A5008362073- Biosensors and Analytical Detection
- SARS-CoV-2 detection and testing
- Advanced biosensing and bioanalysis techniques
- Advanced MEMS and NEMS Technologies
- Microfluidic and Bio-sensing Technologies
- Animal Virus Infections Studies
- Acoustic Wave Resonator Technologies
- Viral Infections and Immunology Research
- Bacteriophages and microbial interactions
- Respiratory viral infections research
- Catalytic Processes in Materials Science
- Telemedicine and Telehealth Implementation
- Iron and Steelmaking Processes
- Catalysts for Methane Reforming
- Innovative Microfluidic and Catalytic Techniques Innovation
- Genetic Mapping and Diversity in Plants and Animals
- Wheat and Barley Genetics and Pathology
- Biomedical and Chemical Research
- Nanopore and Nanochannel Transport Studies
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Outbreaks Research
- Mobile Health and mHealth Applications
- RNA and protein synthesis mechanisms
- Data-Driven Disease Surveillance
- Injection Molding Process and Properties
Stockholm University
2017-2022
Science for Life Laboratory
2017-2022
Technical University of Munich
2021
Carl von Ossietzky Universität Oldenburg
2020
Grain weight, an essential yield component, is under strong genetic control and at the same time markedly influenced by environment. Genetic analysis of thousand grain weight (TGW) genome-wide association study (GWAS) was performed with a panel 358 European winter wheat (Triticum aestivum L.) varieties 14 spring using phenotypic data field tests in eight environments. Wide variations were indicated for TGW BLUEs (best linear unbiased estimations) values ranging from 35.9 to 58.2 g mean value...
Emerging tropical viruses have caused serious outbreaks during the recent years, such as Ebola virus (EBOV) in 2014 and most 2018 to 2019 Congo. Thus, immediate diagnostic attention is demanded at point of care resource-limited settings, because performance operational parameters conventional EBOV testing are limited. Especially, their sensitivity, specificity, coverage other disease make them unsuitable for care. Here, a padlock probe (PLP)-based rolling circle amplification (RCA) method...
Abstract The establishment of a robust detection platform for RNA viruses still remains challenge in molecular diagnostics due to their high mutation rates. Newcastle disease virus (NDV) is one such avian with hypervariable genome and multiple genotypes. Classical approaches like isolation, serology, immunoassays RT-PCR are cumbersome, limited terms specificity sensitivity. Padlock probes (PLPs) known allowing the nucleic acid targets specificity, combination Rolling circle amplification...
Antibody characterization is essential for understanding the immune system and development of diagnostics therapeutics. Current technologies are mainly focusing on detection antigen-specific immunoglobulin G (IgG) using bulk singleplex measurements, which lack information other isotypes specificity individual antibodies. Digital immunoassays based nucleic acid amplification have demonstrated superior performance by allowing single molecules in a multiplex sensitive manner. In this study, we...
Abstract BACKGROUND Influenza remains a constant threat worldwide, and WHO estimates that it affects 5% to 15% of the global population each season, with an associated 3 5 million severe cases up 500000 deaths. To limit morbidity economic burden influenza, improved diagnostic assays are needed. METHODS We developed multiplexed assay for detection subtyping seasonal influenza based on padlock probes rolling circle amplification. The simultaneously targets all 8 genome segments 4 circulating...
Abstract Rolling circle amplification (RCA) is a robust isothermal nucleic acid method producing nanocomposites of DNA and inorganic magnesium pyrophosphate precipitates. Although the conformation structure such impact applications, most studies them have been performed at end‐point after exposure to treatment. Here, we use real‐time optomagnetic measurements hydrodynamic size magnetic nanoparticles (MNPs) study growth RCA products grafted onto MNPs as well effect post‐RCA temperature...
We demonstrate real-time experimental evidence for mass underestimation, to analyse high molecular weight biomolecules while using Quartz Crystal Microbalance (QCM) platform. For this, we present an with assay consisting of aptamer-antibody sandwich model norovirus-like particles (NoVLPs). combine this the proximity ligation generate bulky rolling circle products on a gold spot centralized quartz resonator surface. Real-time monitoring build-up reflects that NoVLPs and amplified...
Abstract Sensors for detecting ultra-low concentrations are limited by the efficient transport of target molecules from large sample volumes to small detection regions. We here report a small-format digital DNA sensor in shape microporous membrane that electrically detects substrates with concentration as low 790 zM. This ultra-high sensitivity follows optimising mass specific receptors on across multiple spatial scales. mm-sized membranes support rapid convection volume zone; µm-sized pores...
Despite the large relevance of bimetallic metal nanoparticles for heterogeneous catalysis, relation between their shape and elemental composition remains elusive. Here, we investigate this relationship by implementing applying global optimization methods enhanced with a novel optimal-exchange algorithm. In particular, determine lowest energy chemical orderings PtAu nanoparticles, revealing that most stable changes from highly symmetric structures pure particles to distorted less shapes...