A5076685466- SARS-CoV-2 and COVID-19 Research
- Mast cells and histamine
- COVID-19 Clinical Research Studies
- Asthma and respiratory diseases
- Immune Response and Inflammation
- Influenza Virus Research Studies
- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Immunotherapy and Immune Responses
- Food Allergy and Anaphylaxis Research
- Platelet Disorders and Treatments
- Urticaria and Related Conditions
- Eosinophilic Esophagitis
- Immunodeficiency and Autoimmune Disorders
- Long-Term Effects of COVID-19
- Respiratory viral infections research
- Monoclonal and Polyclonal Antibodies Research
- RNA modifications and cancer
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Click Chemistry and Applications
- Cancer-related gene regulation
- Blood disorders and treatments
- COVID-19 epidemiological studies
Oklahoma Medical Research Foundation
2016-2025
IrsiCaixa
2020-2024
Universitat de Barcelona
2011-2021
Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol
2020-2021
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2011-2020
Abstract Dendritic cells (DCs) initiate immune responses in barrier tissues including lung and skin. Conventional DC (cDC) subsets, CD11b− (cDC1s) or CD11b+ (cDC2s), arise via distinct networks of transcription factors involving IFN regulatory factor 4 (IRF4) IRF8, are specialized for unique functional responses. Using mice which a conditional Irf4 Irf8 allele is deleted CD11c+ cells, we determined whether IRF4 IRF8 deficiency beginning cDC precursors (pre-cDCs) changed the homeostasis...
Humans show significant sex differences in the incidence and severity of respiratory diseases, including asthma virus infection. Sex hormones contribute to female bias type 2 inflammation associated with consistent recent reports that lungs harbor greater numbers GATA-3-dependent group innate lymphoid cells (ILC2s). In this study, we determined whether hormone levels govern numbers, phenotype, function ILC2s murine lung bone marrow (BM). Our data mice significantly ILC2 homeostasis, part due...
We analyzed humoral and cellular immune responses induced by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) messenger RNA (mRNA) vaccines in people with human immunodeficiency virus (HIV; PWH) who had CD4+ T-cell counts <200/µL (HIV<200 group).
Biological sex differences in morbidity upon influenza A virus (IAV) infection are linked to stronger IFN-centered immune responses females, yet the regulatory role of hormone receptors cell subsets is incompletely understood. Lung-resident group 2 innate lymphoid cells (ILC2s) express notably high levels androgen (AR). In IAV infection, ILC2s produce type cytokines and facilitate tissue repair, but they also may be functionally suppressed by 1 cytokines. Here we report magnitude lung ILC2...
Background Mast cells play a critical role in allergic and inflammatory diseases, including exercise-induced bronchoconstriction (EIB) asthma. The mechanism underlying EIB is probably related to increased airway fluid osmolarity that activates mast the release mediators. These mediators then act on bronchial smooth muscle cause bronchoconstriction. In parallel, protective substances such as prostaglandin E2 (PGE2) are also released could explain refractory period observed patients with EIB....
Mass vaccination campaigns reduced COVID-19 incidence and severity. Here, we evaluated the immune responses developed in SARS-CoV-2-uninfected patients with predominantly antibody-deficiencies (PAD) after three mRNA-1273 vaccine doses. PAD were classified based on their immunodeficiency: unclassified primary antibody-deficiency (unPAD, n = 9), common variable immunodeficiency (CVID, 12), combined (CID, 1), thymoma (TID, 1). unPAD healthy controls (HCs, 10) similar vaccine-induced humoral two...
Abstract CD84 is a self-binding receptor from the CD150 (or signaling lymphocyte activation molecule [SLAM]) family that broadly expressed in hematopoietic cells. It has been described adaptors SLAM-associated protein (SAP) and EWS-FLI1–activated transcript 2 (EAT-2) are critical for members’ function. We observed human mast cells express but lack SAP or EAT-2, tyrosine phosphorylated upon FcεRI engagement, release of granule contents reduced when coengaged with LAD2 CD34+-derived In...
Abstract SH3-binding protein 2 (3BP2) is a cytoplasmic adaptor that acts as positive regulator in mast cell FcεRI–dependent signaling. The KIT receptor whose ligand the stem factor necessary for development, proliferation, and survival well optimal IgE-dependent signal. Activating mutations have been associated with several diseases including mastocytosis. In present work, we found 3BP2 silencing impairs signaling pathways, thus affecting phosphoinositide 3-kinase MAPK pathways human cells...
Background: Platelet-activating factor (PAF) is a lipid mediator involved in the pathophysiology of several allergic diseases, for example, amplification mast cell (MC) activation anaphylaxis.Rupatadine an antihistamine with demonstrated anti-PAF effect, although its capacity to inhibit PAF-induced MC degranulation has not been fully evaluated.Objectives: To compare ability rupatadine that desloratadine and levocetirizine confirm dual anti-H1 activity rupatadine.Methods: The human line LAD2...
Abstract Adaptor molecules are essential in organizing signaling and coordinating compartmentalizing their activity. SH3-binding protein 2 (3BP2) is a cytoplasmic adaptor mainly expressed by hematopoietic cells that has been shown to act as positive regulator T, B, NK cell signal transduction. 3BP2 an important of cytotoxic granule release cells. Mast (MCs) similarly degranulate following Ag-dependent aggregation the FcεRI on surface. Activation these induces preformed inflammatory mediators...
