A5049551235- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- SARS-CoV-2 and COVID-19 Research
- Mosquito-borne diseases and control
- COVID-19 Clinical Research Studies
- HIV-related health complications and treatments
- Viral Infections and Vectors
- Autophagy in Disease and Therapy
- COVID-19 epidemiological studies
- Immune cells in cancer
- Virology and Viral Diseases
- Long-Term Effects of COVID-19
- SARS-CoV-2 detection and testing
- Histone Deacetylase Inhibitors Research
- Herpesvirus Infections and Treatments
- interferon and immune responses
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
Universidad de La Laguna
2019-2024
IrsiCaixa
2020-2021
Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol
2020-2021
The transactive response DNA-binding protein (TARDBP/TDP-43) is known to stabilize the anti-HIV-1 factor, histone deacetylase 6 (HDAC6). TDP-43 has been reported determine cell permissivity HIV-1 fusion and infection acting on tubulin-deacetylase HDAC6. Here, we studied functional involvement of in late stages viral cycle. overexpression TDP-43, virus-producing cells, stabilized HDAC6 (i.e., mRNA protein) triggered autophagic clearance Pr55Gag Vif proteins. These events inhibited particle...
The transactive response DNA-binding protein (TARDBP/TDP-43) influences the processing of diverse transcripts, including that histone deacetylase 6 (HDAC6). Here, we assessed TDP-43 activity in terms regulating CD4+ T-cell permissivity to HIV-1 infection. We observed overexpression wt-TDP-43 increased both mRNA and levels HDAC6, resulting impaired infection independently viral envelope glycoprotein complex (Env) tropism. Consistently, using an Env-mediated cell-to-cell fusion model,...
Abstract In untreated HIV-1-infected individuals, viremia is positively associated with disease progression. However, some viremic non progressors (VNPs) individuals show paradoxical high CD4 + T cell counts. HIV-1 envelope glycoprotein complex (Env) a major cytopathic determinant in viral replication; therefore, we have deeply characterized Env function this rare clinical phenotype. Full-length clones isolated from Viral Load (VL) > 10,000 copies/mL classified as VNPs (n = 15) or rapid...
HIV Nef is a central auxiliary protein in infection and pathogenesis. Our results indicate that HDAC6 promotes the aggresome/autophagic degradation of viral polyprotein Pr55Gag to inhibit HIV-1 production. counteracts this antiviral activity by inducing its subsequently stabilizing Vif proteins. appears neutralize an acidic/endosomal-lysosomal processing does not need downregulation function, since data obtained with non-associated cell-surface Nef-G2A mutant - cytoplasmic location together...
Zika virus (ZIKV) infection and pathogenesis are linked to the disruption of neurogenesis, congenital syndrome microcephaly by affecting neural progenitor cells. Nonstructural protein 5 (NS5) is largest product encoded ZIKV-RNA important for replication immune evasion. Here, we studied potential effects NS5 on microtubules (MTs) autophagy flux, together with interplay histone deacetylase 6 (HDAC6). Fluorescence microscopy, biochemical cell-fractionation combined use HDAC6 mutants, chemical...
The understanding of HIV-1 pathogenesis and clinical progression is incomplete due to the variable contribution host, immune, viral factors. involvement factors has been investigated in extreme phenotypes from rapid progressors long-term non-progressors (LTNPs). Among proteins, envelope glycoprotein complex (Env) concentrated on many studies for its important role immune response first steps replication. In this study, we analyzed 41 Envs 24 patients with different rates loads (VLs),...
Understanding mid-term kinetics of immunity to SARS-CoV-2 is the cornerstone for public health control pandemic and vaccine development. However, current evidence rather based on limited measurements, thus losing sight temporal pattern these changes 1–6 . In this longitudinal analysis, conducted a prospective cohort COVID-19 patients followed up 242 days, we found that individuals with mild or asymptomatic infection experienced an insignificant decay in neutralizing activity persisted six...
Zika virus (ZIKV) infection and pathogenesis are linked to the disruption of neurogenesis, congenital syndrome microcephaly by affecting neural progenitor cells. Nonstructural protein 5 (NS5) is largest product encoded ZIKV-RNA important for replication immune evasion. Here, we studied potential effects NS5 on microtubules (MTs) autophagy flux, together with interplay histone deacetylase 6 (HDAC6). Fluorescence microscopy, biochemical cell-fractionation combined use HDAC6 mutants, chemical...
Abstract The understanding of HIV-1 pathogenesis and clinical progression is incomplete because the variable contribution host, immune viral factors. involvement factors has been investigated in extreme phenotypes from rapid progressors to long-term non-progressors (LTNPs). Among proteins, envelope glycoprotein complex (Env) concentrated many studies for its important role response first steps replication. In this study, we analyzed 41 Envs 24 patients with different rates loads (VLs),...
Abstract The transactive response DNA-binding protein (TDP-43) is an important regulator of mRNA, being reported to stabilize the anti-HIV factor, histone deacetylase 6 (HDAC6). However, little known about role TDP-43 in HIV infection. In this work, we seek for function on regulating CD4+ T cell permissibility We observed that over-expression wt-TDP-43 cells stabilized HDAC6, increasing mRNA and levels antiviral enzyme. Under experimental condition, HIV-1 infection was impaired,...