
- Antiplatelet Therapy and Cardiovascular Diseases
Western University of Health Sciences
2021-2023
Markey Cancer Center
2017-2022
University of Kentucky
2017-2022
University of Alabama at Birmingham
2018-2020
University of Cincinnati Medical Center
2015
University of Cincinnati
2013
Tata Institute of Fundamental Research
2008-2009
Myocardial infarction (MI) triggers myelopoiesis, resulting in heightened production of neutrophils. However, the mechanisms that sustain their and recruitment to injured heart are unclear. Using a mouse model permanent ligation left anterior descending artery flow cytometry, we first characterized temporal spatial effects MI on different myeloid cell types. We next performed global transcriptome analysis cardiac types within infarct identify drivers acute inflammatory response underlying...
There is incomplete knowledge of the impact bone marrow cells on gut microbiome and barrier function.We postulated that diabetes mellitus systemic ACE2 (angiotensin-converting enzyme 2) deficiency would synergize to adversely both function.Bacterial 16S rRNA sequencing metatranscriptomic analysis were performed fecal samples from wild-type, ACE2-/y, Akita (type 1 mellitus), ACE2-/y-Akita mice. Gut integrity was assessed by immunofluorescence, cell extravasation into small intestine evaluated...
The induction of tumor suppressor proteins capable cancer cell apoptosis represents an attractive option for the re-purposing existing drugs. We report that anti-malarial drug, chloroquine (CQ), is a robust inducer Par-4 secretion from normal cells in mice and patients clinical trial. CQ-inducible triggers paracrine also inhibits metastatic growth. CQ induces via classical secretory pathway requires activation p53. Mechanistically, p53 directly Rab8b, GTPase essential vesicle transport to...
Although transcriptional activation by NF-κB is well appreciated, physiological importance of repression in cancer has remained elusive. Here we show that an HDAC4–RelB–p52 complex maintains repressive chromatin around proapoptotic genes Bim and BMF regulates multiple myeloma (MM) survival growth. Disruption RelB–HDAC4 a HDAC4-mimetic polypeptide blocks MM RelB-p52 also represses translation regulating miR-221 expression. While the NIK-dependent been reported, regardless status NIK oncogenic...
Abstract Chronic lymphocytic leukemia (CLL) patients progressively develop an immunosuppressive state. CLL have more plasma IL-10, anti-inflammatory cytokine, than healthy controls. In vitro human cells produce IL-10 in response to BCR cross-linking. We used the transgenic Eμ–T cell oncogene-1 (TCL1) mouse model study role of associated immunosuppression. Eμ-TCL mice spontaneously because a B cell–specific expression oncogene, TCL1. Eμ-TCL1 constitutively which is further enhanced by...
We sought to delineate the retinal features associated with high-fat diet (HFD) mouse, a widely used model of obesity. C57BL/6 mice were fed either (60% fat; HFD) or low-fat (10% LFD) for up 12 months. The effect HFD on body weight and insulin resistance measured. retina was assessed by electroretinogram (ERG), fundus photography, permeability studies, trypsin digests enumeration acellular capillaries. cohort experienced hypercholesterolemia when compared LFD cohort, but not hyperglycemia....
Multiple Myeloma (MM) is an incurable plasma cell cancer that caused by several chromosomal translocations and gene deletions. Although deregulation of signaling pathways including the Nuclear Factor-Kappa B (NF-κB) pathway has been reported in MM, molecular requirement crosstalk between NF-κB its target genes MM survival largely unclear. Here, we report Yin Yang1 (YY1), a for NF-κB, hyperexpressed most tumor cells obtained from human patients, exhibits constitutive nuclear localization,...
// Karine Z. Oben 1 , Sara S. Alhakeem Mary K. McKenna Jason A. Brandon 2 Rajeswaran Mani 3 Sunil Noothi Liu Jinpeng 4 Shailaja Akunuru 5 Sanjit Dhar 6 Inder P. Singh 7 Ying Liang Chi Wang Ahmed Abdel-Latif Harold F. Stills Jr 8 Daret St. Clair Hartmut Geiger Natarajan Muthusamy Kaoru Tohyama 9 Ramesh C. Gupta 10 and Subbarao Bondada Markey Cancer Center Department of Microbiology, Immunology Molecular Genetics, University Kentucky, Lexington, KY 40536, USA Internal Medicine, Comprehensive...
We report a study of dynamics with dsDNA-specific dye called PicoGreen bound to plasmid DNA (3.4 kb), and show that at low dye/DNA phosphate ratios (1 : 100 below), reflect the motional dsDNA. further evaluated usefulness this probe by measuring time-resolved fluorescence dsDNA in presence cationic reagents affect [MgCl2 polyethyleneimine (PEI)] also different topological states. Among these conditions, MgCl2, PEI supercoiled form resulted increases very short component (0.2-0.4 ns)...
Prostate apoptosis response-4 (Par-4) is a tumor suppressor that induces in cancer cells. However, the physiological function of Par-4 remains unknown. Here we show conventional knockout (Par-4
MutS and UvrD proteins individually stimulate Escherichia coli exonuclease VII activity on blunt-ended short duplex DNA substrates. Stimulation by both is ATP-dependent but not mismatch-specific accompanied apparent strand separation. Under similar conditions, in fact confer resistance to action ssDNA targets, thereby implying that a novel state of double-stranded intermediate, which we term "destabilized duplex", involved exonuclease-mediated degradation. We find strands such destabilized...
Abstract Chronic Lymphocytic Leukemia (CLL), the most common adult leukemia in western world, is characterized by accumulation of clonally expanded CD5+CD19+ B lymphocytes peripheral blood and secondary lymphoid organs. CLL cell survival dependent on receptor signaling cross-talk within microenvironment. presents with enlargement spleen, lymph nodes due to long-lived impaired apoptotic mechanisms. The Eμ-Tcl1 mice, a specific overexpression oncogene, T 1 (Tcl1), serve as an excellent model...
Abstract Chronic Lymphocytic Leukemia (CLL), the most prevalent adult leukemia, is characterized by clonal expansion of CD5+ CD19+ B cells within lymphoid organs and accumulation quiescent in blood. Eμ-Tcl1 transgenic mice expressing Tcl1 oncogene a cell-specific manner develop CLL adoptive transfer from these induces growth C57BL/6 mice. These models typically cause splenomegaly as burden increases, localize mainly spleen first 10 days post-injection. We hypothesized that splenic...
The chronic lymphocytic leukemia (CLL) microenvironment has been receiving an increasing amount of attention, but there is currently limited data surrounding how the affects initial development CLL. We determined that spleen site CLL growth through monitoring transgenic Eμ-TCL1 mice develop Subsequently, we isolated stromal cells from spleens (EMST cells) induce cell division in vitro. Both cell-cell contact and soluble factors were involved EMST-induced division. These are present...