The activation and degranulation of mast cells is critical in the pathogenesis allergic inflammation modulation inflammation. Recently, we demonstrated that unconventional long-tailed myosin, MYO1F, localizes with cortical F-actin mediates adhesion migration cells. In this study, show knockdown MYO1F by short hairpin RNA reduces human cell induced both IgE crosslinking stimulation Mas-related G protein-coupled receptor X2 (MRGPRX2), which has been associated pseudoallergic drug reactions,...
Understanding mid-term kinetics of immunity to SARS-CoV-2 is the cornerstone for public health control pandemic and vaccine development. However, current evidence rather based on limited measurements, thus losing sight temporal pattern these changes 1–6 . In this longitudinal analysis, conducted a prospective cohort COVID-19 patients followed up 242 days, we found that individuals with mild or asymptomatic infection experienced an insignificant decay in neutralizing activity persisted six...
Abstract Age is associated with reduced efficacy of vaccines and linked to higher risk severe COVID-19. Here we determined the impact ageing on a SARS-CoV-2 vaccine based stabilised Spike glycoprotein (S-29) that had previously shown high in young animals. Thirteen 18-month-old golden Syrian hamsters (GSH) 22–23-month-old K18-hCAE2 mice were immunised twice S-29 protein AddaVax TM adjuvant. GSH intranasally inoculated either two weeks or four months after booster dose, while all K18-hACE2...
Gastrointestinal stromal tumors (GISTs) represent about 80% of the mesenchymal neoplasms gastrointestinal tract. Most GISTs contain oncogenic KIT (85%) or PDGFRA (5%) receptors. The kinase inhibitor imatinib mesylate is preferential treatment for these tumors; however, development drug resistance has highlighted need novel therapeutic strategies. Recently, we reported that adaptor molecule SH3 Binding Protein 2 (SH3BP2) regulates expression and signaling in human mast cells. Our current...
The use of high-dose intravenous immunoglobulins (IVIGs) as immunomodulators for the treatment COVID-19–affected individuals has shown promising results. IVIG reduced inflammation in these patients, who progressively restored respiratory function. However, little is known about how they may modulate immune responses COVID-19 individuals. Here, we have analyzed levels 41 inflammatory biomarkers plasma samples obtained at day 0 (pretreatment initiation), 3, 7, and 14 from five hospitalized...
Mast cell chemotaxis is essential for recruitment to target tissues, where these cells play an important role in adaptive and innate immunity. Stem factor (SCF) a major chemoattractant mast cells. SCF binds the KIT receptor, thereby triggering tyrosine phosphorylation cytoplasmic domain resulting docking sites SH2 domain-containing molecules, such as Lyn Fyn, subsequent activation of small GTPases Rac that are responsible cytoskeletal reorganization migration. In previous works we have...
Abstract Dendritic cells (DCs) initiate immune responses in barrier tissues including lung and skin. Conventional DC subsets, CD11b−(cDC1s) or CD11b+(cDC2s), arise via distinct networks of transcription factors involving IRF4 IRF8 are specialized for unique functional responses. Using mice which a conditional Irf4 Irf8 allele is deleted CD11c+ cells, we determined if deficiency beginning cDC precursors (pre-cDCs) changed the homeostasis mature DCs pre-DCs lung, dermis spleen....
Abstract Background Influenza is a highly contagious, acute, febrile respiratory infection caused by negative-sense, single-stranded RNA virus, which belongs in the Orthomyxoviridae family. Cigarette smoke (CS) exposure worsens influenza terms of frequency and severity both human animal models. Methods C57BL/6 mice with or without CS for 6 weeks were inoculated intranasally single, non-lethal dose A virus (IAV) A/Puerto Rico/8/1934 (PR8) strain. At 7 10 days after infection, lung mediastinal...
Safe and effective severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines are crucial to fight against the disease 2019 pandemic. Most based on a mutated version of Spike glycoprotein [K986P/V987P (S-2P)] with improved stability, yield immunogenicity. However, S-2P is still produced at low levels. Here, we describe V987H mutation that increases by two-fold production recombinant exposure receptor binding domain (RBD). S-V987H immunogenicity similar in mice golden Syrian...
Most COVID-19 vaccines are based on the SARS-CoV-2 Spike glycoprotein (S) or their subunits. However, S shows some structural instability that limits its immunogenicity and production, hampering development of recombinant S-based vaccines. The introduction K986P V987P (S-2P) mutations increases production trimer, suggesting these two parameters related. Nevertheless, S-2P still molecular it is produced with low yield. Here we described a novel set identified by modeling located in S2 region...
Abstract Purpose Mass vaccination campaigns have reduced the incidence and severity of COVID-19. However, there is limited information about how patients with predominantly antibody-deficiencies (PAD) respond to COVID-19 vaccination. Here, we evaluated humoral cellular responses developed in SARS-CoV-2-naïve PAD individuals after three mRNA-1273 vaccine doses. Methods Patients healthy controls (HCs) were immunized at week 0 (w0) w4. received an additional dose w24. Blood samples collected...
Abstract Safe and effective severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines have been crucial to fight against the disease 2019 pandemic. Most are based on a mutated version of Spike glycoprotein [K986P/V987P (S-2P)] with improved stability, yield immunogenicity. However, S-2P is still produced at low levels. Here, we described novel V987H mutation that increases by two-fold production recombinant exposure receptor binding domain (RBD). S-V987H immunogenicity was...